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Czasopismo
2009 | 7 | 3 | 532-541
Tytuł artykułu

Polycationic graft copolymers of poly(N-vinylpyrrolidone) as non-viral vectors for gene transfection

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Novel graft copolymers of 2-(dimethylamino)ethyl methacrylate (DMAEMA) with N-vinylpyrrolidone (NVP) were designed and synthesized by the free radical copolymerization of DMAEMA with precursor polymers of vinyl-functionalized poly(N-vinylpyrrolidone) (PVP). The ability of the PVP- grafted copolymers to bind and condense DNA was confirmed by ethidium bromide displacement assay, agarose gel electrophoresis and transmission electron microscopy. The presence of PVP in the copolymers had a favorable effect on the biophysical properties of polymer/DNA complexes. Colloidal stable complexes obtained from the copolymer systems, were shown to be separate, uniformly spherical nanoparticles by transmission electron microscopy. The approximate diameter of the complexes was 150–200 nm, as determined by dynamic light scattering studies. These results confirm an important role played by the PVP grafts in producing compact stable DNA complexes. The ζ-potential measurements revealed that the incorporation of the PVP grafts reduced the positive surface charge of polymer/DNA complexes. The cytotoxicity of the copolymers decreased with an increasing fraction of PVP. Furthermore, in vitro transfection experiments with these copolymers showed improved ability of transfection in cell culture, demonstrating an important role for PVP grafts in enhancement of the transfection efficiency. [...]
Wydawca

Czasopismo
Rocznik
Tom
7
Numer
3
Strony
532-541
Opis fizyczny
Daty
wydano
2009-09-01
online
2009-06-21
Twórcy
autor
  • Department of Applied Chemistry, East China Institute of Technology, Fu Zhou, 344000, PR China, walton_liu@163.com
  • Department of Applied Chemistry, East China Institute of Technology, Fu Zhou, 344000, PR China
  • Department of Applied Chemistry, East China Institute of Technology, Fu Zhou, 344000, PR China
autor
  • Department of Applied Chemistry, East China Institute of Technology, Fu Zhou, 344000, PR China
Bibliografia
  • [1] K.A. Partridge, R.O.C. Oreffo, Tissue. Eng. 10, 295 (2004) http://dx.doi.org/10.1089/107632704322791934[Crossref]
  • [2] M. Haider, Z. Megeed, H. Ghandehari, J. Control. Rel. 95, 1(2004) http://dx.doi.org/10.1016/j.jconrel.2003.11.011[Crossref]
  • [3] P. Lehn, S. Fabrega, N. Oudrhiri, J. Navarro, Adv. Drug Delivery Rev. 30, 5 (1998) http://dx.doi.org/10.1016/S0169-409X(97)00102-6[Crossref]
  • [4] G.D. Schmidt-Wolf, I.G.H. Schmidt-Wolf, Trends. Mol. Med. 9, 67 (2003) http://dx.doi.org/10.1016/S1471-4914(03)00005-4[Crossref]
