PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 85 | 3 | 114-122
Tytuł artykułu

Morphological changes of the pancreas in course of acute pancreatitis during treatment with Ulinastatin

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Acute pancreatitis is a severe clinical conditio that causes significant mortality in patients. Since we do not have at the moment effective causal treatment research on the use of pro tease inhibitors can produce tangible benefits. In view of the growing number of cases and high mortality in severe AP with one hand, and the lack of a usal treatment research efforts undertaken to search for effective drugs for this disease seem to have deep reasons. Aim of the study was to determine the histopathological changes in the pancreas in the treatment of acute pancreatitis with Ulinastatin. Material and methods. The study was conducted in male Wistar rats weighing 250-300 grams. 150 individuals were used for the experiment, 60 of them were treated with Ulinastatin. Experimental acute pancreatitis was induced by the model proposed by Aho and Henckel using sodium taurocholate. Ulinastatin dose numer depended on the duration of the experiment. For histopathological examination pancreatic fragments weighing approximately 1 g each were taken. Assessment and documentation of histopathological preparations were made by light microscopy. Results. Evaluation of the histological preparations of various time groups showed significantly improved results after application of Ulinastatin, depending on the duration of the inflammation and the number of doses of the drug. Conclusions. Application for the treatment of UTI leads to inhibition of the inflammatory process at the stage of pancreatic edema and in cases of severe necrotizing course limits the progression of the disease which gives grounds for its clinical use in humans.
Słowa kluczowe
Wydawca

