Development of a nanocomposite system and its application in biosensors construction

• Autorzy: Ramona Penu , Ioana Vasilescu , Sandra Eremia , Florentina Gatea , Gabriel-Lucian Radu , Simona-Carmen Litescu
• Języki publikacji: EN

Abstrakty

EN

The present work describes the development of a nanocomposite system and its application in construction of a new amperometric biosensor applied in the determination of total polyphenolic content from propolis extracts. The nanocomposite system was based on covalent immobilization of laccase on functionalized indium tin oxide nanoparticles and it was morphologically and structural characterized. The casting of the developed nanocomposite system on the surface of a screen-printed electrode was used for biosensor fabrication. The analytical performance characteristics of the settled biosensor were determined for rosmarinic acid, caffeic acid and catechol (as laccase specific substrate). The linearity was obtained in the range of 1.06×10−6 − 1.50×10−5 mol L−1 for rosmarinic acid, 1.90×10−7 − 2.80×10−6 mol L−1 for caffeic acid and 1.66×10−6 − 7.00×10−6 mol L−1 for catechol. A good sensitivity of amperometric biosensor 141.15 nA µmol−1 L−1 and fair detection limit 7.08×10−8 mol L−1 were obtained for caffeic acid. The results obtained for polyphenolic content of propolis extracts were compared with the chromatographic data obtained by liquid-chromatography with diode array detection. [...]

Słowa kluczowe

EN

Nanocomposite; Laccase biosensor; Polyphenols; Propolis extracts; Indium tin oxide nanoparticles

• Czasopismo: Open Chemistry
• Rocznik: 2013
• Tom: 11
• Numer: 6
• Strony: 968-978

Daty

wydano

2013-06-01

online

2013-03-28

Twórcy

autor

Ramona Penu

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

autor

Ioana Vasilescu

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

autor

Sandra Eremia

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

autor

Florentina Gatea

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

autor

Gabriel-Lucian Radu

• Faculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, Bucharest, 0101061, Romania,

autor

Simona-Carmen Litescu

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

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Identyfikatory

DOI

10.2478/s11532-013-0222-7