  • [5] S. Han, R.I. Mahato, Y.K. Sung, S.W. Kim, Mol. Therapy 2, 302 (2000) http://dx.doi.org/10.1006/mthe.2000.0142[Crossref]
  • [6] T.G. Park, J.H. Jeong, S.W. Kim, Adv. Drug Delivery Rev. 58, 467 (2006) http://dx.doi.org/10.1016/j.addr.2006.03.007[Crossref]
  • [7] A.V. Slita, N.A. Kasyanenko, O.V. Nazarova, I.I. Gavrilova, E.M. Eropkina, A.K. Sirotkin, T.D. Smirnova, O.I. Kiselev, E.F. Pannarin, J. Biotech. 127, 679 (2007) http://dx.doi.org/10.1016/j.jbiotec.2006.07.016[Crossref]
  • [8] F. Liu, L. Huang, J. Control. Rel. 78, 259 (2002) http://dx.doi.org/10.1016/S0168-3659(01)00494-1[Crossref]
  • [9] A.G. Schatzlein, Anti-Cancer Drugs 12, 275 (2001) http://dx.doi.org/10.1097/00001813-200104000-00001[Crossref]
  • [10] A.C. Hunter, Adv. Drug Delivery Rev. 58, 1523 (2006) http://dx.doi.org/10.1016/j.addr.2006.09.008[Crossref]
  • [11] G.A. Pietersz, C.-K. Tang, V. Apostolopoulos, Mini-Rev. Med. Chem. 6, 1285 (2006) http://dx.doi.org/10.2174/138955706778992987[Crossref]
  • [12] H.-L. Jiang, Y.K. Kim, R. Arote, J.W. Nah, M.H. Cho, Y.J. Choi, T. Akaike, C.S. Cho, J. Control. Rel. 117, 273 (2007) http://dx.doi.org/10.1016/j.jconrel.2006.10.025[Crossref]
  • [13] S.-F. Li, W. Dong, Y.-W. Zong, W. Yin, G.-H. Jin, Q.-G. Hu, X.-F. Huang, W.-H. Jiang, Z.-C. Hua, Mol. Ther. 15, 515 (2007) http://dx.doi.org/10.1038/sj.mt.6300072[Crossref]
  • [14] G. Minigo, A. Scholzen, C.-K. Tang, J.C. Hanley, M. Kalkanidis, G.A. Pietersz, V. Apostolopoulos, M. Plebanski, Vaccine 25, 1316 (2007) http://dx.doi.org/10.1016/j.vaccine.2006.09.086[Crossref]
  • [15] D.S. Shah, T. Sakthivel, I. Toth, A.T. Florence, A.D. Wilderspin, Int. J. Pharm. 208, 41 (2000) http://dx.doi.org/10.1016/S0378-5173(00)00534-2[Crossref]
  • [16] Y. Onishi, Y. Eshita, A. Murashita, M. Mizuno, J. Yoshida, Nanomedicine 3, 184 (2007) [Crossref]
  • [17] M. Hashimoto, M. Morimoto, H. Saimoto, Y. Shigemasa, H. Yanagie, M. Eriguchi, T. Sato, Biotech. Letters 28, 815 (2006) http://dx.doi.org/10.1007/s10529-006-9006-x[Crossref]
  • [18] P. van de Wetering, N.M.E. Schuurmans-Nieuwenbroek, M.J. van Streenbergen, D.J.A. Crommelin, W.E. Hennink, J. Control. Rel. 64, 193 (2000) http://dx.doi.org/10.1016/S0168-3659(99)00130-3[Crossref]
  • [19] M.A. Wolfert, P.R. Dash, O. Nazarova, D. Oupicky, L.W. Seymour, S. Smart, J. Strohalm, K. Ulbrich, Bioconjug. Chem. 10, 993 (1999) http://dx.doi.org/10.1021/bc990025r[Crossref]
  • [20] P. van de Wetering, J.Y. Cherng, H. Talsma, W.E. Hennink, J. Control. Rel. 49, 59 (1997) http://dx.doi.org/10.1016/S0168-3659(97)00059-X[Crossref]
  • [21] M.-X. Tang, F.C. Szoka, Gene Ther. 4, 823 (1997) http://dx.doi.org/10.1038/sj.gt.3300454[Crossref]
  • [22] J.-F. Tan, H.P. Too, T.A. Hatton, K.C. Tam, Langmuir 22, 3744 (2006) http://dx.doi.org/10.1021/la052591i[Crossref]
