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2010 | 5 | 5 | 565-568
Tytuł artykułu

The first Bulgarian allergen extract from Ambrosia artemisiifolia L.

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Ambrosia artemisiifolia L. is a leading cause of allergies in many countries. In the last decade it has appeared and spread in Bulgaria as well. The aims of this study was to collect pollen from ragweed plants in Bulgaria, prepare the first Bulgarian ragweed allergen, and characterize it. The new allergen was studied in parallel with two other ragweed pollen extracts from the USA and Russia. The protein profile was studied through isoelectric focusing, and the total allergen activity was studied by a skin prick test and a basophil activation test. The proteins of the studied extracts were concentrated in the pI region 3.5–5.5 with an identical band number and location. The allergens showed similar total allergen activity in vivo:14/81 tested patients (17.3 %) were SPT positive either to a single ragweed extract or to all three. The allergens had identical histogram profiles and caused basophil degranulation above the test cut-off. The analysis of the flow cytometry results by parallel line bioassay shows linear dose-response relation between the extracts. The observed immunological properties of the Bulgarian allergen from Ambrosia artemisiifolia provide a possibility to use this product for a reliable diagnosis and effective specific immunotherapy of ragweed allergy in our country.
Wydawca

Czasopismo
Rocznik
Tom
5
Numer
5
Strony
565-568
Opis fizyczny
Daty
wydano
2010-10-01
online
2010-08-20
Twórcy
  • Department of Immunology and Allergy, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria, labalerg@ncipd.org
  • Department of Immunology and Allergy, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria
  • Department of Immunology and Allergy, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria
  • Department of Immunology and Allergy, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria
  • Department of Immunology and Allergy, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria
  • Department of Immunology and Allergy, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria
Bibliografia
  • [1] Mohapatra. S. et al. Weed allergens; in Lockey RF, Bukantz SC, Bousquet J (ed): Allergens and allergen immunotherapy, ed 3, Marcel Dekker, NY, USA, 2004, pp 207–222
  • [2] Jäger S: Ragweed (Ambrosia) sensitization rates correlate with the amount of the inhaled airborne pollen. A 14-year study in Vienna, Austria. Aerobiologia 2000; 16: 149–153 http://dx.doi.org/10.1023/A:1007603321556[Crossref]
  • [3] Taramarcaz P, Lambelet C, Clot B et al: Ragweed (Ambrosia) progression and its health risks: will Switzerland resist this invasion. Swiss Med Wkly 2005; 135: 538–548 [PubMed]
  • [4] Burbach GJ,. Heinzerling LM, Röhnelt C et al: Ragweed sensitization in Europe - GA2LEN study suggests increasing prevalence. Allergy 2009; 64(4): 664–665 http://dx.doi.org/10.1111/j.1398-9995.2009.01975.x[WoS][Crossref]
  • [5] Yankova R, Baltadjieva D, Peneva R et al: Pollen grains of Ambrosia in the air of Sofia, Bulgaria. Aerobiologia 1996; 12: 273–277 [Crossref]
  • [6] Yankova R, Zlatev V, Baltadjieva D et al: Quantitative dynamics of Ambrosia pollen grains in Bulgaria. Aerobiologia 2000; 16: 299–301 http://dx.doi.org/10.1023/A:1007690116728[Crossref]
  • [7] Dimitrov D, Tzonev R: On the distribution of Ambrosia artemisiifolia L. (Asteraceae) in Bulgaria. Phytologia Balcanica 2002; 8(1): 31–33
  • [8] European Pharmacopoeia. Fifth edition 2005, volume 1, 1/2005:1063; 670
  • [9] European Academy of Allergology and Clinical Immunology: Position paper: Allergen standardization and skin tests. Allergy 1993; 48: 48–82 http://dx.doi.org/10.1111/j.1398-9995.1993.tb04698.x[Crossref]
  • [10] Meinert R, Frisher T, Karamanus W et al: Influence of skin prick test criteria on estimation of prevalence and incidence of allergic sensitization in children. Allergy 1994; 49: 526–532 http://dx.doi.org/10.1111/j.1398-9995.1994.tb01124.x[Crossref]
  • [11] Michova, A, Abugalia M, Ivanova Ts, et al. Comparison of two flow cytometry methods for basophil degranulation in patients sensitized to grass pollen. Allergy 2006; 61: 1078–1083 http://dx.doi.org/10.1111/j.1398-9995.2006.01087.x[Crossref]
  • [12] Slater JE: Standardized allergen extracts in the United States; in Lockey RF, Bukantz SC, Bousquet J (ed): Allergens and allergen immunotherapy, ed 3, Marcel Dekker, NY, USA, 2004, pp 421–432
  • [13] Kshirsagar AM: Bioassay; in: Shen-Chung Chow (ed.): Encyclopedia of biopharmaceutical statistics, ed 2, Marcel Dekker, NY, USA, 2003, pp 80–82
  • [14] Fradkin V, Petrunov B, Lavrenchik E, Konstantinova D: Comparative study of the specific activity of pollen allergens found in different geographical areas. Russian Medicine, 1974, 7: 88–90
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-010-0029-2
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