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2009 | 7 | 4 | 787-793
Tytuł artykułu

Activity and kinetics studies of yeast alcohol dehydrogenase in a reverse micelle formulated from functional surfactants

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Yeast alcohol dehydrogenase (YADH) showed substantial decrease in its catalytic activity due to the strong electrostatic interaction between the head groups of sodium bis(2-ethylhexyl) sulfosuccinate (AOT) and YADH in AOT reverse micelles. However, the catalytic activity of YADH in a nonionic reverse micellar interface (GGDE/TX-100) obtained from a functional nonionic surfactant N-gluconyl glutamic acid didecyl ester (GGDE) and Triton X-100 (TX-100) was higher than that in AOT reverse micelle under the respective optimum conditions. A comparison of the kinetic parameters showed that the turnover number kcat in GGDE/TX-100 reverse micelle was 1.4 times as large as that in AOT reverse micelle, but the Michaelis constants in AOT reverse micelle for ethanol K mB was twice and for coenzyme NAD+ K mA was 5 times higher than their counterparts in GGDE/TX-100 reverse micelle. For the conversion of ethanol, the smaller K mB and larger kcat in GGDE/TX-100 reverse micelle resulted in higher catalytic efficiency kcat/K mB. The stability of YADH in GGDE/TX-100 reverse micelle was also found to be better than that in AOT reverse micelle. They were mainly attributed to the absence of electric charge on the head groups of GGDE and TX-100 in the GGDE/TX-100 reverse micelle. [...]
Wydawca

Czasopismo
Rocznik
Tom
7
Numer
4
Strony
787-793
Opis fizyczny
Daty
wydano
2009-12-01
online
2009-10-06
Twórcy
autor
  • Key Laboratory of Colloid & Interface Chemistry of the Education Ministry of China, Shandong University, Jinan, 250100, China
autor
  • Key Laboratory of Colloid & Interface Chemistry of the Education Ministry of China, Shandong University, Jinan, 250100, China
autor
  • Key Laboratory of Colloid & Interface Chemistry of the Education Ministry of China, Shandong University, Jinan, 250100, China
autor
  • State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, China
autor
  • State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, China
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11532-009-0069-0
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