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Tytuł artykułu

Biosynthesis of ethyl butyrate with immobilized Candida rugosa lipase onto modified Eupergit®C

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Lipase from Candida rugosa was immobilized onto the modified Eupergit®C. The support was treated with ethylenediamine and subsequently activated with glutaraldehyde. Enzyme immobilization efficiency was 85%. The optimum pH was close to 6.5 for both the free and immobilized lipase. Immobilized lipase retained its maximum activity in a temperature range of 55 – 60°C. Subsequently, ethyl butyrate synthesis was investigated using immobilized enzyme by esterification of butyric acid with ethanol in solvent-free conditions (23% product yield) and using hexane as a solvent (65% product yield). The acid-alcohol molar ratio and different enzyme amounts were tested as efficient reaction parameters. The biocatalyst maintained 60% of its activity when reused in 8 successive batch reactions in organic solvent. Therefore, the immobilized lipase has demonstrated its potential in practical applications such as short-chain ester synthesis for the food industry.
EN
Wydawca

Czasopismo
Rocznik
Tom
1
Numer
1
Opis fizyczny
Daty
otrzymano
2014-06-22
zaakceptowano
2014-08-26
online
2014-10-03
Twórcy
  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy
autor
  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy
  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy
  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy, rebecca.pogni@unisi.it
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_boca-2014-0001
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