Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 2 | 1 |
Tytuł artykułu

Selective determination of penicillin G from tap water and milk samples using surface molecularly imprinted polymers as solid-phase extraction sorbent

Treść / Zawartość
Warianty tytułu
Języki publikacji
In this study, a selective sample preparation method coupling surface molecularly imprinting polymers (SMIPs) with solid-phase extraction (SPE) was developed for the determination of penicillin G from tap water and milk samples. SMIPs for penicillin G were synthesized by using silica gel as supporting matrix, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EDMA) as the cross-linker, methanolacetonitrile (1:1,v/v) as the solvents and 2,2′-azo-bisisobutyronitrile (AIBN) as the initiator. Characterization and adsorption experiments revealed that SMIPs exhibited large adsorption capacity, high recognition ability and high rate of mass transfer. Application of SMIPs in SPE followed by HPLC was done to selectively determine penicillin G from tap water and milk samples. Under the optimal conditions, the proposed method demonstrated high linearity with the concentration of penicillin G ranging from 0.12 to 200 μg mL-1. The limits of detection (LOD) in tap water and milk were 0.05, 0.03μg mL-1, respectively. The limits of quantification (LOQ) in tap water and milk were 0.25, 0.12 μg mL-1, respectively. The recoveries of penicillin G at three spiked levels were ranged from 78.9 to 85.5 % with intra-day and inter-day relative standard deviation values below 4.8 %.

