Application of UV detection in HPLC in the total antioxidant potential assay

• Autorzy: Paweł Wantusiak , Bronisław Głód
• Języki publikacji: EN

Abstrakty

EN

HPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products. [...]

Słowa kluczowe

EN

Total antioxidant potential; HPLC; UV detection; Honey; Fenton reaction

• Czasopismo: Open Chemistry
• Rocznik: 2012
• Tom: 10
• Numer: 6
• Strony: 1786-1790

Daty

wydano

2012-12-01

online

2012-09-21

Twórcy

autor

Paweł Wantusiak

• Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, University of Natural Sciences and Humanities, 08-110, Siedlce, Poland

autor

Bronisław Głód

• Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, University of Natural Sciences and Humanities, 08-110, Siedlce, Poland,

Bibliografia

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Identyfikatory

DOI

10.2478/s11532-012-0108-0