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Fast signal analysis in Rotating-Polarization CARS microscopy

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Rotating Polarization Coherent Anti-Stokes Raman Spectroscopy (RP-CARS) is a novel approach to CARS microscopy that takes advantage of polarizationdependent selection rules in order to gain information about molecule orientation anisotropy and direction within the optical point spread function. However, in the original implementation of this technique, the lockin amplifier-based acquisition was quite time demanding. Here we present a new software-based approach that permits a great speed-up in the RP-CARS images acquisition process.
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  • Center for Nanotechnology
    Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San
    Silvestro 12, I-56127
  • NEST, Scuola Normale
    Superiore, Piazza San Silvestro 12, I-56127 Pisa, Italy
  • Center for Nanotechnology
    Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San
    Silvestro 12, I-56127
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