Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?
Background: The aim of the study was to designate changes in the expression of HSPA1A, HSPB1 and LDHb in elite rowers after completing a test “till exhaustion” on a rowing ergometer. Finally, we searched for the answer whether there are significant correlations between the expression of the genes and anaerobic threshold (AnT) or the maximal oxygen uptake (VO2max). Material/Methods: The research was conducted on the sample of 9 Polish lightweight male rowers (23.7 ±3.77 yrs, 72.7 ±1.76 kg, 183.6 ±4.58 cm). To determine AnT and VO2max, the subjects performed the test “till exhaustion” with an increasing load on a rowing ergometer. Directly before and after the test, blood samples were collected from the ulnar vein in order to isolate genetic material. RNA was extracted from white cells of venous blood by the chemical method. 2 μg RNA for the reverse transcription was used and the expression of HSPA1A, HSPB1 and LDHb was determined by Real time PCR reaction. To assess the intensity of expression, the ΔΔCt method was used. Results: The study showed an increased expression of HSPA1A and HSPB1 and a decreased one of LDHb. Moreover, post-training changes of the genes activity in white blood cells occurred immediately and could be determined directly after the termination of exertion. Conclusions: No significant correlations between the expression of the genes and anaerobic threshold (AnT), maximal oxygen uptake (VO2max) were stated
- 1. Morimoto RI. Regulation of the heat shock transcriptional response; cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Gen Develop. 1998;12:3788-3796.[Crossref]
- 2. Laszczynska J, Seweryn P. Indukcja bialka szoku termicznego HSP-70 w limfocytach ludzkich pod wplywem stresu cieplnego stymulujacego warunki nagrzanego kokpitu [Induction of heat shock protein HSP-70 in human lymphocytes under the influence of heat stress simulating the conditions of heated cockpit]. Polski Przeglad Med Lotn. 2007;4:435-443. Polish.
- 3. Radák Z, Naito H, Kaneko T, et al. Exercise training decreases DNA damage and increases DNA repair and resistance against oxidative stress of proteins in aged rat skeletal muscle. Pflugers Arch. 2002;445:273-278.
- 4. Kregel KC. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. Appl Physiol. 2002;92:2177-2186.
- 5. Arya R, Mallik M, Lakhotia SC. Heat shock genes-integrating cell survival and death. J Biosc. 2007;32:595-610.[PubMed]
- 6. Kazmierczuk A, Kilianska Z. Plejotropowa aktywnosc bialek szoku cieplnego [The pleiotropic activity of heat-shock proteins]. Postepy Hig Med Dosw. 2009;63:502-521. Polish.
- 7. Donnikov AE, Shkurnikov MU, Akimov EB, Tonevitsky AG. Relationship between the degree of cardiovascular adaptation and Th1/Th2 polarization of immune response. Bull Exp Biol Med. 2008;146:462-465.[WoS]
- 8. Fehrenbach E, Passek F, Nies AM, et al. HSP expression in human leukocytes is modulated by endurance exercise. Med Sci Sports Exerc. 2000;32:592-600.[Crossref][PubMed]
- 9. Sakharov DA, Maltseva DV, Riabenko EA, et al. Passing the anaerobic threshold is associated with substantial changes in the gene expression profile in white blood cells. Eur J Appl Physiol. 2012;112:963-972.[PubMed][Crossref]
- 10. Zeibig J, Karlic H, Lohninger A, Damsgaard R, Smekal G. Do blood cells mimic gene expression profile alterations known to occur in muscular adaptation to endurance training? Eur J Appl Physiol. 2005;95:96-104.[Crossref][PubMed]
- 11. Ecochard L, Lhenry F, Sempore B, Favier R. Skeletal muscle HSP72 level during endurance training: influence of peripheral arterial insufficiency. Pflugers Arch. 2000;40:918-924.[Crossref]
- 12. Maltseva DV, Ryabenko EA, Sizova SV, Yashin DV. Effect of exercise on the expression of HSPBP1, PGLYRP1, and HSPA1A genes in human leukocytes. Exp Bull Biol Med. 2012;53:867-869.
- 13. Ryan AJ, Gisolfi CV, Moseley PL. Synthesis of 70K stress protein by human leukocytes: effect of exercise in the heat. J Appl Physiol. 1991;70:466-471.[PubMed]
- 14. Liu Y, Lormes W, Baur C, et al. Human skeletal muscle Hsp70 response to physical training depends on exercise intensity. Int J Sports Med. 2000;21:351-355[Crossref]
- 15. Nielsen HG, Skjønsberg OH, Lyberg T. Effect of antioxidant supplementation on leucocyte expression of reactive oxygen species in athletes. San J Clin Lab Invest. 2008;68:526-533.[Crossref][WoS]
- 16. Mounier R, Pialoux V, Roels B, et al. Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes. Eur J Appl Physiol. 2009;105:515-524.[PubMed][Crossref][WoS]
- 17. Yoshioka M, Tanaka H, Shono N, Snyder EE, Shindo M, St-Amand J. Serial analysis of gene expression in the skeletal muscle of endurance athletes compared to sedentary men. Faseb J. 2003;17:1812-1819.[Crossref]
- 18. Hahn A, Bourdon P, Tanner R. Protocols for the physiological assessment of rowers. In: Gore C, ed. Physiological tests for elite athletes. Champaign, Ill.: Hum. Kinet. 2000; 311-326.
- 19. Ingham S, Whyte G, Neville JK. Determinants of 2000 m rowing ergometer performance in elite rowers. Eur J Appl Physiol. 2002;88:243-246.
- 20. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem. 1987;162:156-159.[Crossref]
- 21. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protocol. 2008;3:1101-1108.[Crossref]
- 22. Wang XY, Subject JR. High molecular weight stress protein: identification, cloning and utilization in cancer immunotherapy. Int J Hyperthermia. 2013;29:364-375 [Crossref][WoS]