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2008 | 3 | 4 | 470-474
Tytuł artykułu

The comparison of cultures, widal agglutination test and polymerase chain reaction as a diagnostic tool in typhoid fever

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Typhoid fever caused by Salmonella typhi, paratyphi A and B, is an important cause of morbidity and mortality in many developing countries. A rapid and sensitive method for the detection of S. typhi is essential for early diagnosis of typhoid fever and effective therapy. In this study 45 febrile patients who were suspected to have enteric fever were enrolled, and the results of blood cultures, widal agglutination tests and Polymerase Chain Reaction in these cases were evaluated. Group I consisted of 11 patients with diseases other than salmonella infections, group II represented 6 patients with positive cultures, and group III represented 28 patients with negative blood cultures negative but who were clinically suspected cases that had a medical history of using variable antimicrobial agents. Two positive PCR results were present; one of them was in culture positive group (16,6%) and the other was in culture negative group (3,5%). In our study widal agglutination tests and cultures were found not to be helpful in differential dignosis. Although PCR based detection of S. typhi is reported to be a sensitive and specific test for the diagnosis of enteric fever, in our study the benefit of this method in the diagnosis of especially patients who were treated with antimicrobial therapy was not clearly determined. Other methods to increase sensitiviy and specificity to levels such as those of real time PCR should be developed and large-scaled studies should be done in endemic and non-epidemic regions.
Wydawca
Czasopismo
Rocznik
Tom
3
Numer
4
Strony
470-474
Opis fizyczny
Daty
wydano
2008-12-01
online
2008-10-22
Twórcy
autor
  • Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Hacettepe University, Turkey, Ankara
autor
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
autor
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
autor
  • Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Hacettepe University, Turkey, Ankara
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Hacettepe University, Turkey, Ankara
Bibliografia
  • [1] Crump JA, Luby SP, Mintz ED. The global burden of typhoid fever. Bull World Health Organ 2004; 82: 346–53.
  • [2] Gasem MH,. Dolmans WM, Isbandrio BB et al. Culture of Salmonella typhi and Salmonella paratyphi from blood and bone marrow in suspected typhoid fever. Trop Geogr Med 1995; 47: 164–67.
  • [3] Wain J, Diep TS, Ho VA, et al. Quantitation of bacteria in blood of typhoid fever patients and relationship between counts and clinical features, transmissibility, and antibiotic resistance. J Clin Microbiol 1998; 36: 1683–87.
  • [4] Kumar A., Arora V., Bashampoo A et al. Detection of Salmonella typhi by polymerase chain reaction: Implications in diagnosis of typhoid fever. Infection, Genetics and Evolution 2 (2002) 107–110. http://dx.doi.org/10.1016/S1567-1348(02)00090-4[Crossref]
  • [5] Olopoenia LA, King AL. Widal agglutination test - 100 years later: still plagued by controversy. Postgrad Med J 2000;76:80–84. http://dx.doi.org/10.1136/pmj.76.892.80[Crossref]
  • [6] Tupasi TE, Lucas-Aquino R, Mendoza MT et al. Clinical Application of the Widal Test. Phil J Microbiol Infect Dis 1991; 20(1):23–26.
  • [7] World Health Organization. Background document: The diagnosis, treatment and prevention of typhoid fever. WHO/V & B / 2007; 03: 1–38.
  • [8] Gilman, R.H., Terminel, M., Levine, M.M. et al. Relative efficacy of blood, urine, rectal swab, bonemarrow, and rose-spot cultures for recovery of Salmonella typhi in typhoid fever. Lancet 1975; 1 1211–1213. http://dx.doi.org/10.1016/S0140-6736(75)92194-7[Crossref]
  • [9] Hoffman, S.L., Punjabi, N.H., Rockhill, R.C. et al. Duodenal string capsule culture compared with bone-marrow, blood and rectal cultures for diagnosis typhoid and a paratyhphoid fever. J. Infect. Dis. 1984:149: 157–161.
  • [10] Cocolin L., Manzano M., Astori G et al. A highly sensitive and fast nonradioactive method for the detection of polymerase chain reaction products from Salmonella serovars, such as Salmonella Typhi, in blood specimens. FEMS Immunol and MedicMicrobiol 1998; 22: 233–239. http://dx.doi.org/10.1111/j.1574-695X.1998.tb01211.x[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-008-0052-8
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