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2014 | 10 | 1 |
Tytuł artykułu

Integration of human papillomavirus type 16 in cervical cancer cells

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cervical cancer remains an important cause of women morbidity and mortality. The progression of cervical pathology correlates with the HPV integration into the host genome. However, the data on the viral integration status in cervical dysplasias are controversial. The aim of the current study was to evaluate the status of HPV integration in two types of cervical pathology – invasive and non invasive cervical cancer (e.g. carcinoma in situ). 156 women were included in the study: 66 women were diagnosed with invasive cervical cancer (CC) and 90 with non invasive cervical cancer (carcinoma in situ, CIS). 74.2% [95% PI: 63.64÷84.76] of specimens collected from women with diagnosed CC and 85.6% [95% PI: 85.53÷92.85] of CIS specimens were positive for HPV. The most prevalent HPV genotype in both groups was HPV16. To evaluate HPV integration, three selected HPV16 E2 gene fragments were analyzed by PCR. In the majority of CC and CIS specimens the amplification of all three HPV16 E2 gene fragments was observed. The episomal HPV16 form was detected in the majority of CC and CIS specimens. The deletion of all three HPV16 E2 gene fragments was detected in 9.4% of CC specimens and 2.2% of CIS specimens. Finally, integration status could not be used as diagnostical additional test to distinguish between invasive and non invasive cervical cancer.
Wydawca

Czasopismo
Rocznik
Tom
10
Numer
1
Opis fizyczny
Daty
otrzymano
2013-07-26
zaakceptowano
2014-06-10
online
2014-09-25
Twórcy
  • Institute of Oncology, Vilnius University, Santariskiu str. 1, LT-08660 Vilnius, Lithuania
  • Institute of Oncology, Vilnius University, Santariskiu str. 1, LT-08660 Vilnius, Lithuania
  • Vilnius University, Universiteto str. 3, LT-01513 Vilnius, Lithuania
  • Vilnius University, Universiteto str. 3, LT-01513 Vilnius, Lithuania
  • Vilnius University, Universiteto str. 3, LT-01513 Vilnius, Lithuania
  • Institute of Biotechnology of Vilnius University, V.A.Graiciuno 8, LT-02241 Vilnius, Lithuania
  • Institute of Biotechnology of Vilnius University, V.A.Graiciuno 8, LT-02241 Vilnius, Lithuania
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_med-2015-0001
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