PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
Czasopismo
2006 | 1 | 1 | 12-22
Tytuł artykułu

Evaluation of TV cell line viral susceptibility using conventional cell culture techniques

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Despite the fact that a lot of methods have been developed for rapid virus detection, classic cell culture is still “the golden standard”. The range of viruses that can be isolated and cultured in cell line systems is often limited by the susceptibility of cells to support viral replication. Since the primary cell culture, the best cellular system available to support replication of a large number of viruses, is very expensive and diffcult to obtain, cell lines, which are easier to manipulate, are commonly used for virus growth and isolation. In two previous papers we described the TV cell line initiated by our team from a laryngeal tumor, which harbors human papillomavirus (HPV) gene sequences. In this paper we analyze its capacity to support virus replication. Depending on the virus, different cytopathic effects were produced. Comparison of viral effect observed on this cell line with the effect obtained on other cell lines has been performed. This cell line might be used in the clinical virology laboratory for virus isolation.
Wydawca

Czasopismo
Rocznik
Tom
1
Numer
1
Strony
12-22
Opis fizyczny
Daty
wydano
2006-03-01
online
2006-03-01
Twórcy
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
autor
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
autor
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
Bibliografia
  • [1] Mutiu, I. Alexiu, M. Chivu, M. Petica, G. Anton, C. Bleotu, C.C. Diaconu, C. Popescu, V. Jucu and C. Cernescu: “Detection of human papillomavirus gene sequences in cell lines derived from laryngeal tumours”, J. Cell. Mol. Med. Vol. 5, (2001), pp. 49–59.
  • [2] C.C. Diaconu, C. Bleotu, M. Chivu, I. Alexiu, D. Petrusca, G. Anton, R. Achim, S.M. Ruta and C.C. Cernescu: “The development of larger cells that spontaneously escape senescence - a step during the immortalization of a human cancer cell line”, J. Cell. Mol. Med., Vol. 8, (2004), pp. 93–101.
  • [3] C.E. Cernescu and N. Cajal: “Virus - host relation”, In: Medical virology monography Medical Press, Bucharest, 1990.
  • [4] F. Kobune, H. Sakata and A. Sugiura: “Marmoset lymphoblastoid cell as a sensitive host for isolation of measles virus”, J. Virol., Vol. 64, (1990), pp. 700–705.
  • [5] J.F. Enders and T.C. Peebles: “Propagation in tissue culture of cytopathic agents from patients with measles”, Proc. Soc. Exp. Biol. Med., Vol. 86, (1954), pp. 277–286.
  • [6] K. Yamanouchi, Y. Egashira, N. Uchida, H. Kodama, F. Kobune, M. Hayami, A. Fukuda and A. Shishido: “Giant cell formation in lymphoid tissue of monkeys inoculated with various strains of measles virus”, Jpn. J. Med. Sci. Biol., Vol. 23, (1970), pp. 131–145.
  • [7] J.F. Enders, S.L. Katz and M.V. Milovanovic: “Studies of an attenuated measles virus vaccine. I. Development and preparation of the vaccine: technics for assay of effects of vaccination”, N. Engl. J. Med. Vol. 263, (1960), pp. 153–159. http://dx.doi.org/10.1056/NEJM196007282630401[Crossref]
  • [8] G.J.J. Van Doornum and J.C. De Jong: “Rapid shell vial culture tehnique for detection of enteroviruses and adenoviruses in fecal specimens: comparison with conventional virus isolation method”, J. Clin. Microbiol. Vol. 36, (1998), pp. 2865–2868.
  • [9] S.M. Lipson, K. David, F. Shaikh and L. Qian: “Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection”, JCM Vol. 39, (2001), pp. 2755–2759. [PubMed]
  • [10] K.A. Reynolds, C.P. Gerba and I.L. Pepper: “Detection of infectious enteroviruses by an integrated cell culture-pcr procedure”, Appl. Environ. Microbiol., Vol. 62, (1996), pp. 1424–1427.
  • [11] J. Ma, C.P. Gerba and I.L. Pepper: “Increased sensitivity of poliovirus detection in tap water concentrates by reverse transcriptase-polymerase chain reaction”, J. Virol. Methods Vol. 55, (1995), pp. 295–302. http://dx.doi.org/10.1016/0166-0934(95)00065-6[Crossref]
  • [12] C.J. Chapron, N.A. Ballester, J.H. Fontaine, C.N. Frades and A.B. Margolin: “Detection of astroviruses,enteroviruses and adenovirus types 409 and 41 in surface waters collected and evaluated by the information collection rule and an integrated cell culture-nested PCR procedure”, Appl. Envirom. Microbiol., Vol. 66, (2000), pp. 2520–2525. http://dx.doi.org/10.1128/AEM.66.6.2520-2525.2000[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-006-0007-x
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.