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2015 | 60 | 3 | 455-459
Tytuł artykułu

Impact of humic acids on EYL liposome membranes: ESR method

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this paper, the effects of model (commercial) and natural (extracted from peat) humic substances on the membrane of liposomes formed with egg yolk lecithin (EYL) are presented. In our research, mass concentrations of fulvic and humic acids were used, which in relation to lecithin varied from 0% to 13%. To study membrane fluidity, electron spin resonance (EPR) was used with two spin probes, penetrating various regions of the lipid bilayer. The effects of model and natural humic substances (humic acids – HAs and fulvic acids – FAs) on the lipid membrane in different regions were researched: the lipid-water interphase, and in the middle of the lipid bilayer. It was shown that FA and HA impact the fluidity of liposome membranes in different ways. Increased mass concentrations of HAs decreased membrane fluidity in both acids: extracted from peat and the model. However, increased mass concentration of FAs extracted from peat, decreased membrane fluidity in the surface region, at the same time stiffening the central part of the bilayer. Increasing the concentration of FAs extracted from peat had the opposite effect when compared to model FA. This effect may be related to the complexation of xenobiotics present in the soil environment and their impact on biological membranes.
Słowa kluczowe
Wydawca

Czasopismo
Rocznik
Tom
60
Numer
3
Strony
455-459
Opis fizyczny
Daty
wydano
2015-07-01
otrzymano
2014-10-01
zaakceptowano
2015-01-30
online
2015-08-06
Twórcy
  • Institute of Physics, Opole University, 48 Oleska Str., 45-052 Opole, Poland, Tel.: +48 77 452 7250, Fax: +48 77 452 7290
  • Department of Land Protection, Opole University, 22 Oleska Str., 45-052 Opole, Poland
  • Department of Land Protection, Opole University, 22 Oleska Str., 45-052 Opole, Poland
  • Institute of Physics, Opole University, 48 Oleska Str., 45-052 Opole, Poland, Tel.: +48 77 452 7250, Fax: +48 77 452 7290
autor
  • Institute of Physics, Opole University, 48 Oleska Str., 45-052 Opole, Poland, Tel.: +48 77 452 7250, Fax: +48 77 452 7290, dariusz.man@uni.opole.pl
Bibliografia
  • 1. Schnitzer, M., & Khan, S. U. (1978). Soil organic matter. Amsterdam: Elsevier.
  • 2. Pisarek, I. (2003). Characterization of humic substances formed in soil fertilized with sewage sludge and cattle manure. In Humic Substances in Ecosystems 5 (pp. 93–99). Duszniki Zdrój, Poland.
  • 3. Choudhry, G. G. (1984). Humic substances: structural, photophysical, photochemical and free radical aspects and interactions with environmental chemicals. New York: Gordon and Breach Science Publishers.
  • 4. Buffle, J. A. E. (1977). Les substances humiques et leurs interactions avec les ions mineraux. In Conference Proceedings de la Commission d’Hydrologie Appliquee de A.G.H.T.M. (pp. 3–10). l’Universite d’Orsay.
  • 5. Schulten, H. R., Plage, B., & Schnitzer, M. (1991). A chemical structure for humic substances. Naturwissenschaften, 78, 311–312.[Crossref]
  • 6. Jerzykiewicz, M., Czechowski, F., Jezierski, A., & Drozd, J. (1999). Influence of ammonia and nitrogen dioxide on free radicals in humic acids derived from composts, soil, peat and brown coal. An EPR study. Humic Subst. Environ., 1(3/4), 21–26.
  • 7. Pisarek, I., Głowacki, M., & Czernia, M. (2012). The impact of Pleurotus ostreatus on organic matter transformation processes. Water Sci. Technol., 66(12), 2660–2673. DOI: 10.2166/WST.2012.502.[Crossref]
  • 8. Paul, A., Stosser, R., Zehl, A., Zwirnmann, E., Vogt, R., & Steinberg, C. E. W. (2006). Nature and abundance of organic radicals in natural organic matter: Effect of pH and irradiation. Environ. Sci. Technol., 40, 5897–5903.[Crossref]
  • 9. Man, D., Słota, R., Mele, G., & Li, J. (2008). Fluidity of liposome membranes doped with metalloporphyrins: ESR study. Z. Naturforsch. C, 65, 440–444.
  • 10. Polewski, K., Sławińska, D., Sławiński, J., & Pawlak, A. (2005). The effect of UV and visible light radiation on natural humic acid. EPR spectral and kinetic studies. Geoderma, 126, 291–299.[Crossref]
  • 11. Boniewska-Bernacka, E., Man, D., Słota, R., & Broda, M. (2011). Effect of tin and lead chlorotriphenyl – analogues on selected living cells. J. Biochem. Mol. Toxicol., 25(4), 231–237.[Crossref][WoS]
  • 12. Mitrus, S., & Man, D. (2012). Effect of tin and lead chlorotriphenyl analogues on fruit fly Drosophila hydei and liposomes membrane. J. Biochem. Mol. Toxicol., 26(4), 162–167.[Crossref]
  • 13. Man, D., Podolak, M., & Engel, G. (2006). The influence of tin compounds on the dynamic properties of liposome membranes: A study using the ESR method. Cell. Mol. Biol. Lett., 11, 56–61.[Crossref]
  • 14. Man, D., Pisarek, I., Braczkowski, M., Pytel, B., & Olchawa, R. (2014). The impact of humic and fulvic acids on the dynamic properties of liposome membranes: the ESR method. J. Liposome Res., 24(2), 106–112.[Crossref][WoS]
  • 15. Soil Taxonomy. (1999). A basic system of soil classification for making and interpreting soil surveys. Soil Survey Staff. USDA-NRCS: US Gort. Printing Office, Washington, DC (Agric. Hand. 436).
  • 16. Man, D., Słota, R., Broda, M. A., Mele, G., & Li, J. (2011). Metalloporphyrin intercalation in liposome membranes: ESR study. J. Biol. Inorg. Chem., 16(1), 173–181.[Crossref]
  • 17. Shimshick, E. J., & McConnell, H. M. (1973). Lateral phase separation in phospholipid membranes. Biochemistry, 12, 2351–2360.[Crossref]
  • 18. Hemminga, M. A. (1973). Interpretation of ESR and saturation transfer ESR spectra of spin labeled lipids and membranes. Chem. Phys. Lipids, 32, 323–383.[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_nuka-2015-0081
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