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2012 | 10 | 4 | 1073-1078
Tytuł artykułu

Impact of un-polymerized acrylamide monomer residues onto protein identification by MALDI TOF MS

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Polyacrylamide gel electrophoresis is a powerful tool for protein mixture separation frequently used in proteomic studies. This article describes the problems which can arise during the identification process of separated proteins by matrix assisted laser desorption/ionization mass spectrometry. Presence of residual acrylamide monomers in the peptide samples leads to a significant peptide signal suppression. In the case of protein prohibitin, isolated from the MCF 7 breast cancer cell line, the peptide signal suppression was observed in 50% of all detected peptides. [...]
Wydawca

Czasopismo
Rocznik
Tom
10
Numer
4
Strony
1073-1078
Opis fizyczny
Daty
wydano
2012-08-01
online
2012-05-29
Twórcy
autor
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia, ivan.talian@upjs.sk
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
autor
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11532-012-0024-3
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