PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
Czasopismo
2014 | 10 | 1 |
Tytuł artykułu

Effects of hypothermia on skeletal ischemia reperfusion injury in rats

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Objective: The aim of this study was to investigate the effect of hypothermia (H) on skeletal ischemia-reperfusion (IR) injury in rats by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nitric oxide (NO), and interleukin-1 beta (IL-1β) in muscle, and measureing immunohistochemical- inducible nitric oxide synthase (iNOS) staining of skeletal muscle. Materials and Methods: Eighteen Wistar Albino rats were divided randomly into three groups (sham, IR, hypothermia) (n=6). The sham group had all procedures without the IR period. The lower right extremity of rats in the IR and hypothermia groups was subjected to 2 hours of ischemia and 22 hours of reperfusion by applying a clamp on the common iliac artery and a rubber-band at the level of the lesser trochanter under general anesthesia. Rats in the hypothermia group underwent 4 hours of hypothermia during the first four hours of reperfusion in addition to a 2-hour ischemia and 22-hour reperfusion period. All rats were sacrificed at end of the IR period using a high dose of anesthesia. The tibialis anterior muscles were preserved. Immunohistochemical iNOS staining was performed, and MDA, SOD, GSH-Px, NO, and IL-1β were measured in the muscle. Results: The level of MDA, NO, and IL-1β in muscle was increased in the IR group compared with that in the sham group, but these parameters were decreased in the hypothermia group compared with the IR group. The activities of SOD and GSH-Px in muscle were decreased in the IR group; however, these parameters were increased in the hypothermia group. The score and intensity of iNOS staining of skeletal muscle was dens in IR group, mild in hypothermia group, and weak in sham group. Conclusion: The present study has shown that hypothermia reduced IR injury in the skeletal muscle by decreasing the levels of MDA, NO, and IL-1β, and increasing the activities of SOD and GSH-Px. In addition, hypothermia attenuated the score and intensity of iNOS staining.
Słowa kluczowe
EN
Wydawca