  • [23] M.A. Wolfert, E.H. Schacht, V. Toncheva, K. Ulbrich, O. Nazarova, L.W. Seymour, Hum. Gene Ther. 7, 2123 (1996) http://dx.doi.org/10.1089/hum.1996.7.17-2123[Crossref]
  • [24] U. Rungsardthong, M. Deshpande, L. Bailey, M. Vamvakaki, S.P. Armes, M.C. Garmett, S. Stolnik, J. Control. Rel. 73, 359 (2001) http://dx.doi.org/10.1016/S0168-3659(01)00295-4[Crossref]
  • [25] V. Toncheva, M.A. Wolfert, P.R. Dash, D. Oupicky, K. Ulbrich, L.W. Seymour, Biochim. Biophys. Acta 1380, 354 (1998)
  • [26] J.-F. Tan, P. Ravi, H.P. Too, T.A. Hatton, K.C. Tam, Biomacromolecules 6, 498 (2005) http://dx.doi.org/10.1021/bm049426m[Crossref]
  • [27] L. Bromberg, S. Deshmukh, M. Temchenko, L. Iourtchenko, V. Alakhov, C. Alvarez-Lorenzo, R. Barreiro-Iglesisa, A. Concheiro, T.A. Hatton, Bioconjugate Chem. 16, 626 (2005) http://dx.doi.org/10.1021/bc049749f[Crossref]
  • [28] C.K. Nisha, S.V. Manorama, M. Ganguli, S. Maiti, J.N. Kizhakkedathu, Langmuir 20, 2386 (2004) http://dx.doi.org/10.1021/la035737r[Crossref]
  • [29] Y.T.A. Chim, J.K.W. Lam, Y. Ma, S.P. Armes, A.L. Lewis, C.J. Roberts, S. Stolnik, S.J.B. Tendler, M.C. Davies, Langmuir 21, 3591 (2005) http://dx.doi.org/10.1021/la047480i[Crossref]
  • [30] D.W. Lim, Y.H. Yeom, T.G. Park, Bioconjugate Chem. 11, 688 (2000) http://dx.doi.org/10.1021/bc000014u[Crossref]
  • [31] Z.-Q. Wu, S. Yang, W.-Y. Liao, L.-Z. Meng, Chin. J. Polym. Sci. 24, 315 (2006) http://dx.doi.org/10.1142/S0256767906001345[Crossref]
  • [32] V.P. Torchilin, T.S. Levchenko, K.R. Whiteman, Biomaterials 22, 3035 (2001) http://dx.doi.org/10.1016/S0142-9612(01)00050-3[Crossref]
  • [33] S.C. De Smedt, J. Demeester, W.E. Hennink, Pharm. Res. 17, 113 (2000) http://dx.doi.org/10.1023/A:1007548826495[Crossref]
  • [34] P. van de Wetering, E. Moret, N.M.E. Schuurmans-Nieuwenbrock, M.J. van Streenbergen, W.E. Hennink, Bioconjugate Chem. 10, 589 (1999) http://dx.doi.org/10.1021/bc980148w[Crossref]
  • [35] H. Lodish, A. Berk, P.D.B. Matsudaira, J. Darnell, Molecular cell biology, 4th edition (WH Freeman & Company, New York, 2001)
  • [36] U. Rungsardthong, M. Deshpande, L. Bailey, M. Vamvakaki, S.P. Armes, M.C. Garnett, S. Stolnik, J. Control. Rel. 73, 359 (2001) http://dx.doi.org/10.1016/S0168-3659(01)00295-4[Crossref]
  • [37] S. Stolnik, I. Illum, S.S. Davis, Adv. Drug Del. Rev. 16, 195 (1995) http://dx.doi.org/10.1016/0169-409X(95)00025-3[Crossref]
  • [38] P. van de Watering, J.Y. Cherng, H. Talsma, D.J.A. Crommelin, W.E. Hennink, J. Control. Rel. 53, 145 (1998) http://dx.doi.org/10.1016/S0168-3659(97)00248-4[Crossref]
  • [39] U. Rungsardthong, T. Ehtezazi, L. Bailey, S.P. Armes, M.C. Garnett, S. Stolnik, Biomacromolecules 4, 683 (2003) http://dx.doi.org/10.1021/bm025736y[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11532-009-0045-8
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