Comparison of octenidine dihydrochloride (Octenisept®), polihexanide (Prontosan®) and povidon iodine (Betadine®) for topical antibacterial effects in Pseudomonas aeruginosa-contaminated, full-skin thickness burn wounds in rats

• Autorzy: Fatih Uygur , Mustafa Özyurt , Rahmi Evinç , Tugrul Hosbul , Bahattin Çeliköz , Tuncer Haznedaroglu
• Języki publikacji: EN

Abstrakty

EN

Pseudomonas aeruginosa is one of the most frequently isolated organisms from infected burn wounds and a significant cause of nosocomial infection and septic mortality among burn patients. In this animal study, three antiseptic agents which were Octenidine dihydrochloride (Octenisept®, Schülke & Mayr, Norderstedt, Germany), polyhexanide (Prontosan®, B. Braun, Melsungen AG, Germany) and povidon iodine (Betadine, Purdue Pharma L.P, Stamford, USA) were compared to assess the antiseptic effect of their applications on experimental burn wounds in in rats contaiminated with P. aeruginosa. All treatment modalities were effective against P. aeruginosa because there were significant differences between treatment groups and control groups. The mean eschar concentrations were not different between polyhexanide and povidon iodine groups, but there were significant differences between the octenidine dihydrochloride group and the other treatment groups, indicating that the Octenidine dihydrochloride significantly eliminated P. aeruginosa more effectively in the tissues compared to the to other agents. All treatment modalities were sufficient to prevent the P. aeruginosa invasion into the muscle and to cause systemic infection. In conclusion, Octenidine dihydrochloride is the most effective antiseptic agent in the treatment of the P. aeruginosa-contaminated burn wounds; Octenidine dihydrochloride can be considered as a treatment choice because of its peculiar ability of limit the frequency of replacing wound dressings.

Słowa kluczowe

EN

Pseudomonas aeruginosa; Burn wounds; Antiseptic

• Czasopismo: Open Medicine
• Rocznik: 2008
• Tom: 3
• Numer: 4
• Strony: 417-421

Daty

wydano

2008-12-01

online

2008-10-22

Twórcy

autor

Fatih Uygur

• Department of Plastic and Reconstructive Surgery and Burn Unit, Gülhane Military Medical Academy and Medical Faculty, Haydarpaşa Training Hospital, 34668, Istanbul, Turkey,

autor

Mustafa Özyurt

• Department of Microbiology and Clinical Microbiology, Gülhane Military Medical Academy and Medical Faculty, Haydarpaşa Training Hospital, 34668, Istanbul, Turkey

autor

Rahmi Evinç

• Department of Plastic and Reconstructive Surgery and Burn Unit, Gülhane Military Medical Academy and Medical Faculty, Haydarpaşa Training Hospital, 34668, Istanbul, Turkey

autor

Tugrul Hosbul

• Department of Microbiology and Clinical Microbiology, Gülhane Military Medical Academy and Medical Faculty, Haydarpaşa Training Hospital, 34668, Istanbul, Turkey

autor

Bahattin Çeliköz

• Department of Plastic and Reconstructive Surgery and Burn Unit, Gülhane Military Medical Academy and Medical Faculty, Haydarpaşa Training Hospital, 34668, Istanbul, Turkey

autor

Tuncer Haznedaroglu

• Department of Microbiology and Clinical Microbiology, Gülhane Military Medical Academy and Medical Faculty, Haydarpaşa Training Hospital, 34668, Istanbul, Turkey

