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2009 | 4 | 1 | 59-64
Tytuł artykułu

Midnight salivary cortisol, measured by highly sensitive electrochemiluminescence immunoassay, for the diagnosis of Cushing’s syndrome

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the present study is to evaluate the measurement of midnight salivary cortisol as a method of screening for Cushing’s syndrome (CS). Here we tested the performance of a highly sensitive electrochemiluminescence immunoassay (ECLIA) for midnight salivary cortisol measurement in an extensive clinical study (n=104). Three groups were investigated: 30 patients with CS, 34 with obesity and 40 healthy normal weight controls. All of them collected saliva samples at 24:00 h and urine samples over the same day (24 hour period). An electrochemiluminescence immunoassay was used to measure salivary cortisol. Mean midnight salivary cortisol in healthy volunteers, obese patients and patients with CS was 8.33 ± 3.62, 8.13 ± 4.47 and 33.11 ± 21.68 nmol/l, respectively. No significant difference was found between midnight salivary cortisol in healthy and obese subjects (P>0.05). In contrast, salivary cortisol at midnight was significantly higher in patients with CS (P<0.001) as compared to both other groups. The cut-off point of 14.2 nmol/l yielded a sensitivity of 93.3% and a specificity of 94.2% (AUCROC=0.984 ± 0.01(0.965-1.000). A strong positive correlation between midnight salivary cortisol and urinary free cortisol has been found in the CS group (r=0.686, P<0.0001). Our results demonstrate that measurement of midnight salivary cortisol could be successfully used as a first-line screening method for CS. Our data approve ECLIA as a simple, reliable and timesaving method for the assessment of salivary cortisol. Automated measurement of midnight salivary cortisol by ECLIA would facilitate the routine practice in the screening for CS.
Wydawca

Czasopismo
Rocznik
Tom
4
Numer
1
Strony
59-64
Opis fizyczny
Daty
wydano
2009-03-01
online
2009-02-11
Twórcy
autor
  • Clinical Centre of Endocrinology and Gerontology, Medical University, 1303, Sofia, Bulgaria
  • Clinical Centre of Endocrinology and Gerontology, Medical University, 1303, Sofia, Bulgaria
Bibliografia
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  • [2] Raff H., Raff J.L., Findling J.W., Late-night salivary cortisol as a screening test for Cushing’s syndrome, J. Clin. Endocrinol. Metab., 1998, 83, 2681–86 http://dx.doi.org/10.1210/jc.83.8.2681[Crossref]
  • [3] Castro M., Elias P.C., Quidute A.R., Halah F.P., Moreira A.C., Out-patient screening for Cushing’s syndrome: the sensitivity of combination of circadian rhythm and overnight dexamethasone suppression salivary cortisol tests, J. Clin. Endocrinol. Metab., 1999, 84, 878–82 http://dx.doi.org/10.1210/jc.84.3.878[Crossref]
  • [4] Papanicolaou D.A., Mullen N., Kyrou I., Nieman L.K., Nighttime salivary cortisol: A useful test for the diagnosis of Cushing’s syndrome, J. Clin. Endocrinol. Metab., 2002, 87, 4515–21 http://dx.doi.org/10.1210/jc.2002-020534[Crossref]
  • [5] Putignano P., Toja P., Dubini A., Giraldi F.P., Corsello S.M., Cavagnini F., Midnight salivary cortisol versus urinary free and midnight serum cortisol as screening tests for Cushing’s syndrome, J. Clin. Endocrinol. Metab., 2003, 88, 4153–57 http://dx.doi.org/10.1210/jc.2003-030312[Crossref]
  • [6] Yaneva M., Mosnier-Pudar H., Dugue M.A., Grabar S., Fulla Y., Bertagna X., Midnight salivary cortisol for the initial diagnosis of Cushing’s syndrome of various causes, J. Clin. Endocrinol. Metab., 2004, 89, 3345–51 http://dx.doi.org/10.1210/jc.2003-031790[Crossref]
  • [7] Gafni R.I., Papanicolau D.A., Nieman L.K., Night time salivary cortisol measurement as a simple, noninvasive, outpatient screening test for Cushing’s syndrome in children and adolescents, J. Pediatr., 2000, 137, 30–35 http://dx.doi.org/10.1067/mpd.2000.106226[Crossref]
  • [8] MartinelliJr C.E., Sader S.L., Oliveira E.B., Daneluzzi J.C., Moreira A.C., Salivary cortisol for screening of Cushing’s syndrome in children, Clin. Endocrinol. (Oxf), 1999, 51, 67–71 http://dx.doi.org/10.1046/j.1365-2265.1999.00749.x[Crossref]
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  • [12] Pirich K, Vierhapper H., 24-hour serum concentration profile of cortisol in patients with Cushing’s disease, Exp. Clin. Endocrinol., 1998, 92, 275–9 http://dx.doi.org/10.1055/s-0029-1210815[Crossref]
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  • [15] Vining R.F., McGinley R.A., Maksvytis J.J., Ho K.Y., Salivary cortisol - a better measure of adrenal cortical function than serum cortisol, Ann. Clin. Biochem., 1983, 20, 329–335 [PubMed][Crossref]
  • [16] Vining R.F., McGinley R.A., The measurement of hormones in saliva: possibilities and pitfalls, J. Steroid. Biochem., 1987, 27, 81–94 http://dx.doi.org/10.1016/0022-4731(87)90297-4[Crossref]
  • [17] Vining R.F., McGinley R.A., Transport of steroids from blood to saliva. In Immunoassays of steroids in saliva, pp. 56–63. Eds Read GF, Riad-Fahmy D, Walker RF & Griffiths K. Cardiff: Alpha Omega Publishing Ltd, 1984
  • [18] Chen Y.M., Cintron N.M., Whitson P.A., Long-term storage of salivary cortisol samples at room temperature, Clin. Chem. 1992, 38, 304–305 [PubMed]
  • [19] Mosnier-Pudar H., Thomopoulos P., Bertagna X., Fournier C., Guiban D., Luton J,P., Long-distance and long-term follow-up of a patient with intermittent Cushing’s disease by salivary cortisol measurements, Eur. J. Clin. Endocrinol., 1995, 133, 313–316 http://dx.doi.org/10.1530/eje.0.1330313[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-009-0004-y
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