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2014 | 2 | 1 |
Tytuł artykułu

Laminin- and basement membranepolycaprolactone blend nanofibers as a scaffold for regenerative medicine

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Mimicking one or more components of the basement membrane (BM) holds great promise for overcoming insufficiencies in tissue engineering therapies. We have electrospun laminin nanofibers (NFs) isolated from the murine Engelbreth-Holm Swarm (EHS) tumor and evaluated them as a scaffold for embryonic stem cell culture. Seeded human embryonic stem cells were found to better maintain their undifferentiated, colony environment when cultured on laminin NFs compared to laminin mats, with 75% remaining undifferentiated on NFs. Mouse embryonic stem cells cultured on 10% laminin-polycaprolactone (PCL) NFs maintained their colony formation for twice as long without passage compared to those on PCL or gelatin substrates. In addition, we have established a protocol for electrospinning reconstituted basement membrane aligned (RBM)-PCL NFs within 10° of angular deviation. Neuron-like PC12 cells show significantly greater attachment (p < 0.001) and percentage of neuriteextending cells in vitro on 10% RBM-PCL NFs when compared to 1% and 0% RBM-PCL NFs (p < 0.015 and p < 0.001, respectively). Together, these results implicate laminin- and RBM-PCL scaffolds as a promising biomimetic substrate for regenerative medicine applications.
Wydawca

Rocznik
Tom
2
Numer
1
Opis fizyczny
Daty
otrzymano
2014-01-01
zaakceptowano
2014-02-04
online
2014-09-01
Twórcy
  • University of Virginia Department of Biomedical Engineering,
    Charlottesville, VA 22903, USA
  • University of Virginia Department of Biomedical Engineering,
    Charlottesville, VA 22903, USA
autor
  • Georgia Institute of Technology and Emory University
    Department of Biomedical Engineering, Atlanta, Georgia 30332, USA
  • University of Virginia Department of Biomedical Engineering,
    Charlottesville, VA 22903, USA
  • University of Virginia Department of Biomedical Engineering,
    Charlottesville, VA 22903, USA
autor
  • Old Dominion University School of Medical Diagnostic
    & Translational Sciences, Norfolk, VA 23529, USA
  • Georgia Institute of Technology and Emory University
    Department of Biomedical Engineering, Atlanta, Georgia 30332, USA, edward.botchwey@bme.gatech.edu
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_nanome-2014-0001
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