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2013 | 1 | 39-55
Tytuł artykułu

Bis-pyridinium quadrupolar derivatives. High Stokes shift selective probes for bio-imaging

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
We describe the design, synthesis and characterization of five high Stokes shift quadrupolar heteroaryl compounds suitable as fluorescent probes in bio-imaging. In particular, we characterize the photophysical properties and the intracellular localization in Human Umbilical Vein Endothelial Cells (HUVEC) and Human Mesenchymal Stem Cells (HMSCs) for each dye. We show that, amongst all of the investigated derivatives, the 2,5-bis[1-(4-N-methylpyridinium)ethen-2-yl)]- N-methylpyrrole salt is the best candidates as selective mitochondrial tracker. Finally, we recorded the full emission spectrum of the most performing - exclusively mitochondrial selective - fluorescent probe directly from HUVEC stained cells. The emission spectrum collected from the stained mitochondria shows a remarkably more pronounced vibronic structure with respect to the emission of the free fluorophore in solution.
Wydawca

Rocznik
Tom
1
Strony
39-55
Opis fizyczny
Daty
otrzymano
2013-06-15
zaakceptowano
2013-07-17
online
2013-11-05
Twórcy
  • Department of Materials Science and INSTM,
    University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
  • CNR-ISTM, Institute of Molecular Science and Technology,
    via Golgi 19, I-20133 Milan, Italy
  • CNR-ISTM, Institute of Molecular Science and Technology,
    via Golgi 19, I-20133 Milan, Italy
  • Department of Materials Science and INSTM,
    University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
  • Department of Materials Science and INSTM,
    University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
Bibliografia
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  • [18] A. Baran, A. Plotniece, A. Sobolev, B. Vigante, M.Gosteva, V. Olkhovik, Chem. Heterocyc. Compd., 48,287-295 (2012)
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_oph-2013-0005
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