Rocznik
Tom
85
Numer
3
Strony
114-122
Opis fizyczny
Daty
wydano
2013-03-01
online
2013-04-20
Twórcy
  • 2nd Department and Clinic of General, Gastroenterologic Surgery and Tumours of Alimentary Tract, Medical University in Lublin Kierownik: prof. dr hab. G. Wallner
  • 2nd Department and Clinic of General, Gastroenterologic Surgery and Tumours of Alimentary Tract, Medical University in Lublin Kierownik: prof. dr hab. G. Wallner
  • Department of General and Oncologic Surgery Fryderyk Chopin Regional Specialist Hospital in Rzeszów Ordynator: dr n. med. A. Surowiec
  • 2nd Department and Clinic of General, Gastroenterologic Surgery and Tumours of Alimentary Tract, Medical University in Lublin Kierownik: prof. dr hab. G. Wallner
  • Department of Orthopedics and Rehabilitation Medical University in Lublin Kierownik: prof. dr hab. M. Jabłoński
  • 2nd Department and Clinic of General, Gastroenterologic Surgery and Tumours of Alimentary Tract, Medical University in Lublin Kierownik: prof. dr hab. G. Wallner
Bibliografia
  • 1. Gabryelewicz A, Rydzewska G: Wybrane zagadnienia z patofizjologii trzustki. W : Chirurgia trzustki.(W Kozuschek red.) PZWL, Warszawa 1999; 49-66.
  • 2. Beger HG, Rau B, Mayer J et al.: Natural course of acute pancreatitis. W J Sur. 1997; 21: 130-35.
  • 3. Puchalski Z, Ładny JR: Chirurgia trzustki. O Chirurgii Polskiej końca XX wieku. Fundacja Polski Przegląd Chirurgiczny 2001; 312-16.
  • 4. Gabryelewicz A, Puchalski Z: Leczenie ostrego zapalenia trzustki. Pol Przegl Chir 1993; 65: 607-12.
  • 5. Mayer KL , Ho HS , Frey CF : Acute pancreatitis, in John L. Cameron, ed: Current surgical therapy. 6th ed, St. Louis, Mosby, 1998; 487-93.
  • 6. Kobayashi H, Hirashima Y, Sun GW et al.: Identification and characterization of the cell-associated binding protein for urinary trypsin inhibitor. BiochemBiophys Acta 1998; 1383: 253-68.
  • 7. Tani O, Otsuki M, Itoh H et al.: The protective effect of the trypsin inhibitor Urinastatin on cerulein- induced acute pancreatitis in rats. Panceas 1988; 3; 471-76.[Crossref]
  • 8. Aho HJ, Konkenssalo ML , Nevalainen TL : Experimental pancreatitis in the rats. Sodium taurocholane induced acute haemorrhagic pancreatitis. Scand J Gastroenterol 1980; 15: 411-16.[Crossref][PubMed]
  • 9. Aho HJ. Nevalainen TJ: Experimental pancreatitis in the rat. Ultrastructure of Sodium Taurocholane- induced pancreatic lesions. Scand J Gastroenterol 1980; 15: 417-24.[Crossref][PubMed]
  • 10. Aho HJ, Nevelainen TJ, Aho AJ: Experimental pancreatitis in the rat. Development of pancreatic necrosis, ischemia, edema after inductal sodium taurocholane injection. Eur Surg Res 1983; 15: 28-36.[PubMed][Crossref]
  • 11. Hara A, Ito T et al.: A case of severe acute pancreatitis succesfuully treated by continuous arterial infusion of a protease inhibitor. Nippon ShokakibyoGakkai Zasshi 1994; 91: 2021-25.
  • 12. Heinkel K: Die ratte als Versuchstier in der Experimentallen Pankreasdiagnostik. Klin Wochenscher 1953; 31: 815-21.[Crossref]
  • 13. Spormann H, Sokołowski A, Letko G: Experimental acute pancreatitis - a quantification of dynamics at enzymie and histologic levels. PathRes Pract 1989; 85: 358-62.
  • 14. S pormann H. Sokołowski A,Letko G: Exparimental acute pancreatitis-a qualification of dynamics et enzymie and histomorphologic levels. PatholRes Prat 1989; 185: 507-12.
  • 15. A riada T, Fry CF , Ruebner B et al.: Therapeutic regiments in acute experimental pancreatitis in rats: Effects of a protease inhibitor, a beta - agonist, and antibiotics. Pancreas 1995; 11: 132-40.
  • 16. Buchler M, Fries H, Uhl W, Beeger HG: Clinical relevence of experimental acute pancreatitis. EurSurg Res 1992; 24 (Supl.l.): 85-88.
  • 17. Cao ZL , Okazaki Y, Naito K et al.:: Ulinastatin attenuates reperfusion injury in the isolated bloodperfused rabbit heart. Ann Thorac Surg 2000; 69; 1121-26.[Crossref]
  • 18. Hirano T, Manabe T: Human urinary tripsin inhibitor, urinastatin, prevents pancreatic injuris induced by pancreaticobiliary duct obstruction with cerulein stimulation and systemic hypotension in the rat. Arch Surg 1993; 128: 1322-29.[Crossref]
  • 19. Kakugawa Y, Takeda K, Kobari M: Effest of continous arterial infusion of protease inhibitor on experimental acute pancreatitis. Gastroenterol Jpn 1989; 24: 448-54.
  • 20. Kimura W, Furuhashi S, Zhang Q, Muto T: Effects of the Trypsin inhibitor Urinastatin on taurocholate-induced pancreatic cellular damage in rats. Pathophysiology 1996; 3: 21-28.[Crossref]
  • 21. Kobayashi H, Gotoh J, Terao T: Urinary trypsin inhibitor efficiently inhibits urokinase production in tumor necrosis factor-stimulated cells. Eur J CellBiol 1996; 71: 380-86.
  • 22. Kobayashi H, Shibata K, Fujie M, Terao T: Urinary tripsin inhibitor reduces the release of histamine from rat peritoneal mast cells. J LabClin Med 1998; 131: 375-85.
  • 23. Koizumi R, Kanai H, Maezawa A et al.: Terapeutic effects of ulinastatin on experimental crescentic glomerulonephritis in rats. Nephron 2000; 84: 347-53.[Crossref]
  • 24. Kruse P, Lasson A, Hage E: Proteases and protease inhibitors in cerulein- induced acute pancreatitis in rats. J Surg Res 1999; 85: 294-300.[PubMed][Crossref]
  • 25. McLatchie GR: Ostre zapalenie trzustki, w: Oksfordzki Podręcznik Chirurgii. PWN, Warszawa 1993; 428-37.
  • 26. Długosz JW: Patogeneza ostrego zapalenia trzustki - przegląd piśmiennictwa z ostatnich 2 lat. Magazyn Med - Lek Rodzinny 2000; 2: 3-44.
  • 27. Gabryelewicz A, Wereszczyńska-SiemiątkowskaU: Ostre zapalenie trzustki. W: Gastroenterologia i hepatologia kliniczna (S. Konturek red.), wyd. IV. PZWL, Warszawa 2001; 506-24.
  • 28. Komoriyama H, Tanaka I, Ikezawa H et al.: Continuous intraarterial infusion of protease inhibitors in acute pancreatitis. Drugs Today 2001; 37: 151-58.
  • 29. Takeda K, Sunamura M, Shibuya K et al.: Role of early continuous regional arterial infusion of protease inhibitor and antibiotic in nonsurgical treatment of acute necrotizing pancreatitis. Digestion 1999; 60: (suppl.l) 9-13.[Crossref]
  • 30. Takeda K, Kakugawa Y, Kobari M, Matsuno S: Continuous arterial infusion of inhibitor for severe acute pancreatitis. Gastroenterol Jpn 1989; 24: 340-48.[PubMed]
  • 31. Draus J, Maciejewski R, Mirosław C i wsp.: Elastaza neutrofilów jako marker diagnostyczny w doświadczalnym, ostrym zapaleniu trzustki. PolPrzegl Chir 1997; 69: 889-98.
  • 32. Maciejewski R, Madej B, Draus J et al.: Elastase activity and histological changes in lungs and in pancreas due to experimental pancreatitis. FoliaHistochem-Cytobiol 1999; 37: 103-04.
  • 33. M aciejewski R, Słomka M, Dąbrowski A i wsp.: Wpływ wzrostu ciśnienia w przewodzie trzustkowożółciowym na na aktywację lizosomów w trzustce. Pol Przegl Chir 1999; 71: 256-66.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_pjs-2013-0020
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.