PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
Czasopismo
2009 | 4 | 2 | 245-252
Tytuł artykułu

The production of reactive oxygen species in peripheral blood neutrophils is modulated by airway mucous

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Neutrophils are a major source of reactive oxygen species (ROS). The role of airway mucous on ROS production is unknown. The aim of our study was to investigate the direct influence of bronchoalveolar lavage fluid (BALF) and induced sputum (IS) alone or in combination with chemical/biological stimulus on ROS production in peripheral blood neutrophils during chronic obstructive pulmonary disease (COPD). Neutrophils were isolated from peripheral blood of 47 patients with moderate COPD and 14 healthy individuals (HI). BALF/RPMI (1:1) or IS/RPMI (1:1) from COPD patients were used to stimulate neutrophils alone or in combination with phorbolmyristate- acetate (PMA) (0.1–30 nM) or Staphylococcus aureus bacteria (0.7–500 bact/neutrophil). Relative generation of ROS was measured flow cytometrically. BALF/RPMI and in combination with relatively low PMA or all bacteria concentrations stimulated ROS; while, combination with relatively high PMA concentrations suppressed ROS in of COPD patients and HI. IS/RPMI and its combination with PMA inhibited ROS generation in both groups; whereas, IS stimulated or had a tendency to stimulate ROS production with relatively high bacteria concentrations. In conclusion, BALF and IS directly or in combination with chemical/biological factors modulated ROS production. This effect was stronger in neutrophils from COPD patients and depended on chemical/biological stimulus intensity.
Słowa kluczowe
EN
BALF   COPD   IS   Neutrophils   ROS  
Wydawca
Czasopismo
Rocznik
Tom
4
Numer
2
Strony
245-252
Opis fizyczny
Daty
wydano
2009-06-01
online
2009-03-27
Twórcy
  • Laboratory of Pulmonology, Institute for Biomedical Research, Kaunas University of Medicine, LT-50009, Kaunas, Lithuania , agne.babusyte@gmail.com
  • Institute of Immunology, Universtiy of Veterinary Medicine, D-30173, Hannover, Germany
  • Department of Pulmonology and Immunology, Kaunas Medical University Hospital, LT-50009, Kaunas, Lithuania
Bibliografia
  • [1] Global Initiative for Chronic Obstructive Lung Disease. Global strategy for the diagnosis, management and prevention of chronic obstructive pulmonary disease, NHLBI/WHO Workshop Report., 2007, http://www.goldcopd.com
  • [2] Drost E.M., Skwarski K.M., Sauleda J., Soler N., Roca J., Agusti A., et al., Oxidative stress and airway inflammation in severe exacerbations of COPD, Thorax., 2005, 60, 293–300 http://dx.doi.org/10.1136/thx.2004.027946[Crossref]
  • [3] MacNee W., Airway infection does not accelerate decline in lung function in chronic obstructive pulmonary disease, Am. J. Respir. Crit. Care. Med., 2001, 164, 1758–1760
  • [4] Kulkarni N., Cooke M.S., Grigg J., Neutrophils in induced sputum from healthy children: Role of IL-8 and oxidative stress, Repir. Med., 2007, 101, 2108–2112 http://dx.doi.org/10.1016/j.rmed.2007.05.018[Crossref]
  • [5] Noguera A., Batle S., Miralles C., Iglesias J., Busquets X., MacNee W., et al., Enhanced neutrophil response in chronic obstructive pulmonary disease, Thorax, 2001, 56, 432–437 http://dx.doi.org/10.1136/thorax.56.6.432[Crossref]
  • [6] Rahman I., Oxidative stress and gene transcription in asthma and chronic obstructive pulmonary disease: antioxidant therapeutic targets, Curr. Drug. Targets. Inflamm. Allergy., 2002, 1, 291–315 http://dx.doi.org/10.2174/1568010023344607[Crossref]
  • [7] Repine J.E., Bast A., Lankhorst I., Oxidative stress in chronic obstructive pulmonary disease, Oxidative Stress Study Group, Am. J. Respir. Crit. Care. Med., 1997, 156, 341–357
  • [8] Babusyte A., Stravinskaite K., Jeroch J., Lotvall J., Sakalauskas R., Sitkauskiene B., Patterns of airway inflammation and MMP-12 expression in smokers and ex-smokers with COPD, Respir Res., 2007, 8, 81 http://dx.doi.org/10.1186/1465-9921-8-81[WoS][Crossref]
  • [9] Bowler R.P., Barnes P.J., Crapo J.D., The role of oxidative stress in chronic obstructive pulmonary disease, COPD., 2004, 1, 255–277 http://dx.doi.org/10.1081/COPD-200027031[Crossref]
  • [10] Keatings V.M., Collins P.D., Scott D.M., Barnes P.J., Differences in interleukin-8 and tumour necrosis factor-alpha in induced sputum from patients with chronic obstructive pulmonary disease or asthma, Am. J. Respir. Crit. Care. Med., 1996, 153, 530–534
  • [11] Standards for the diagnosis and treatment of patients with COPD, ATS/ERS Guidelines., 2004, http://www.ersnet.org
  • [12] Standardised methodology of sputum induction and processing, ATS/ERS Guidelines., 2002, http://www.ersnet.org
  • [13] Kaplan L.A., Pesce A.J., Clinical Chemistry. Theory, analysis and correlation, 3rd Edition., 1996
  • [14] Trotter J., WinMDI 2.8 software package, 2000, http://www.facs.scripps.edu/software.html.
