Czasopismo
Tytuł artykułu
Autorzy
Warianty tytułu
Języki publikacji
Abstrakty
We describe the design, synthesis and characterization of five high Stokes shift quadrupolar heteroaryl compounds suitable as fluorescent probes in bio-imaging. In particular, we characterize the photophysical properties and the intracellular localization in Human Umbilical Vein Endothelial Cells (HUVEC) and Human Mesenchymal Stem Cells (HMSCs) for each dye. We show that, amongst all of the investigated derivatives, the 2,5-bis[1-(4-N-methylpyridinium)ethen-2-yl)]- N-methylpyrrole salt is the best candidates as selective mitochondrial tracker. Finally, we recorded the full emission spectrum of the most performing - exclusively mitochondrial selective - fluorescent probe directly from HUVEC stained cells. The emission spectrum collected from the stained mitochondria shows a remarkably more pronounced vibronic structure with respect to the emission of the free fluorophore in solution.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
39-55
Opis fizyczny
Daty
otrzymano
2013-06-15
zaakceptowano
2013-07-17
online
2013-11-05
Twórcy
autor
-
Department of Materials Science and INSTM,
University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
autor
-
CNR-ISTM, Institute of Molecular Science and Technology,
via Golgi 19, I-20133 Milan, Italy
autor
-
CNR-ISTM, Institute of Molecular Science and Technology,
via Golgi 19, I-20133 Milan, Italy
autor
-
Department of Materials Science and INSTM,
University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
autor
-
Department of Materials Science and INSTM,
University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
autor
-
Department of Materials Science and INSTM,
University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy, giorgio.pagani@mater.unimib.it
autor
-
Department of Materials Science and INSTM,
University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy, luca.beverina@mater.unimib.it
Bibliografia
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- [15] V. Lebret et al., Chem. Mater., 20, 2174-2183 (2008)
- [16] Y. Suzuki, Y. Tenma, Y. Nishioka, K. Kamada, K. Ohta,J. Kawamata, J. Phys. Chem. C, 115, 20653-20661(2011)
- [17] X. Liu et al. Org. Biomol. Chem., 9, 3615 (2011)
- [18] A. Baran, A. Plotniece, A. Sobolev, B. Vigante, M.Gosteva, V. Olkhovik, Chem. Heterocyc. Compd., 48,287-295 (2012)
- [19] A. Abbotto, L. Beverina, G. A. Pagani, M. Collini, G.Chirico, L. D’Alfonso, G. Baldini, SPIE Proc., 5139,223-230 (2003)
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- [21] J. R. Lakowicz, Principles of fluorescence spectroscopy,3nd ed., (2008)
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_oph-2013-0005