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Tytuł artykułu

Integrated optical biosensor for rapid detection of bacteria

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In medical diagnostics, rapid detection of pathogenic bacteria from body fluids is one of the basic issues. Most state-of-the-art methods require optical labeling, increasing the complexity, duration and cost of the analysis. Therefore, there is a strong need for developing selective sensory devices based on label-free techniques, in order to increase the speed, and reduce the cost of detection. In a recent paper, we have shown that an integrated optical Mach-Zehnder interferometer, a highly sensitive all-optical device made of a cheap photopolymer, can be used as a powerful lab-on-a-chip tool for specific, labelfree detection of proteins. By proper modifications of this technique, our interferometric biosensor was combined with a microfluidic system allowing the rapid and specific detection of bacteria from solutions, having the surface of the sensor functionalized by bacterium-specific antibodies. The experiments proved that the biosensor was able to detect Escherichia coli bacteria at concentrations of 106 cfu/ml within a few minutes, that makes our device an appropriate tool for fast, label-free detection of bacteria from body fluids such as urine or sputum. On the other hand, possible applications of the device may not be restricted to medical microbiology, since bacterial identification is an important task in microbial forensics, criminal investigations, bio-terrorism threats and in environmental studies, as well.
Wydawca

Rocznik
Tom
2
Numer
1
Opis fizyczny
Daty
otrzymano
2015-06-01
zaakceptowano
2015-10-20
online
2015-12-31
Twórcy
autor
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Medical Microbiology,
    Semmelweis University, Budapest, Hungary
autor
  • Institute of Medical Microbiology,
    Semmelweis University, Budapest, Hungary
autor
  • Institute of Medical Microbiology,
    Semmelweis University, Budapest, Hungary
autor
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
Bibliografia
  • [1] D.N. Gilbert, B. Spellberg, J.G. Bartlett, An Unmet Medical Need:Rapid Molecular Diagnostics Tests for Respiratory Tract Infections,Clinical Infectious Diseases 52(S4), 2011, 384
  • [2] I. Abubakar, L. Irvine, C.F. Aldus, G.M. Wyatt, R. Fordham,S. Schelenz, L. Shepstone, A. Howe, M. Peck, P.R. Hunter,A systematic review of the clinical, public health and costeffectivenessof rapid diagnostic tests for the detection andidentification of bacterial intestinal pathogens in faeces andfood Health Technol. Assess. 11, 2007, 36
  • [3] A. Dér, S. Valkai, A. Mathesz, I. Andó, E.K. Wolff, P. Ormos,Protein-based all-optical sensor device, Sensors and ActuatorsB 151, 2010, 26
  • [4] A. Dér, L. Fábián, S. Valkai, J.J. Ramsden, P. Ormos, E.K. Wolff,Integrated optical switching based on the protein bacteriorhodopsin,Photochem. Photobiol. 83, 2007, 393[WoS][Crossref]
  • [5] K. Tiefenthaler, W. Lukosz, Sensitivity of grating couples as integratedoptical biosensors, J. Opt. Soc. Am. B 6, 1989, 209[Crossref]
  • [6] J. Vörös, J.J. Ramsden, G. Csúcs, I. Szendrő, S.M. De Paul, M.Textor, N.D. Spencer, Optical grating coupler biosensors, Biomaterials23, 2002, 3699[Crossref]
  • [7] P. Galajda, P. Ormos, Complex micromachines produced anddriven by light, Appl. Phys. Lett. 78, 2001, 249[Crossref]
  • [8] L. Bissonnette, M.G. Bergeron, Infectious DiseaseManagementthrough Point-of-Care Personalized Medicine Molecular DiagnosticTechnologies, J. Pers. Med. 2, 2012, 50[Crossref]
  • [9] J.J. Ramsden,S.-Y. Li, E. Heinzle, J.E. Prenosil, Optical methodfor measurement of number and shape of attached cells in realtime, Cytometry 19, 1995, 97[Crossref]
  • [10] R. Horváth, H.C. Pedersen, N. Skivesen, D. Selmeczi, N.B.Larsen, Optical waveguide sensor for on-line monitoring of bacteria,Optics Lett. 28 (2003) 1233-1235
  • [11] M. Miravitlles, A. Marín, E. Monsó, S. Vilŕ, C. de la Roza,R. Hervás, C. Esquinas, M. García, L. Millares, J. Morera, A. Torres,Colour of sputum is a marker for bacterial colonisationin chronic obstructive pulmonary disease, Respiratory Res. 11,2010, 58[WoS]
  • [12] M.L. Wilson, L. Gaido, Laboratory Diagnosis of Urinary Tract Infectionsin Adult Patients, Medical Microbiol. 38, 2004, 1150
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_optof-2015-0002
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