Spectroscopic methods of studying enzyme action in wool fibre

• Autorzy: Magdalena Fogorasi , Elisabeth Heine
• Języki publikacji: EN

Abstrakty

EN

Enzymes may be used to develop an environmentally friendly alternative to the conventional polluting technologies in textile finishing. The action of the unlabeled and fluorescent labeled proteolytic enzymes subtilisin and trypsin on wool was examined. Scanning electron micrographs and a diffusion study, based on fluorescence microscopy, localized the enzymatic attack on the fiber. A kinetic study was carried out by monitoring the amino acid content of the treatment liquor. Enzymatic action is not confined to the fiber surface. To limit attack to the surface and reduce wool damage new treatment methods such as enzyme immobilization onto a solid carrier must be investigated.

Słowa kluczowe

EN

Proteolytic activity; diffusion; kinetic measurements; fluorescence microscopy; wool; subtilisin; trypsin; fiber finishing

• Czasopismo: Open Chemistry
• Rocznik: 2006
• Tom: 4
• Numer: 4
• Strony: 786-797

Daty

wydano

2006-12-01

online

2006-12-01

Twórcy

autor

Magdalena Fogorasi

• Textile Department, Aurel Vlaicu University, 310130, Arad, Romania,

autor

Elisabeth Heine

• Deutsches Wollforschungsinstitut an der RWTH Aachen e. V., D-52056, Aachen, Germany

Bibliografia

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• [7] E. Heine: Grundlegende Untersuchungen zur Einwirkung von Enzymen auf Wolle, Thesis (PhD), Technische Hochschule Aachen, 1991.
• [8] M.S. Fogorasi: Ecological Finishing Procedures obtained by means of Biochemical Change of Cellulosic and Proteic Fibers, Thesis (PhD), Polytechnical Institute “Gh. Asachi” Isai, 2003.
• [9] M.S. Fogorasi, A. Grigoriu and E. Heine: “Immobilized Enzymes and their Action on Wool Fibers”, Bulletin of Polytechnical Institute of Iasi, XLVIII(LII), Fasc. 1–2, Textile-Leather, (2002), pp. 59–67.
• [10] C. Silva: “Enzymatic Treatment of Wool with Modified Proteases”, Thesis (PhD), Universidada do Minho, 2005.

Identyfikatory

DOI

10.2478/s11532-006-0045-x