Opis fizyczny
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • [1] Boerlin P., Wissing A., Aarestrup F.M., Frey J., Nicolet J., Antimicrobialgrowth promotor ban and resistance to macrolides andvancomycin in Enterococci from pigs, J. Clin. Microbiol. 2001, 94,193-4195.
  • [2] Liu W., Zhang Z.J., Liu Z.Q., Determination of β-lactam antibioticsin milk using micro-flow chemiluminescence system with on-linesolid phase extraction, Anal. Chim. Acta. 2007, 592, 187-192.
  • [3] Palmer A.C., Angelino E., Kishony R., Chemical decay of anantibiotic inverts selection for resistance, Nat. Chem. Biol. 2010,6, 105-107.[WoS][Crossref]
  • [4] Junker T., Alexy R., Knacker T., Kümmerer K., Biodegradability of14C-Labeled antibiotics in a modified laboratory scale sewagetreatment plant at environmentally relevant concentrations,Environ. Sci. Technol. 2006, 40, 318-324.
  • [5] Kümmerer K., Resistance in the environment, J. Antimicrob.Chemother. 2004, 54, 311-320.
  • [6] Giger W., Alder A.C., Golet E., Kohler H.P.E., McArdell C.S., MolnarE., et al, Occurrence and fate of antibiotics as trace contaminantsin wastewaters, sewage sludges, and surface waters, Chimia.2003, 57, 485-491.
  • [7] Kolpin D., Furlong E.T., Meyer M.T., Thurman E.M., Zaugg S.D.,Barber L. B., et al, Pharmaceuticals, hormones, and otherorganic waste water contaminants in U.S. streams, 1999-2000:A national reconnaissance, Environ. Sci. Technol. 2002, 36,1202-1211.
  • [8] Golet E. M., Strehler A., Alder A.C., Giger W., Determination offluoroquinolone antibacterial agents in sewage sludge andsludge-treated soil using accelerated solvent extraction followedby solid-phase extraction, Anal. Chem. 2002, 74, 5455-5462.
  • [9] Denooz R., Charlier C., Simultaneous determination of fiveβ-lactam antibiotics (cefepim, ceftazidim, cefuroxim,meropenem and piperacillin) in human plasma byhigh-performance liquid chromatography with ultravioletdetection, J. Chromatogr. B. 2008, 86, 4161-167.
  • [10] Riediker S., Stadler R.H., Simultaneous Determination of fiveβ-lactam antibiotics in bovine milk using liquid chromatographycoupled with electrospray ionization tandem mass spectrometry,Anal. Chem. 2001, 73, 1614-1621.
  • [11] Bailón-Pérez M.I.,. García-Campaña A. M, Iruela M.D., Cruces-Blanco C., Gracia L. G., Multiresidue determination of penicillinsin environmental waters and chicken muscle samples by meansof capillary electrophoresis-tandem mass spectrometry, Electrophoresis.2009, 30, 1708-1717.
  • [12] Turiel E., Martín-Esteban A., Molecularly imprinted polymersfor sample preparation: A review, Anal. Chim. Acta. 2010, 668,87-99.
  • [13] Mahony J.O., Nolan K., Smyth M.R., Mizaikoff B., Molecularlyimprinted polymers-potential and challenges in analyticalchemistry, Anal. Chim. Acta. 2005, 534, 31-39.
  • [14] Pichon V., Chapuis-Hugon F., Role of molecularly imprintedpolymers for selective determination of environmentalpollutants-A review, Anal. Chim. Acta. 2008, 622, 48-61.
  • [15] Beltran A., Borrull F., Cormack P. A. G., MarcéR. M., Molecularlyimprintedpolymers: useful sorbents for selective extractions,Trac-Trends in Anal. Chem. 2010, 29, 1363-1375.
  • [16] Hiratsuka Y., Funaya N., Matsunaga H., Haginaka J., Preparationof magnetic molecularly imprinted polymers for bisphenol A andits analogues and their application to the assay of bisphenol A inriver water, J. Pharmaceut. Biomed. Anal. 2013, 75, 180-185.[WoS]
  • [17] Du W., Fu Q., Zhao G., Huang P., Jiao Y.Y., Wu H., Luo Z.M.,Chang C., Dummy-template molecularly imprinted solid phaseextraction for selective analysis of ractopamine in pork, FoodChem. 2013, 139, 24-30.
  • [18] Cederfur J., Pei Z.M.Y., Kempe M., Synthesis and screening of amolecularly Imprinted polymer library targeted for penicillin G, J.Comb. Chem. 2003, 5, 67-72.
  • [19] Beltran A., Fontanals N., Marce R.M., Cormack P.A.G., Borrull F.,Molecularly imprinted solid-phase extraction of cephalexin fromwater-based matrices, J. Sep. Sci. 2009, 32, 3319-3326.
  • [20] Yin Y.M., Chen Y.P., Wang X.F., Liu Y, Liu H.L., Xie M.X., Dummymolecularly imprinted polymers on silica particles for selectivesolid-phase extraction of tetrabromobisphenol A from watersamples. J. Chromatogr. A. 2012, 1220, 7-13.
  • [21] Zhao W.H., Sheng N., Zhu R., Wei F.D., Zheng C., Zhai M.J,, et al.Preparation of dummy template imprinted polymers at surfaceof silica microparticles for the selective extraction of tracebisphenol A from water samples. J. Hazard Mater. 2010, 179,223–229.[WoS]
  • [22] Gong X.Y., Cao X.J.. Preparation of molecularly imprintedpolymers for artemisinin based on the surfaces of silica gel, J.Biotechno. 2011 153, 8-14.
  • [23] Hua Z.D., Zhou S., Zhao M.P.. Fabrication of a surface imprintedhydrogel shell over silica microspheres using bovine serumalbumin as a model protein template, Biosens. Bioelectron.2009, 25,615-622.[WoS][Crossref]
  • [24] Yin J.F., Meng Z.H., Du M.J., Liu C., Song M.Y., Wang H.L.,Pseudo-template molecularly imprinted polymer for selectivescreening of trace β-lactam antibiotics in river and tap water, J.Chromatogr. A. 2010, 1217, 5420-5426.
  • [25] Zhang X.P., Chen L.G., Xu Y., Wang H., Zeng Q.L., Zhao Q., et al.Determination of β-lactam antibiotics in milk based on magneticmolecularly imprinted polymer extraction coupled with liquidchromatography-tandem mass spectrometry, J. Chromatogr. B.2010, 878, 3421-3426.
  • [26] Josefine C., Pei Y.X., Meng Z.H., Kempe M., Synthesis andScreening of a Molecularly Imprinted Polymer Library Targetedfor Penicillin G, J. Comb. Chem. 2003, 5, 67-72.
  • [27] ICH-International Conference on Harmonisation of TechnicalRequirements for Registration of Pharmaceuticals for HumanUse. Validation of analytical procedures: Text and methodologyQ2 (R1). In ICH Expert Working Group, ICH Harmonised TripartiteGuideline, Geneva, 2005, 1-13.
  • [28] He C.Y., Long Y.Y., Pan J.L., Li K., Liu F., Application of molecularlyimprinted polymers to solid-phase extraction of analytes fromreal samples, J. Biochem. Biophys. Methods. 2007, 70, 133-150.[WoS]
  • [29] Urraca J.L., Moreno-Bondi M.C., Hall A.J., Sellergren B.. Directextraction of penicillin G and derivatives from aqueous samplesusing a stoichiometrically imprinted polymer, Anal. Chem. 2007,79, 695-701.[WoS]
  • [30] Zhang J.K., Wang H., Liu W.S., Bai L.R., Ma N., Lu J.H.. Synthesisof molecularly imprinted polymer for sensitive penicillindetermination in milk, Anal. Lett. 2008, 41, 3411-3419.[WoS]
  • [31] Javanbakht M., Ali-Pishro K., Heidari-Nasab A., Akbari-aderganiB.. Extraction and purification of penicillin G from fermentationbroth by water-compatible molecularly imprinted polymers,Mater. Sci. Eng:C. 2012, 32, 2367-2373.[Crossref][WoS]
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.