Czasopismo
Rocznik
Tom
10
Numer
1
Opis fizyczny
Daty
otrzymano
2014-04-14
zaakceptowano
2015-01-12
online
2015-02-27
Twórcy
  • Department of Orthopaedics and Traumatology, Malatya Military Hospital, Malatya, Turkey
  • Department of Emergency Medicine, Gulhane Military Medical Academy, Ankara, Turkey
  • Department of Orthopaedics and Traumatology, Malatya Military Hospital, Malatya, Turkey
  • Department of Orthopaedics and Traumatology, Haydarpaşa Military Hospital, İstanbul, Turkey
autor
  • Department of Orthopaedics and Traumatology, Gulhane Military Medical Academy, Ankara, Turkey
  • Department of General Surgery, Mevki Military Hospital, Ankara, Turkey
  • Department of Emergency Medicine, Gulhane Military Medical Academy, Ankara, Turkey
  • Department of Orthopaedics and Traumatology, Bursa Military Hospital, Bursa, Turkey
  • Department of Orthopaedics and Traumatology, Gulhane Military Medical Academy, Ankara, Turkey
autor
  • Department of Orthopaedics and Traumatology, Erzurum Military Hospital, Erzurum, Turkey
autor
  • Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey
  • Department of Orthopaedics and Traumatology, Gulhane Military Medical Academy, Ankara, Turkey
Bibliografia
  • [1] Koca K, Yurttas Y, Bilgic S, Cayci T, Topal T, Durusu M, KaldirimU, Akgul EO, Ozkan H, Yanmis I, Oguz E, Tunay S, Korkmaz A,Basbozkurt M. Effect of Preconditioned hyperbaric oxygenandozone on ischemia-reperfusion induced tourniquet in skeletalbone of rats. J SurgRes. 2010 Nov;164(1):e83-89
  • [2] M.O. Taha, M.J. Simões, E.C. Noguerol, F.P. Mendonça, H.M.A.Pascoalick, R.A.M. Alves,M.E.M.Vivian, F.P. Morales, A.C.A.Campos, K.G. Magalhães, P.S. Venerando, I.L.S. Tersariol,H.P.Monteiro, I. Oliveira, Jr, A. Jurkiewicz, and A. Caricati-Neto.Effects of allopurinol on ischemia and reperfusion in rabbitlivers. Transplant Proc. 2009 Apr;41(3): 820-823[Crossref]
  • [3] Scarfiotti C, Fabris F, Cestaro B, Giuliani A: Freeradicals,atherosclerosis, ageing, and related dysmetabolic pathologies:pathological and clinical aspects. Eur J Cancer Prev Suppl 1:31-36, 1997
  • [4] Tunç T, Öter S, Güven A, Topal T, Kul M, Korkmaz A, et al.Protectiveeffect of sulfhydryl-containing antioxidants againstischemia/reperfusioninjury of prepubertal rat intestine. JGastroenterol Hepatol 2009;24:681-687[Crossref]
  • [5] Beris AE, Soucacos PN, Seaber AV, Urbaniak JR. Effects ofcold ischemia on reflow patterns in the rat cremaster musclemicrocirculation. Int Angiol 1995 Sep;14(3):248-252
  • [6] Wright JG, Araki CT, Belkin M, Hobson RW. Postischemichypothermia diminishes skeletal muscle reperfusion edema. JSurgRes 1989 Now;47(5):389-396
  • [7] Wright JG, Belkin M, Hobson RW. Hypothermia and controlledreperfusion: Twonon-pharmacological methods which diminishischemia-reperfusion injury in skeletal muscle. MicrocircEndothelium Lymphatics. 1989 Jun-Oct; 5(3-5):315-334
  • [8] Mowlavi A, Neumeister MW, Wilhelmi BJ, Song YH, Suchy H,Russell RC. Local Hypotermia during early reperfusion protectsskeletal muscle from ischemia-reperfusion injury. Plast RecontrSurg.2003 Jan 111(1):242-250
  • [9] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Proteinmeasurement with the folin-phenol reagent. J BiolChem 1951:193: 265-275
  • [10] Okhawa H, Ohshi N, Yagi K. Assay or lipid peroxides in animaltissues by thiobarbituric acid reaction. Anal Biochem 1979: 95:351-358
  • [11] Sun Y, Oberley LW, Ying L. A simple method for clinical assay ofsuperoxidedismutase.Clin Chem 1988: 34: 497-500
  • [12] Paglia DE, Valentine WN. Studies on the quantitative andqualitative characterization of erythrocyte glutathioneperoxidase. J Lab Clin Med 1967: 70: 158-169
  • [13] Koca K, Yurttas Y, Yıldız C, Çaycı T, Uysal B, Korkmaz A.Effect of hyperbaric oxygen and ozone preconditioning onoxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthop Traumatol Turc.2010;44(6):476-483[WoS][Crossref]
  • [14] Canevari L, Console A, Tendi EA, Clarck JB, Bates TE. Effectof postischemia hypothermia on the mitochondrial damageinduced by ischemia and reperfusion in the gerbil. Brain Res.1999 Jan 30;817(1-2):241-245
  • [15] Weinrauch V, Safar P, Tisherman S, Kuboyama K, Radovsky A.Beneficial effect of mild hypothermia and detrimental effectof deep hypothermia after cardiac arrest in dogs. Stroke1992Oct;23(10)1454-1462[Crossref]
  • [16] Armstead WM, Mirro R, Busija DW, Leffler CW. Postischemicgeneration of superoxide anion by newborn pig brain. Am JPhysiol. 1988 Aug;255(2 Pt 2):H401-3
  • [17] Sapega AA, Heppenstall RB, Sokolow DP, Graham TJ, MarisJM, Ghosh AK, Chance B, Osterman Lai. The bioenergetics ofpreservation in the limbs before reimplantation. J Bone JointSurg Am. 1988 Dec;70(10):1500-1513[Crossref]
  • [18] Zhang JX, Wolf MB. Effect of cold on ischemia-reperfusioninduced microvascular permeability increase in cat skeletalmuscle. Cryobiology. 1994 feb:31(1);94-100[Crossref]
  • [19] Laptook AR, Corbett RJ, Sterett R, Burns DK, Garcia D, TollefsbolG. Modest Hypotermia provides partial neuroprotection whenused for immediate resuscitation after brain ischemia. PediatrRes. 1997 Jul:42(1);17-23[Crossref]
  • [20] Lei B, Tan X, Cai H, Xu Q, Guo Q. Effect of moderatehypothermia on lipid peroxidation in canine brain tissue aftercardiac arrest and resuscitation. Stroke. 1994;25:147-152[Crossref]
  • [21] Dos Santos EB, Koff WJ, Grezzana Filho Tde J, De Rossi SD, TreisL, Bona SR, Pegas KL, Katz P, Meyer FS, Marroni NA, Corso CO.Oxidative stress evaluation of ischemia and reperfusion inkidneys under various degrees of hypothermia in rats. Acta CirBra. 2013 Aug; 28 (8):568-573[Crossref]
  • [22] Grezzana Filho Tde J, Mendonça TB, Gabiatti G, RodriquesG, Marroni NA, Treis L, De Rossi SD, Corso CO. Topic liverhypothermia and ischemic preconditioning: a new modelof ischemia and reperfusion in rats. Acta Cir Bras. 2011Jun;26(3):194-201[Crossref]
  • [23] Beckman JS, Koppenol WH. Nitric oxide, superoxide, andperoxynitrite: the good, the bad, and ugly. Am J Physiol. 1996Nov: 271 (5 Pt 1):C1424-1437
  • [24] Guven, A, Gundogdu G, Uysal B, Cermik H, Kul M, DemirbagS, Ozturk H, Oter S., Hyperbaric oxygen therapy reduces theseverity of necrotizing enterocolitis in a neonatal rat model. JPediatr Surg. 2009 Mar; 44(3): 534-540[Crossref]
  • [25] Gibbons H, Sato TA, Draqunow M. Hypothermiasuppresses inducible nitric oxide synthase and stimulatescyclooxygenase-2 in lipopolysaccharide stimulated BV-2 cells.Brain Res Mol Brain Res. 2003 Jan 31;110(1):63-75
  • [26] Avci G, Kadioglu H, Sehirli AO, Bozkurt S, Guclu O, Arslan E,Muratli SK. Curcumin protects against ischemia/reperfusioninjury in rat skeletal muscle. J Surg Res. 2012 Jan;172(1):e39-46.doi: 10.1016/j.jss.2011.08.021. Epub 2011 Sep 13[Crossref]
  • [27] Zhang H, Zhou M, Zhang J, Mei Y, Sun S, Tong E. Therapeuticeffect of post-ischemic hypothermia duration on cerebralischemic injury. Neurol Res. 2008 May;30(4):332-6. doi:10.1179/174313208X300279[Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_med-2015-0031
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.