Protective potential of L. acidophilus in murine giardiasis

• Autorzy: Geeta Shukla , Tarveen Kaur , Rakesh Sehgal , Praveen Rishi , Vijay Prabha
• Języki publikacji: EN

Abstrakty

EN

This study describes the in vivo activity of Lactobacillus acidophilus in Giardia lamblia infected BALB/c mice. Experimentally, it was observed that daily administration of lactobacilli 7 days before or in simultaneous inoculation with Giardia trophozoites efficiently reduced G. lamblia infection in mice. More specifically, excretion of Giardia cysts were reduced significantly in probiotic-treated groups, and resolution of infection was observed by day 21 post-inoculation. It was also observed that the lactobacillus count increased tremendously and continuously in faeces of all probiotic-fed mice, and was significantly higher as compared with that in control mice. Histological analysis of microvilli membrane integrity revealed that probiotic administration also protected mice against parasite-induced mucosal damage, whereas Giardia-infected mice had severe villous atrophy, oedema, vacuolation and ileitis. Immunologically, the anti-Giardia serum IgG level was not stimulated significantly by probiotic treatment administered both prior to and simultaneous with Giardia infection, but remained high after the infection peak. Taken together, the data demonstrates the anti-giardial effect of the probiotic in vivo by modulation of the intestinal epithelial cells, inhibiting the colonization of Giardia trophozoites and thereby reducing the severity of Giardia infection.

Słowa kluczowe

EN

Probiotic; Giardiasis; Lactobacillus acidophilus; Murine model

• Czasopismo: Open Medicine
• Rocznik: 2010
• Tom: 5
• Numer: 4
• Strony: 456-463

