Czasopismo
Tytuł artykułu
Warianty tytułu
Języki publikacji
Abstrakty
A reversed-phase high-performance liquid chromatographic (HPLC) method is applied for the determination of galacturonic acid (GA) of pectins in different commercial fruit juices. The separation was carried out on a C18 column using precolumn derivatization with p-aminobenzoic acid (p-ABA) and UV detection at 304 nm. The identification of GA was confirmed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in positive ion mode. The concentration of GA in the samples analyzed ranged from 12.9 ± 0.5 to 49.4 ± 0.5 mgGA L−1. Amongst the samples analyzed, mango juice was found to be richest in GA content, and therefore a good source of pectins. Detection and quantification limits of the described methodology were 1.2 and 3.9 mg L−1, respectively. Quantitative GA recoveries in the beverages had a range between 90 and 98%. The results showed that the HPLC method proposed was precise and suitable for the identification and quantification of GA in commercial fruit juices.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Numer
Strony
1236-1243
Opis fizyczny
Daty
wydano
2010-12-01
online
2010-10-08
Twórcy
autor
- REQUIMTE — Chemistry and Biochemistry Department, Faculty of Science, University of Porto, 4169-007, Porto, Portugal
autor
- REQUIMTE — Chemistry and Biochemistry Department, Faculty of Science, University of Porto, 4169-007, Porto, Portugal, lfguido@fc.up.pt
autor
- iBeSa — The Beverages and Health Institute, Apartado 1044, 4466-955, S. Mamede de Infesta, Portugal
autor
- REQUIMTE — Chemistry and Biochemistry Department, Faculty of Science, University of Porto, 4169-007, Porto, Portugal
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11532-010-0101-4