Ultrasound-assisted Matrix Solid Phase Dispersion for the HPLC-DAD analysis of amphenicols in shrimps

• Autorzy: Victoria F. Samanidou , Evgenia A. Makrygianni
• Języki publikacji: EN

Abstrakty

EN

Ultrasound-assisted matrix solid phase dispersive extraction was applied for the selective isolation and clean-up of three amphenicol antibiotics, chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FFC) from shrimp. The target antibiotics were separated on a LiChroCART-LiChrospher® 100 RP-18 (5 μm, 250 × 4 mm) analytical column in less than 9 min, with isocratic elution using a mixture of 70% ammonium acetate (0.05 M) and 30% acetonitrile (v/v). Matrix Solid Phase Dispersion protocol was optimized in terms of extraction sorbent and elution solvent. Two polymer based (Oasis and Nexus) sorbents and one silica based (Lichrolut C18) were compared and different elution solvents such as methanol, acetone, acetonitrile and isopropanol were evaluated based on the achieved recovery rates as well as on the cleanup efficiency. The extraction procedure was performed with and without sonication to evaluate the impact of ultrasounds. TAP and FFC were monitored at 234 nm and CAP at 280 nm by a photodiode array detector. The method was validated according to the European Union Decision 2002/657/EC in terms of linearity, selectivity, stability, accuracy, precision and sensitivity. Detection capability values (CCb) were 64.6 μg/kg for TAP and 1046.8 μg/kg for FFC and 63.8 μg/ kg for CAP.

Słowa kluczowe

EN

HPLC; amphenicols; shrimps; ultrasoundassisted MSPD; 657/2002/EC

• Czasopismo: Sample Preparation
• Rocznik: 2014
• Tom: 2
• Numer: 1

Daty

otrzymano

2015-03-09

zaakceptowano

2015-05-06

online

2015-05-29

Twórcy

autor

Victoria F. Samanidou

• Laboratory of Analytical Chemistry, Department
  of Chemistry, Aristotle University of Thessaloniki, GR-54124,
  Thessaloniki, Greece

autor

Evgenia A. Makrygianni

• Laboratory of Analytical Chemistry, Department
  of Chemistry, Aristotle University of Thessaloniki, GR-54124,
  Thessaloniki, Greece

