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2007 | 2 | 2 | 159-167
Tytuł artykułu

Enhanced vigilin and anionic trypsinogen expression in experimental chronic pancreatitis

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The molecular principles that lead to chronic pancreatitis are incompletely understood. Trypsin(ogen) plays a key role in the development of pancreatitis. Since the production of trypsin(ogen) by acinary pancreatic cells is paralleled by the expression of vigilin we hypothesised that vigilin may be involved in the onset of pancreatitis. Vigilin is a ubiquitous protein and has apparently high affinity to RNA. In the present study experimental pancreatitis was induced in male rats by a single intravenous application of dibutyltin dichloride (DBTC). Sections of rat pancreas were immunostained with an affinity-purified polyclonal antiserum against vigilin or trypsin(ogen). The changes in vigilin and trypsin(ogen) protein expression were determined by immunoblotting and subsequent sequence analysis of the amino acids. Induction of pancreatitis by DBTC caused alterations in the distribution and the amount of both vigilin and trypsin(ogen) as shown by immunohistochemical and immunoblot analysis. Furthermore we could demonstrate that anionic trypsinogen expression is up-regulated in DBTC-induced chronic pancreatitis. The obtained results suggest that vigilin as well as trypsin(ogen) are involved in the pathogenesis of pancreatitis and that the long-time DBTC-induced pancreatitis is a useful model for study of chronic pancreatitis.
Słowa kluczowe
Wydawca

Czasopismo
Rocznik
Tom
2
Numer
2
Strony
159-167
Opis fizyczny
Daty
wydano
2007-06-01
online
2007-06-01
Twórcy
  • Frauenhofer-Institute of Biomedical Engineering, Group of Cell Differentiation and Cell Technology at the University of Lübeck, 23538, Lübeck, Germany
  • Division of Gastroenterology, Department of Medicine, Medical Faculty, University Rostock, 18057, Rostock, Germany
  • Division of Gastroenterology, Department of Medicine, Medical Faculty, University Rostock, 18057, Rostock, Germany
autor
  • Frauenhofer-Institute of Biomedical Engineering, Group of Cell Differentiation and Cell Technology at the University of Lübeck, 23538, Lübeck, Germany
Bibliografia
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  • [11] S. Kügler, A. Grünweller, C. Probst, M.H.F. Klinger, P.K. Müller and C. Kruse: “Vigilin contains a functional localisation sequence and is present in both the cytoplasm and the nucleus”, FEBS Lett., Vol. 382, (1996), pp. 330–334. http://dx.doi.org/10.1016/0014-5793(96)00204-9[Crossref]
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  • [15] A. Schuh, K. Assmuth, I. Hilgendorf, P.K. Mueller and C. Kruse: “Protein synthesis of eukaryotic cells could be decreased by antisense-DNA of the multi KH domain protein vigilin”, Int. J. Mol. Med., Vol. 12, (2003), pp. 35–43.
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  • [22] H. Rinderknecht, I.G. Renner and C. Carmack: “Trypsinogen variants in pancreatic juice of healthy volunteers, chronic alcoholics and patients with pancreatitis and cancer of the pancreas”, Gut, Vol. 20, (1979), pp. 886–891.
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  • [24] Z. Kukor, M. Tòth and M. Sahin-Tòth: “Human anionic trypsinogen. Properties of autocatalytic activation and degradation and implications in pancreatic diseases”, Eur. J. Biochem., Vol. 270, (2003), pp. 2047–2058. http://dx.doi.org/10.1046/j.1432-1033.2003.03581.x[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-007-0019-1
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