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2008 | 6 | 4 | 509-512
Tytuł artykułu

Interaction of serum albumin with vinyl sulfonate azo dye

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The interaction between bovine serum albumin and the mono azo reactive dye Orange ZT has been investigated using absorption difference spectroscopy. The influence of pH and ionic strength of the solution on the stability of the dye-protein complex has been determined. At 25°C, the complex dissociation constants were equal to 24.0, 28.0, 7.0, 11.0, 17.6 and 46.0 μM at pH 7.0, 6.5, 6.0, 5.5, 5.0 and 4.3, respectively. In the presence of 0.1, 0.2, 0.3 M KCl, at pH 6.0 and 25°C, the complex dissociation constants were 8.8, 20.0, 18.0 μM, respectively. The protein-dye complex dissociation constants show that Orange ZT could be used as an affinity ligand for protein purification. [...]
Wydawca

Czasopismo
Rocznik
Tom
6
Numer
4
Strony
509-512
Opis fizyczny
Daty
wydano
2008-12-01
online
2008-10-28
Twórcy
  • Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223, Vilnius-40, Lithuania, sjolanta@fm.vgtu.lt
  • Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223, Vilnius-40, Lithuania
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11532-008-0066-8
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