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Tytuł artykułu

Fabrication and Characterization of Three Dimensional Electrospun Cortical Bone Scaffolds

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Bone is a composite tissue composed of an organic matrix, inorganic mineral matrix and water. Structurally, bone is organized into two distinct types: trabecular (or cancellous) and cortical (or compact) bone. Cortical bone is highly organized, dense and composed of tightly packed units or osteons whereas trabecular bone is highly porous and usually found within the confines of cortical bone. Osteons, the subunits of cortical bone, consist of concentric layers of mineralized collagen fibers. While many scaffold fabrication techniques have sought to replicate the structure and organization of trabecular bone, very little research focuses on mimicking the organization of native cortical bone. In this study we fabricated three-dimensional electrospun cortical scaffolds by heat sintering individual osteon-like scaffolds. The scaffolds contained a system of channels running parallel to the length of the scaffolds, as found naturally in the haversian systems of bone tissue. The purpose of the studies discussed in this paper was to develop a mechanically enhanced biomimetic electrospun cortical scaffold. To that end we investigated the appropriate mineralization and cross-linking methods for these structures and to evaluate the mechanical properties of scaffolds with varying fiber angles. Cross-linking the gelatin in the scaffolds prior to the mineralization of the scaffolds proved to help prevent channels of the osteons from collapsing during fabrication. Premineralization, before larger scaffold formation and mineralization, increased mineral deposition between the electrospun layers of the scaffolds. A combination of cross-linking and premineralization significantly increased the compressive moduli of the individual scaffolds. Furthermore, scaffolds with fibers orientation ranging between 15° and 45° yielded the highest compressive moduli and yield strength.
Wydawca

Rocznik
Tom
2
Numer
1
Opis fizyczny
Daty
otrzymano
2014-02-17
zaakceptowano
2014-04-15
online
2014-11-12
Twórcy
autor
  • Virginia Tech-Wake Forest University,
    School of Biomedical Engineering and Sciences, Blacksburg, VA,
    USA 24061
  • Virginia Tech-Wake Forest University,
    School of Biomedical Engineering and Sciences, Blacksburg, VA,
    USA 24061
  • University of Virginia, Department of Biomedical
    Engineering, Charlottesville, VA
  • Virginia Tech-Wake Forest University,
    School of Biomedical Engineering and Sciences, Blacksburg, VA,
    USA 24061
  • Virginia Tech, Material Science and Engineering,
    Blacksburg, VA
  • Virginia Tech, Department of Chemical Engineering,
    Blacksburg, VA
  • Rutgers University,
    Department of Biomedical Engineering, Piscataway, NJ
  • Dominion University School of Medical Diagnostic & Translational
    Sciences, Norfolk, VA 23529, USA
Bibliografia
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  • [22] Heydarkhan-Hagvall, S.; Schenke-Layland, K.; Dhanasopon,A. P.; Rofail, F.; Smith, H.; Wu, B. M.; Shemin, R.; Beygui, R. E.;MacLellan, W. R., Three-dimensional electrospun ECM-basedhybrid scaffolds for cardiovascular tissue engineering.Biomaterials 2008, 29 (19), 2907-14[Crossref]
  • [23] Sisson, K.; Zhang, C.; Farach-Carson, M. C.; Chase, D. B.;Rabolt, J. F., Fiber diameters control osteoblastic cell migrationand differentiation in electrospun gelatin. J Biomed Mater ResA, 2010, 94 (4), 1312-24[WoS][PubMed]
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_nanome-2014-0002
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