Bibliografia

• [1] Samanidou, V.F., Evaggelopoulou, E.N. Analytical strategiesto determine antibiotic residues in fish. J. Sep. Sci. 2007, 30,2549-2569.[WoS]
• [2] Commission Regulation (EU) No 37/2010 of 22 December 2009,L.15/1-72, on pharmacologically active substances and theirclassification regarding maximum residue limits in foodstuffsof animal origin
• [3] Commission Decision (EU) 181/2003, L71/17 of 13 March 2003,amending Decision 2002/657/EC as regards the setting ofminimum required performance limits(MRPLs) for certain residues in food of animal origin (notifiedunder document number C(2003) 764).
• [4] Douny, C., Widard, J., Paw, E., Guy, M-R., Scippo, M-L.Determination of Chloramphenicol in Honey, Shrimp, andPoultry Meat with Liquid Chromatography–Mass Spectrometry:Validation of the Method According to Commission Decision2002/657/EC. Food Anal. Meth. 2013, 6(5), 1458-1465.
• [5] Fernandez-Torres, R., Bello Lopez, M. A., Olías Consentino, M.,Callejón Mochón, M. Simultaneous determination of selectedveterinary antibiotics and their main metabolites in fish andmussel samples by High-Performance Liquid Chromatographywith Diode Array- Fluorescence (HPLC-DAD-FLD) Detection.Anal. Lett. 2011, 44, 2357–2372.
• [6] Gikas, E., Kormali, P., Tsipi, D. Tsarbopoulos A. Developmentof a rapid and sensitive SPE-LC-ESI MS/MS method for thedetermination of Chloramphenicol in Seafood. J. Agric. FoodChem. 2004, 52, 1025-1030.
• [7] Mottier, P., Prisod, V., Gremaud, E., Guy, P., Stadler, R.Determination of the antibiotic chloramphenicol in meat andseafood products by liquid chromatography–electrosprayionization tandem mass spectrometry. J. Chromatogr. A, 2003,994, 75–84.
• [8] Ramos, M., Muñoz, P., Aranda, A., Rodriquez, I., Diaz, R.,Blanca, J. Determination of chloramphenicol residues inshrimps by liquid chromatography–mass spectrometry. J.Chromatogr. B, 2003, 791, 31–38.
• [9] Thorser Rønning, H., Einersen, K., Normann Asp, T.Determination of chloramphenicol residues in meat, seafood,egg, honey, milk, plasma and urine with liquid chromatography–tandem mass spectrometry, and the validation of themethod based on 2002/657/EC. J. Chromatogr. A, 2006, 1118,226-233.
• [10] Dumont, V., Huet, A.-C., Traynor, I., Elliot, C., Delahaut,P. A surface plasmon resonance biosensor assay for thesimultaneous determination of thiamphenicol, florefenicol,florefenicol amine and chloramphenicol residues in shrimps.Anal. Chim.Acta, 2006, 567, 179–183.
• [11] Liu, W-L., Lee, R-J., Lee, M-R. (2010). Supercritical fluidextraction in situ derivatization for simultaneous determinationof chloramphenicol, florfenicol and thiamphenicol in shrimp.Food Chem., 121, 797-802.[WoS]
• [12] Tao, Y., Zhu, F., Chen, D., Wei, H., Pan, Y., Wang, X., Liu, Z.,Huang, L., Wang, Y., Yuan, Z. Evaluation of matrix solid-phasedispersion (MSPD) extraction for multi-fenicols determinationin shrimp and fish by liquid chromatography–electrosprayionisation tandem mass spectrometry. Food Chem., 2014, 150,500-506.
• [13] Turnipseed, S.B., Roybal, J.E., Phenning, A., Kijak, P.J. Use ofion-trap liquid chromatography–mass spectrometry to screenand confirm drug residues in aquacultured products. Anal.Chim.Acta, 2003, 483, 373–386.
• [14] Evaggelopoulou, E.N., Samanidou, V.F. Development andvalidation of an HPLC method for the determination of sixpenicillin and three amphenicol antibiotics in giltheadseabream (Sparus Aurata) tissue according to the EuropeanUnion Decision 2002/657/EC. Food Chem., 2013, 136,1322–1329.
• [15] Kubala-Drincic, H., Bazulic, D., Sapunar-Postrunznik, J.,Grubelic, M., Stuhne, G. Matrix Solid-Phase DispersionExtraction and Gas Chromatographic determination of Chloramphenicolin muscle tissue. Journal of Agricultural and Foodchem.., 2003, 51(4), 871-875.[Crossref]
• [16] Lu, Y., Zheng, T., He, X., Lin, X., Chen, L., Dai, Z. Rapiddetermination of chloramphenicol in soft-shelled turtle tissuesusing on-line MSPD-HPLC–MS/MS. Food Chem., 2012, 134,533-539.[WoS]
• [17] Karageorgou, E.G., Samanidou, V.F. Development andvalidation according to European Union Decision 2002/657/EC of an HPLC-DAD method for milk multi-residue analysis ofpenicillins and amphenicols based on dispersive extraction byQuEChERS in MSPD format. J. Sep. Sci., 2011, 34, 1893-1901.[WoS]
• [18] Roiha, I.S., Otterlei, E., Samuelsen O.B. Bioencapsulation ofFlorfenicol in Brine Shrimp, Artemia Franciscana, Nauplii. J.Bioanal. & Biomed., 2010, 2(3), 060-064.
• [19] Shakila, R.J., Saravanakumar, R., Princy Vyla, S.A., Jeyasekaran,G. An improved microbial assay for the detection of chloramphenicolresidues in shrimp tissues. Innov. Food Sci. & Emerg.Technol., 2007, 8, 515-518.[WoS]
• [20] Impens, S., Reybroeck, W., Vercammen, J., Courtheyn, D.,Ooghe, S., Wasch, K., Smedts, W., De Brabander, H. Screeningand confirmation of chloramphenicol in shrimp tissue usingELISA in combination with GC–MS2 and LC–MS2. Anal. Chim.Acta, 2003, 483, 153–163.
• [21] Bogialli, S., Di Corcia, A. Matrix solid-phase dispersion as avaluable tool for extracting contaminants from foodstuffs. J.Biochem. Biophys. Meth., 2007, 70, 163-179.[WoS]
• [22] Commission Decision (EU) 657/2002, L 221/8, of 12 August2002, implementing Council Directive 96/23/EC concerning theperformance of analytical methods and the interpretation ofresults, (notified under document number C(2002) 3044)

Identyfikatory

DOI

10.1515/sampre-2015-0003