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2007 | 2 | 3 | 271-279
Tytuł artykułu

Identifying the etiologic role of Parvovirus B19 in non-immune hydrops fetalis by histopathology, immunohistochemistry and nucleic acid testing: a retrospective study

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Intrauterine Parvovirus B19 infections may cause fetal anemia, non-immune hydrops fetalis or abortion. This study focuses on the pathogenic role of Parvovirus B19 in non-immune hydrops fetalis at Hacettepe University, a major reference hospital in Turkey. Twenty-two cases of non-immune hydrops fetalis were retrospectively selected out of a total of 431 hydrops fetalis specimens from the Department of Pathology archieves. Paraffine embedded tissue sections from placental and liver tissues from each case were evaluated by histopathology, immunohistochemistry, nested PCR and commercial quantitative Real-time PCR. Viral DNA was detected in placental tissues by Real-time PCR in 2 cases (2/22, 9.1%) where histopathology also revealed changes suggestive of Parvovirus B19 infection. No significant histopathologic changes were observed for the remaining sections. Nested PCR that targets the VP1 region of the viral genome and immunohistochemistry for viral capsid antigens were negative for all cases. As a result, Parvovirus B19 is identified as the etiologic agent for the development of non-immune hydrops fetalis for 9.1% of the cases in Hacettepe University, Turkey. Real-time PCR is observed to be an effective diagnostic tool for nucleic acid detection from paraffine embedded tissues. Part of this study was presented as a poster at XIIIth International Congress of Virology, San Francisco, USA (Abstract V-572).
Wydawca

Czasopismo
Rocznik
Tom
2
Numer
3
Strony
271-279
Opis fizyczny
Daty
wydano
2007-09-01
online
2007-06-09
Twórcy
  • Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, 06100, Ankara, Turkey, ekoray@hacettepe.edu.tr
  • Faculty of Medicine, Department of Pathology, Hacettepe University, 06100, Ankara, Turkey
autor
  • Faculty of Medicine, Department of Pathology, Hacettepe University, 06100, Ankara, Turkey
autor
  • Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, 06100, Ankara, Turkey
autor
  • Faculty of Medicine, Department of Pathology, Hacettepe University, 06100, Ankara, Turkey
autor
  • Faculty of Medicine, Department of Pediatrics, Division of Clinical Genetics, Hacettepe University, 06100, Ankara, Turkey
  • Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, 06100, Ankara, Turkey
Bibliografia
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  • [23] P. Wattre, A. Dewilde, D. Subtil, L. Andreoletti and V. Thirion: “A clinical and epidemiological study of human parvovirus B19 in fetal hydrops using PCR southern blot hybridization and chemiluminescence detection”, J. Med. Virol., Vol. 54, (1998), pp. 140–144. http://dx.doi.org/10.1002/(SICI)1096-9071(199802)54:2<140::AID-JMV12>3.0.CO;2-N[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-007-0029-z
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