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2014 | 27 | 2 | 127-130
Tytuł artykułu

rapid detection for the inhibition of phosphoglucose isomerase from Escherichia coli by mercury(II) chloride based on TLC-autographic analysis – preliminary studies

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The quest for new techniques for screening inhibitors of phosphoglucose isomerase is crucially important owing to therapeutic control of chronic bacterial infections associated with the biosynthesis of bacterial biofilm. According to the new method, yellowish zones against the purple background could be visually observed where phosphoglucose isomerase activity was inhibited. The new protocol with NADPH/NBT/PMS staining for TLC-autographic method was able to detect PGI inhibition by pure reference substance as mercury(II) chloride.
Wydawca

Rocznik
Tom
27
Numer
2
Strony
127-130
Opis fizyczny
Daty
wydano
2014-06-01
otrzymano
2014-02-21
zaakceptowano
2014-08-11
online
2014-11-25
Twórcy
autor
  • Student of Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland
  • Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland
Bibliografia
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  • 9. Frasco M.F. et al.: Mechanism of cholinesterase inhibition by inorganic mercury. FEBS. J., 274, 1849, 2007.
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  • 22. Ramallo A., Zacchino S.A. and Furlan R.L.E.: A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers. Phytochem. Anal., 17, 15, 2006.[Crossref]
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_cipms-2014-0030
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