  • [15] Tkacova R., Kluchova Z., Joppa P., Petrasova D., Molcanyiova A., Systemic inflammation and systemic oxidative stress in patients with acute exacerbations of COPD, Respir. Med., 2007, 101, 1670–1676 http://dx.doi.org/10.1016/j.rmed.2007.03.005[Crossref][WoS]
  • [16] Yoshikawa T., Dent G., Ward J., Angco G., Nong G., Nomura N., et al., Impaired neutrophil chemotaxis in chronic obstructive pulmonary disease, Am. J. Respir. Crit. Care. Med., 2007, 175, 473–479 http://dx.doi.org/10.1164/rccm.200507-1152OC[Crossref]
  • [17] Fialkow L., Wang Y., Downey G.P., Reactive oxygen and nitrogen species as signaling molecules regulating neutrophil function, Free. Radic. Biol. Med., 2007, 42, 153–164 http://dx.doi.org/10.1016/j.freeradbiomed.2006.09.030[WoS][Crossref]
  • [18] Koay M.A., Christman J.W., Segal B.H., Venkatakrishnan A., Blackwell T.R., Holland S.M., et al., Impaired pulmonary NF-κB activation in response to lipopolysaccharide in NADPH oxidase-deficient mice, Infect Immun., 2001, 69, 5991–5996 http://dx.doi.org/10.1128/IAI.69.10.5991-5996.2001[Crossref]
  • [19] Yang S., Panoskaltsis-Mortari A., Shukla M., Blazar B.R., Haddad I.Y., Exuberant inflammation in nicotinamide adenine dinucleotide phosphateoxidase- deficient mice after allogeneic marrow transplantation, J. Immunol., 2002, 168, 5840–5847
  • [20] Babior B.M., Lambeth J.D., Nauseef W., The neutrophil NADPH oxidase, Arch. Biochem. Biophys., 2002, 397, 342–344 http://dx.doi.org/10.1006/abbi.2001.2642[Crossref]
  • [21] Cianchetti S., Bacci E., Ruocco L., Bartoli M.L., Ricci M., Pavia T., Dente F.L, Franco A., Vagaggini B., Paggiaro P.L., Granulocyte markers in hypertonic and isotonic saline-induced sputum of asthmatic subjects, Eur. Respir. J., 2004, 24, 1018–1024 http://dx.doi.org/10.1183/09031936.04.00139503[Crossref]
  • [22] Rizoli S.B., Rotstein O.D., Parodo J., Phillips M.J., Kapus A., Hypertonic inhibition of exocytosis in neutrophils: central role for osmotic actin skeleton remodeling., Am. J. Physiol. Cell. Physiol., 2000, 279, C619–C633
  • [23] Junger W.G., Hoyt D.B., Davis R.E., Herdon-Remelius C., Namiki S., Junger H., Loomis W., Altman A., Hypertonicity regulates the function of human neutrophils by modulating chemoattractant receptor signaling and activating mitogen-activated protein kinase p38, J. Clin. Invest., 1998, 109, 2768–2779 http://dx.doi.org/10.1172/JCI1354[Crossref]
  • [24] Rahman I., Oxidative stress, transcription factors and chromatin remodelling in lung inflammation, Biochem. Pharmacol., 2002, 64, 935–942 http://dx.doi.org/10.1016/S0006-2952(02)01153-X[Crossref]
  • [25] MacNee W., Oxidative stress and lung inflammation in airways disease, Eur. J. Pharmacol., 2001, 429, 195–207 http://dx.doi.org/10.1016/S0014-2999(01)01320-6[Crossref]
  • [26] Quint J.K., Wedzicha J.A., The neutrophil in chronic obstructive pulmonary disease, J. Allergy. Clin. Immun., 2007, 119, 1065–1071 http://dx.doi.org/10.1016/j.jaci.2006.12.640[Crossref]
  • [27] O’Donnell R., Breen D., Wilson S., Djukanovic R., Inflammatory cells in the airways in COPD, Thorax, 2006, 61, 448–454 http://dx.doi.org/10.1136/thx.2004.024463[Crossref]
  • [28] Moodley Y.P., Krishnan V., Lalloo U.G., Neutrophils in induced sputum rise from central airways, Eur. Respir. J., 2000, 15, 36–40 http://dx.doi.org/10.1183/09031936.00.15103600[Crossref]
  • [29] Gaudry M., Caon A.C., Gilbert C., Lille S., Naccache P.H., Evidence for the involvement of tyrosine kinases in the locomotory responses of human neutrophils, J. Leukocyte. Biol., 1992, 51, 103–108
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-008-0091-1
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.