Daty

wydano

2010-08-01

online

2010-05-30

Twórcy

autor

Geeta Shukla

• Department of Microbiology, Panjab University, Chandigarh, 160014, India,

autor

Tarveen Kaur

• Department of Microbiology, Panjab University, Chandigarh, 160014, India

autor

Rakesh Sehgal

• Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India

autor

Praveen Rishi

• Department of Microbiology, Panjab University, Chandigarh, 160014, India

autor

Vijay Prabha

• Department of Microbiology, Panjab University, Chandigarh, 160014, India

Bibliografia

• [1] Eckmann L., Mucosal defences against Giardia, Parasite Immunology, 2003, 25, 259–270 http://dx.doi.org/10.1046/j.1365-3024.2003.00634.x[Crossref]
• [2] Upcroft P., Upcroft J. A., Drug targets and mechanisms and of resistance in the anaerobic protozoa, Clinical Microbiol. Rev., 2001,14, 150–164 http://dx.doi.org/10.1128/CMR.14.1.150-164.2001[Crossref]
• [3] Eckmanm L., Gillin F.D., Microbes and microbial toxis: paradigms for microbial-mucosal interactions. Pathophysiological aspects of enteric infections with the lumen dwelling protozoan Giardia lamblia, Am. J. Physio. Gestroenterology, 2001, 280, G1–G6
• [4] Khanna R., Vinayak V.K., Mehta F.S., Kumkum R.K., Nain C.K., Giardia lamblia infection in immunosuppressed animals causes severe alterations to brush border membrane enzymes, Dig. Dis. Sci., 1998,33, 1147–1152 http://dx.doi.org/10.1007/BF01535792[Crossref]
• [5] Walia B.N., Ganguly N.K., Mahajan R.C., Kimar D., Madan I.J., Gambhir S.K., Kanwar S.S., Morbidity in pre school giardia cyst excretors, Trop. Geograp. Med., 1986, 38(4), 367–370
• [6] Gardner T.B., Hill D.R., Treatment of Giardiasis, Clin. Microbiol. Rev., 2001,14: 114–128 http://dx.doi.org/10.1128/CMR.14.1.114-128.2001[Crossref]
• [7] Calzada F., Meckes M., Cedillo-Rivera R., Antiamoebic and antigiardial activity of plant flavonoids, Planta. Med., 1999, 65, 78–80 http://dx.doi.org/10.1055/s-2006-960445[Crossref]
• [8] Aggarwal A.K., Singh M., Gupta N., Management of giardiasis by an immuno-modulatory herbal drug Pippali rasayana, J. Ethropharmacol., 1994, 44,143–146 http://dx.doi.org/10.1016/0378-8741(94)01181-8[Crossref]
• [9] Kaneda Y., Torii M., Tanaka T., Aikawa M., In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis, Ann. Trop. Med. Parasitol., 1991, 85, 417–425
• [10] Oyetayo V.O., Adetuyi F.C., Akinyosoye F.A., Safety and protective effect of Lactobacillus acidophilus and Lactobacillus casei used as probiotic agent in vivo, Afr. J. Biotechnol. 2003, 2 (11), 448–452
• [11] Aattouri N., Bouras M., Tome D., Marcos A., Lemonnier D., Oral ingestion of lactic acid bacteria by rats increases lymphocyte proliferation and interferon - production, British. J., 2001, 87:367–373
• [12] Koop-Hoolihan L., Prophylactic and therapeutic uses of probiotics: A review, J. Am. Dietetic. Assoc., 2001, File: //G:/ Intern 1
• [13] Diamond L.S., Technique of axenic culture of E. histolytica schaudinn, 1903 and E. histolytica like amoebae, J. Parasitol., 1963, 64, 1047–1056
• [14] Benyacoub J., Perez P.F., Rochat F., Saudan K.Y., Reuteler G., Antille N., Humen M., De Antoni G.L., Cavadini C., Blum S., Schiffrin E.J., Enterococcus faecium SF68 enhances the immune response to Giardia intestinalis in mice, J. of Nutrition, 2005, 135, 1171–1176
• [15] Tiwari R.P., Hoondal G.S., Tewari R., Laboratory Techniques in Microbiology and Biotechnology, 2001, Abhishek Publication
• [16] Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., Protein measurement with folin’s phenol reagent, J. Bio. Chem. 1951, 193, 265–275
• [17] Hudson L., Hay F.C., The lymphocyte: its role and function, Practical Immunology, 1980, Third edition, Blackwell Scientific Publication, Oxford, London
• [18] Oyetayo V.O., Oyetayo F.L., Potential of probiotics as biotherapeutic agents targeting the innate immune system, Afr J Biotechnol 2005, 4 (2), 123–127
• [19] Singer S.M., Nash T.E., T-cell dependent control of acute Giardia lamblia infections in mice, Infect. Immunity, 2000, 68, 170–175 http://dx.doi.org/10.1128/IAI.68.1.170-175.2000[Crossref]
• [20] Humen M.A.L., De Antoni J., Benyacoud M.B., Costas M.I., Cardozo L., Kozubsky K., Saudan A., Doenzli-Bruand S., Blum E.J., Schiffrin P.F., Perez Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo, Infect. Immun., 2005, 73, 1265–1269 http://dx.doi.org/10.1128/IAI.73.2.1265-1269.2005[Crossref]
• [21] Perdigon G., Vintini E., Alvarez S., Medina M., Medici M., Study of the possible mechanisms involved in the mucosal immune system activation by lactic acid bacteria, J. Dairy Sci., 1999, 82, 1108–1114 http://dx.doi.org/10.3168/jds.S0022-0302(99)75333-6[Crossref]
• [22] Salminen S., Bouley C. Boutron-Ruault M.C., Functional food science and gastrointestinalphysiology and function, Br. J. Nutr., 1998, (suppl) s, 147–71
• [23] Isolauri E., Kalia M., Arvola T., Diet during rotavirus enteritis affects jejunal permeability to macromolecules in suckling rats, Pediatr. Res., 1993, 33, 548–53 http://dx.doi.org/10.1203/00006450-199306000-00002[Crossref]
• [24] Farthing M.J.G., Diarrhoeal disease: current concepts and future challenges (pathogenesis of giardiasis), Trans. R. Soc. Trop. Med. Hyg., 1993, 87, S17–S21 http://dx.doi.org/10.1016/0035-9203(93)90531-T[Crossref]

Identyfikatory

DOI

10.2478/s11536-009-0139-x