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Quantification of the titanium content in metallodrug-exposed tumor cells using HR-CS AAS

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
High-resolution continuum source atomic absorption spectroscopy (HR-CS AAS) is a valuable analytical technique for metal quantification because of its high sensitivity and selectivity for metal atoms as well as its improved background correction mode. However, the quantification of metals in biological materials, e.g. cell lysates, is still challenging because of matrix effects and other experimental complications. A method to quantify the titanium content of tumor cells exposed to titanium-based drugs was developed using HR-CS AAS. This method allows the quantification of titanium in cell suspensions in the low µg L-1 range with a detection limit of 48.8 µg L-1. The procedure was applied to the study of the cellular uptake of novel titanium metallodrugs (namely titanium (IV) salan complexes) and results showed a higher accumulation of these complexes in cancer cells compared to the titanium lead compound, titanocene dichloride. The improved cellular uptake of the studied complexes indicates a target located inside the cells and this could possibly lead to a higher antitumor effect of this novel class of metallodrugs. The antiproliferative potential of the complexes was confirmed in two different cancer cell lines, in which the titanium complexes showed good to moderate activity.
Wydawca

Czasopismo
Rocznik
Tom
1
Numer
1
Opis fizyczny
Daty
otrzymano
2013-09-27
zaakceptowano
2013-11-19
online
2014-03-10
Twórcy
autor
  • Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, 38106 Braunschweig, Germany
  • Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
  • Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
autor
  • Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, 38106 Braunschweig, Germany
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_medr-2014-0001
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