Bibliografia

• [1] Pruitt, B. A., Jr., Infection and the burn patient, Br. J. Surg., 1990, 77, 1081–1082 http://dx.doi.org/10.1002/bjs.1800771002[Crossref]
• [2] Pruitt, B. A., Jr., The diagnosis and treatment of infection in the burn patient, Burns Incl. Therm. Inj. 1984, 11, 79–91 [PubMed]
• [3] Greefield E., McManus AT., Infection complication, prevention and strategies for their control, Nurs. Clin. North. Am., 1997, 32, 297–309
• [4] McManus A., Twenty-five year review of Pseudomonas aeruginosa bacteremia in a burn centre, Eur. J. Clin. Microbiol., 1985, 4, 219–223 http://dx.doi.org/10.1007/BF02013601[Crossref]
• [5] Botzenhart K., Doring G., Ecology and epidemiology of Pseudomonas aeruginosa. In., M. Campo, editor. Pseudomonas aeruginosa as an Opportunistic Pathogen., New York, Plenum Press, 1993, 1–18
• [6] Revathi G., Puri J., Jain B., Bacteriology of burns., Burns., 1998, 24, 347–349 http://dx.doi.org/10.1016/S0305-4179(98)00009-6[Crossref]
• [7] Tredget E., Shankowsky H., Rennie R., Burrell R., Logsetty S., Pseudomonas infections in the thermally injured patient, Burns, 2004, 30, 3–26 http://dx.doi.org/10.1016/j.burns.2003.08.007[Crossref]
• [8] Walker HL., Mason AD., A standard animal burn, J. Trauma, 1968, 8, 1049–51 [Crossref]
• [9] Herndon DN., editor.Herndon’s total burn care. 2nd ed. London, W.B. Saunders Co., 2001, p. 98–169
• [10] Houari A., Di Martino P., Effect of chlorhexidine and benzalkonium chloride on bacterial biofilm formation, Lett Appl Microbiol., 2007 Dec, 45(6), 652–6. Epub 2007 Oct 17 http://dx.doi.org/10.1111/j.1472-765X.2007.02249.x[Crossref]
• [11] DeQueiroz GA., Day DF., Antimicrobial activity and effectiveness of a combination of sodium hypochlorite and hydrogen peroxide in killing and removing Pseudomonas aeruginosa biofilms from surfaces, J. Appl Microbiol., 2007 Oct, 103(4), 794–802 [WoS][Crossref]
• [12] Sreenivasan PK., Chorny RC., The effects of disinfectant foam on microbial biofilms, Biofouling. 2005, 21(2), 141–149 http://dx.doi.org/10.1080/08927010500155694[Crossref]
• [13] Ulkür E., Oncül O., Karagöz H., Celiköz B., Cavu§lu S., Comparison of silver-coated dressing (Acticoat), chlorhexidine acetate 0.5% (Bactigrass), and silver sulfadiazine 1% (Silverdin) for topical antibacterial effect in Pseudomonas aeruginosa-contaminated, full-skin thickness burn wounds in rats, J. Burn Care Rehabil., 2005, 26(5), 430–433 http://dx.doi.org/10.1097/01.bcr.0000176879.27535.09
• [14] Kramer A. et al., Comparison of the toxicity of Lavasept and selected antiseptic agents, Hyg. Med., 1993, 18, 9–16
• [15] Harke HP., Octenidine dihydrochloride, properties of a new antimicrobial active agent, Zbl. Hyg., 1989, 188, 188–93 [PubMed]
• [16] Sedlock DM., Bailey DM., Microbicidal activity of octenidine hydrochloride, a new alkanediylbis[pyridine] germicidal agent, Antimicrob. Agents. Chemother., 1985, 28, 786–90 [PubMed]
• [17] Willenegger H., Local antiseptics in surgery - rebirth and advances, Unfallchirurgie., 1994, 20, 94–110 http://dx.doi.org/10.1007/BF02588150[Crossref]
• [18] Ikeda T., Tazuke S., Watanabe M., Interaction of biologically active molecules with phospholipid membranes, I. Fluorescence depolarization studies on the effect of polymeric biocide bearing biguanide groups in the main chain, Biochim. Biophys. Acta., 1983, 735, 380–6 http://dx.doi.org/10.1016/0005-2736(83)90152-9[Crossref]
• [19] Broxton P., Woodcock PM., Heatley F., Gilbert P., Interaction of some polyhexamethylene biguanides and membrane phospholipids in Escherichia coli., J. Appl. Bacteriol., 1984, 57, 115–24 [Crossref]
• [20] Rietkötter J., Körber A., Grabbe S., Dissemond J., Eradication of methicillin-resistant Staphylococcus aureus in a chronic wound by a new polyhexanide hydrogel. J. Eur. Acad. Dermatol. Venereol., 2007 Nov, 21(10), 1416–7 http://dx.doi.org/10.1111/j.1468-3083.2007.02217.x[Crossref]
• [21] Daeschlein G., Assadian O., Bruck JC., Meinl C., Kramer A., Koch Feasibility and clinical applicability of polihexanide for treatment of second-degree burn wounds, SSkin. Pharmacol. Physiol., 2007, 20(6), 292–296 http://dx.doi.org/10.1159/000107577[Crossref][WoS]
• [22] Burks RI., Povidone-iodine solution in wound treatment, Phys. Ther., 1998, 78, 212–218 [PubMed]
• [23] Harvey SC., Antiseptics and disinfectants, In, Gilman AG., Goodman LS., Rall TW., Murad F., eds. The pharmacological basis of therapeutics, 7th ed. New York, Macmillian Publishing Co. Inc. 1985, 959–973
• [24] LaRocca R., LaRocca MAK., Ansell JM., Microbiology of povidoneiodine, an oveniew. In, Digenis GA., Ansell JM., eds. Proceedings of the Intemational Symposium on Povidone. Lexington, Ky, College of Pharmacy, University of Kentucky, 1983, 101–119
• [25] Anderson RL., Iodophor antiseptics, intrinsic microbial contamination with resident bacteria, Infect. Control. Hosp. Epidemiol., 1989, 10, 443–446 http://dx.doi.org/10.1086/645918[Crossref]

Identyfikatory

DOI

10.2478/s11536-008-0042-x