Determination of phenolic acids by capillary zone electrophoresis and HPLC

• Autorzy: František Kvasnička , Jana Čopíková , Rudolf Ševčík , Jana Krátká , Andrej Syntytsia , Michal Voldřich
• Języki publikacji: EN

Abstrakty

EN

Selected phenolic acids are determined by capillary zone electrophoresis and HPLC, each using UV detection. The optimised CZE background electrolyte contained 50 mM acetic acid, 95 mM 6-aminocaproic acid, 0.1% polyacrylamide, 1% polyvinylpyrrolidone, and 10% methanol. Twelve phenolic acids (gallic, p-hydroxybenzoic, 3,4-dihydroxybenzoic, vanillic, syringic, o-coumaric, p-coumaric, caffeic, sinapic, ferulic, salicylic and chlorogenic) were separated within 10 minutes. Chromatographic separation of these phenolic acids was carried out on an Eclipse XBD C8 column using a mobile phase gradient (acetonitrile / methanol / water / 0.1% phosphoric acid); all were separated within 25 minutes. Electrophoretic and chromatographic determinations of ferulic and chlorogenic acids were compared on barley, malt, and potato samples. The methods’ characteristics were: linearity (1–20 mg ml and 0.2–4 mg ml−1), accuracy (recovery 94 ± 5% and 96 ± 4%), intra-assay repeatability (4.1% and 3.5%), and detection limit (0.2 and 0.02 mg ml−1). [...]

Słowa kluczowe

EN

Capillary electrophoresis   HPLC   Phenolic acids   Barley   Potato  

• Czasopismo: Open Chemistry
• Rocznik: 2008
• Tom: 6
• Numer: 3
• Strony: 410-418

Daty

wydano

2008-09-01

online

2008-08-11

Twórcy

autor

František Kvasnička

• Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic,

autor

Jana Čopíková

• Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic

autor

Rudolf Ševčík

• Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic

autor

Jana Krátká

• Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic

autor

Andrej Syntytsia

• Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic

autor

Michal Voldřich

• Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic

Bibliografia

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Identyfikatory

DOI

10.2478/s11532-008-0032-5