SIL-based confocal fluorescence microscope for investigating individual nanostructures

• Autorzy: Bartosz Krajnik , Tim Schulte , Dawid Piątkowski , Nikodem Czechowski , Eckhard Hofmann , Sebastian Mackowski
• Języki publikacji: EN

Abstrakty

EN

We developed a fluorescence confocal microscope equipped with a hemispherical solid immersion lens (SIL) and apply it to study the optical properties of light-harvesting complexes. We demonstrate that the collection efficiency of the SIL-equipped microscope is significantly improved, as is the spatial resolution, which reaches 600 nm. This experimental setup is suitable for detailed studies of physical phenomena in hybrid nanostructures. In particular, we compare the results of fluorescence intensity measurements for a light-harvesting peridinin-chlorophyll-protein (PCP) complex with and without the SIL.

Słowa kluczowe

EN

confocal microscopy; light-harvesting complex; solid immersion lens; fluorescence imaging

• Czasopismo: Open Physics
• Rocznik: 2011
• Tom: 9
• Numer: 2
• Strony: 293-299

Daty

wydano

2011-04-01

online

2011-02-20

Twórcy

autor

Bartosz Krajnik

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland,

autor

Tim Schulte

• Department of Biology and Biotechnology, Ruhr-University Bochum, Bochum, Germany

autor

Dawid Piątkowski

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland

autor

Nikodem Czechowski

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland

autor

Eckhard Hofmann

• Department of Biology and Biotechnology, Ruhr-University Bochum, Bochum, Germany

autor

Sebastian Mackowski

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland,

Bibliografia

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Identyfikatory

DOI

10.2478/s11534-010-0098-5