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2010 | 8 | 1 | 87-95
Tytuł artykułu

Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The analytical method based on the high-performance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL−1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43% of DPPH radical inhibition for PFE method and from 76.01 to 89.94% in the case of USE method. [...]
Wydawca

Czasopismo
Rocznik
Tom
8
Numer
1
Strony
87-95
Opis fizyczny
Daty
wydano
2010-02-01
online
2010-02-16
Twórcy
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
autor
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic, Martin.Adam@upce.cz
autor
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11532-009-0125-9
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