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2012 | 1 | 55-64
Tytuł artykułu

Pressurized hot water extraction coupled to molecularly imprinted polymers for simultaneous extraction and clean-up of pesticides residues in edible and medicinal plants of the Okavango Delta, Botswana

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In this study, an in-cell extraction and clean-up approach, employing pressurized hot water extraction (PHWE) coupled to a molecularly imprinted polymer (MIP) is proposed. The selectivity of PHWE was improved through the use of a chlorophyll MIP (PHWE-MIP) for the determination of organochlorine pesticides residue levels in various edible and medicinal plants of the Okavango Delta, Botswana. The PHWE-MIP method achieved simultaneous extraction and clean-up. PHWE employed an optimal temperature of 260 °C, pressure of 90 bar and flow rate of 1 mL min-1 in 10 min for the extraction of the pesticides from plants while the MIP selectively overcame the interfering chlorophyll prior to analysis with gas chromatograph coupled to electron capture detector or mass spectrometer (GC-ECD/MS). The results obtained were compared to the QuEChERS Official Method 2007:01 for pesticides residue analysis. The proposed method seems to be nearly fully automated, environmental friendly, selective, simple and quick. Moreover, the recoveries of planar pesticides were improved (93-95%) with relative standard deviations (%RSD) of less than 10%.

Opis fizyczny
  • Department of Chemistry,
    Rhodes University, P O Box 94,
    Grahamstown, 6140, South Africa,
  • Department of Earth & Environmental
    Science (Chemistry Unit), Botswana
    International University of Science
    & Technology, P.O. Box 041, Bontleng,
    Gaborone, Botswana
  • University of Botswana - Okavango
    Research Institute, Private Bag 85,
    Maun, Botswana
  • Department of Chemistry,
    University of Botswana,
    Private Bag 00704, Gaborone,
  • Department of Chemistry,
    Rhodes University, P O Box 94,
    Grahamstown, 6140, South Africa
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