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Tytuł artykułu

Selective adsorption of trypsin using molecularly imprinted polymers prepared with PEG-based hydrogels containing anionic functional monomers

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Molecularly imprinting (MI) hydrogels for selective adsorption of trypsin are reported. The trypsin imprinted hydrogels were prepared using a polyethylene glycol (PEG)-based dimethacrylate as a crosslinker and anionic functional monomers. The hydrogel prepared without any functional monomers showed significantly low ability to adsorb a variety of proteins. We optimized the concentration and the length of PEG units of the crosslinkers to achieve the complete removal of the template molecule and suitable selective adsorption. Additionally, the functional monomers chosen were anionic since the template, trypsin, is a basic protein. The adsorption tests for proteins, done on the prepared MI gels, indicated that the MI gel prepared with sodium allyl sulfonate (AS) as a functional monomer showed much higher selective adsorption for trypsin, even though a mixture of trypsin and cytochrome c was used as the protein solution. The selective adsorption was more effective in a NaCl solution in which the non-specific adsorption by a sulfonate is suppressed, similarly to our findings in a previous study. The MI gel prepared with acrylic acid also showed the selectivity, although the adsorption strength was lower than that of the MI gel containing AS. We believe that the present study constitutes the first approach for the selective adsorption of trypsin using PEG-based hydrogels.
Wydawca

Rocznik
Tom
2
Numer
1
Opis fizyczny
Daty
otrzymano
2015-07-31
zaakceptowano
2015-08-08
online
2016-02-11
Twórcy
autor
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan
autor
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_molim-2015-0004
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