PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
Czasopismo
2010 | 8 | 4 | 732-736
Tytuł artykułu

Detection of short oligonucleotide sequences using an electrochemical DNA hybridization biosensor

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
An electrochemical DNA hybridization biosensor was developed for the detection of DNA hybridization using MDB and proflavine as electrochemical labels. The biosensor was based on the interaction of 7-dimethyl-amino-1,2-benzophenoxazi-nium Meldola’s Blue (MDB) and proflavine with double stranded DNA (dsDNA) The electrochemical behaviour of MDB and proflavine as well as its interaction with double stranded (dsDNA) were investigated by cyclic (CV) and square wave voltammetry (SWV) and screen printed electrodes (ScPE). Furthermore, DNA-hybridization biosensors were developed for the detection of hybridization between oligonucleotides, which was detected by studying changes in the voltammetric peaks of MDB (reduction peak at −0.251 V) and proflavine (reduction peak at 0.075 V). MDB and proflavine were found to intercalate between the base pairs of dsDNA and oligonucleotides. Several factors affecting the dsDNA or oligonucleotides immobilization, hybridization and indicator preconcentration and interaction time, were investigated. As a result of the interaction of MDB with dsDNA and hybridized oligonucleotides, the voltammetric signals of MDB increased. Furthermore, guanine’s oxidation peak (at 0.901 V) was decreased as MDB’s concentration was increased. As a result of the interaction of proflavine with dsDNA and hybridized oligonucleotides, the voltammetric signals of proflavine decreased. These results were similar for carbon paste and screen printed electrodes. A comparison of the performance between CPE and ScPE was done. Our results showed that lower concentrations of MDB and proflavine were detected using screen printed electrodes. Moreover, reproducibility was better using screen printed electrodes and the detection was faster (regarding the experimental steps), but they are more cost effective. [...]
Wydawca

Czasopismo
Rocznik
Tom
8
Numer
4
Strony
732-736
Opis fizyczny
Daty
wydano
2010-08-01
online
2010-06-17
Twórcy
autor
  • Analytical Chemistry Laboratory, Chemistry Department, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece, girousi@chem.auth.gr
  • Analytical Chemistry Laboratory, Chemistry Department, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
Bibliografia
  • [1] A.K. Bej, Nucleic Acid Analysis: Principles and Bioapplications (Wiley-Liss Press, New York, 1996) Chapter 1
  • [2] J. Hodgson, Nature Biotech. 16, 725 (1998) http://dx.doi.org/10.1038/nbt0898-725[Crossref]
  • [3] K. Millan et al., Anal. Chem. 66, 2943 (1994) http://dx.doi.org/10.1021/ac00090a023[Crossref]
  • [4] J. Wang et al., Anal. Chim. Acta 326, 141 (1996) http://dx.doi.org/10.1016/0003-2670(96)00042-6[Crossref]
  • [5] J. Wang et al., Anal. Chim. Acta 337, 41 (1997) http://dx.doi.org/10.1016/S0003-2670(96)00395-9[Crossref]
  • [6] J. Wang et al., Electroanal. 9, 395 (1997) http://dx.doi.org/10.1002/elan.1140090508[Crossref]
  • [7] E. Palecek et al., Electroanal. 9, 990 (1997) http://dx.doi.org/10.1002/elan.1140091305[Crossref]
  • [8] J. Liu et al. Electroanal. 8, 803 (1996) http://dx.doi.org/10.1002/elan.1140080818[Crossref]
  • [9] S. Liu et al., Anal. Chim. Acta 335, 239 (1996) http://dx.doi.org/10.1016/S0003-2670(96)00331-5[Crossref]
  • [10] M. Fojta et al., Electroanal. 8, 420 (1996) http://dx.doi.org/10.1002/elan.1140080504[Crossref]
  • [11] A. Erdem, B. Meric, K. Kerman, T. Dalbasti, M. Ozsoz, Electroanal. 11, 1372 (1999) http://dx.doi.org/10.1002/(SICI)1521-4109(199912)11:18<1372::AID-ELAN1372>3.0.CO;2-4[Crossref]
  • [12] A. Erdem et al., Electroanal. 10, 586 (1999) http://dx.doi.org/10.1002/(SICI)1521-4109(199906)11:8<586::AID-ELAN586>3.0.CO;2-J[Crossref]
  • [13] K.M. Millan et al., Anal. Chem. 65, 2317 (1993) http://dx.doi.org/10.1021/ac00065a025[Crossref]
  • [14] K.M. Millan et al., Electroanalysis 4, 929 (1992) http://dx.doi.org/10.1002/elan.1140041003[Crossref]
  • [15] J. Wang et al., Anal. Chem. 68, 2629 (1996) http://dx.doi.org/10.1021/ac9602433[Crossref]
  • [16] J. Wang et al., Anal. Chem. 70, 3699 (1998) http://dx.doi.org/10.1021/ac980092z[Crossref]
  • [17] K. Kerman et al., Turk. J. Chem. 28, 523 (2004)
  • [18] K. Kerman et al., Sci. Tech. Adv. Mat, 5, 351 (2004) http://dx.doi.org/10.1016/j.stam.2004.01.009[Crossref]
  • [19] S. Dasgupta et al., Biochim. Biophys. Acta 264, 38 (1973)
  • [20] M. Vanickova, Collect. Czech. Chem. Commun. 65 (2000) [Crossref]
  • [21] M. Aslanoglu, Anal. Sci. 22 (2006) [Crossref]
  • [22] S. Girousi, D. Alexiadou, A. Ioannou, Microchim. Acta 160, 435 (2008) http://dx.doi.org/10.1007/s00604-007-0812-1[Crossref]
  • [23] P. Kara, B. Meric, A. Zeytinoglu, M. Ozsoz, Analytica Chimica Acta 518, 69 (2004) http://dx.doi.org/10.1016/j.aca.2004.04.004[Crossref]
  • [24] D. Alexiadou, A. Ioannou, S. Kouidou-Andreou, A. Voulgaropoulos, S. Girousi, Anal. Lett. 41, 1742 (2008) http://dx.doi.org/10.1080/00032710802162335[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11532-010-0056-5
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.