Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 1 | 1 |
Tytuł artykułu

Survival of Bifidobacteria and their usefulness in Faecal Source Tracking

Treść / Zawartość
Warianty tytułu
Języki publikacji
Bifidobacteria have long since been recommended as indicators of human and animal pollution. Concentration ratio (tracking ratio) of the sorbitol-utilising bifidobacteria (SUB) and the total bifidobacteria (TB) can be used to distinguish between animal and human sources of faecal water contamination. The cut-off value needs to be calibrated in a given geographical area. Seven sites with permanent faecal contamination were selected in South Africa. Concentrations of SUB ranged from 10-50000 cells/100 mL, while TB ranged from 0-8000 cells/100 mL. The tracking ratio ranged from 0.10 to 6.25, but no clear cut-off value could be established. The YN-17 agar was replaced for TB with the modified Beerens medium with pH = 5.70, to suppress the growth of faecal streptococci. Tracking ratios observed are most likely the results of different survival rates of SUB and TB. Bifidobacteria die-off due to nutrients was not found to be significant using design of experiment. Thus a lack of continuous input or oxygen levels in water may be major factors. This would limit the ratios used as a faecal source tracking method.
Słowa kluczowe

Opis fizyczny
  • Environmental Health and Biotechnology
    Research Group, Division of Pharmaceutical Chemistry, Faculty of
    Pharmacy, Rhodes University, South Africa
  • Division of Pharmaceutics, Faculty of Pharmacy,
    Rhodes University, South Africa
  • Amatole Waterboard, East London, South Africa
  • Environmental Health and Biotechnology
    Research Group, Division of Pharmaceutical Chemistry, Faculty of
    Pharmacy, Rhodes University, South Africa
  • AHMED, W., STEWART, J., GARDNER, T., POWELL, D., BROOKS, P., SULLIVAN, D. & TINDALE, N. 2007. Sourcing faecal pollution: A Combination of Library-Dependent and Library-Independent Methods to Identify Human Faecal Pollution in Non-Sewered Catchments. Water Research, 41, 3771-3779.[WoS][Crossref]
  • BALFOUR, F., BADENHORST, H. & TROLLIP, D. 2011. A Gap Analysis of Water Testing Laboratories in South Africa. Pretoria, South Africa: Water Research Commission.
  • BALLESTÉ, E. & BLANCH, A. R. 2011. Bifidobacterial Diversity and the Development of New Microbial Source Tracking Indicators. Applied and Environmental Microbiology, 77, 3518-3525.[WoS]
  • BALLONGUE, J. 2004. Bifidobacteria and Probiotic Action. In: SALMINEN, S., WRIGHT, A. V. & OUWEHAND, A. (eds.) Lactic Acid Bacteria: Microbiological and Functional Aspects, Third Edition, Revised and Expanded. Third Edition, Revised and Expanded ed. USA: Marcel Dekker, Inc.
  • BEERENS, H. 1990. An elective and selective isolation medium for Bifidobacterium spp. Letters in applied microbiology, 11, 155-157.[Crossref]
  • BLANCH, A. R., BELANCHE-MUNOZ, L., BONJOCH, X., EBDON, J., GANTZER, C., LUCENA, F., OTTOSON, J., KOURTIS, C., IVERSEN, A., KUHN, I., MOCE, L., MUNIESA, M., SCHWARTZBROD, J., SKRABER, S., PAPAGEORGIOU, G. T., TAYLOR, H., WALLIS, J. & JOFRE, J. 2006. Integrated Analysis of Established and Novel Microbial and Chemical Methods for Microbial Source Tracking. Applied and Environmental Microbiology, 72, 5915-5926.[Crossref]
  • BOLDUC, M.-P., RAYMOND, Y., FUSTIER, P., CHAMPAGNE, C. P. & VUILLEMARD, J.-C. 2006. Sensitivity of bifidobacteria to oxygen and redox potential in non-fermented pasteurized milk. International Dairy Journal, 16, 1038-1048.[Crossref]
  • BONJOCH, X., BALLESTÉ, E. & BLANCH, A. R. 2005. Enumeration of Bifidobacterial Populations with Selective Media to Determine the Source of Waterborne Fecal Pollution. Water Research, 39, 1621-1627.
  • BONJOCH, X., LUCENA, F. & BLANCH, A. R. 2009. The persistence of bifidobacteria populations in a river measured by molecular and culture techniques. Journal of Applied Microbiology, 107, 1178-1185.[WoS]
  • BOX, G. E. P. & WILLIAM, J. S. H. 2005. Statistics for Experimenters : Design, Innovation, and Discovery New York, John Wiley and Sons.
  • BURTON, G. A., GUNNISON, D. & LANZA, G. R. 1987. Survival of pathogenic bacteria in various freshwater sediments. Applied and Environmental Microbiology, 53, 633-638.[WoS]
  • CARRILLO, M., ESTRADA, E. & HAZEN, T. C. 1985. Survival and enumeration of the fecal indicators Bifidobacterium adolescentis and Escherichia coli in a tropical rain forest watershed. Applied and Environmental Microbiology, 50, 468-476.
  • CIMENTI, M., HUBBERSTEY, A., BEWTRA, J. K. & BISWAS, N. 2007. Alternative Methods in Tracking Sources of Microbial Contamination in Waters. Water SA, 33, 183-194.
  • GEERAERD, A. 2012. GInaFiT: Geeraerd and Van Impe Inactivation model Fitting Tool. 1.6 ed. Belgium: Katholieke Universiteit Leuven (Belgium).
  • GYLLENBERG, H., NIEMELA, S. & SORMUNEN, T. 1960. Survival of bifid bacteria in water as compared with that of coliform bacteria and enterococci. Applied Microbiology, 8, 20-22.
  • HAGEDORN, C., BLANCH, A. R. & HARWOOD, V. J. (eds.) 2011. Microbial Source Tracking: Methods, Applications, and Case Studies: Springer.
  • HANSEN, L. T., ALLAN-WOJTAS, P. M., JIN, Y. L. & PAULSON, A. T. 2002. Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions. Food Microbiology, 19, 35-45.
  • JAGALS, P. & GRABOW, W. 1996. An evaluation of sorbitol-fermenting bifidobacteria as specific indicators of human faecal pollution of environmental water. WATER SA-PRETORIA-, 22, 235-238.
  • JAYAMANNE, V. S. & ADAMS, M. R. 2006. Determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts. Letters in applied microbiology, 42, 189-194.[Crossref]
  • JAYAMANNE, V. S. & ADAMS, M. R. 2009. Modelling the effects of pH, storage temperature and redox potential (Eh) on the survival of bifidobacteria in fermented milk. International Journal of Food Science & Technology, 44, 1131-1138.
  • LALIBERTE, P. & GRIMES, D. J. 1982. Survival of Escherichia coli in lake bottom sediment. Applied and Environmental Microbiology, 43, 623-628.
  • LEWIN, S., NORMAN, R., NANNAN, N., THOMAS, E., BRADSHAW, D. & COLLABORATION, S. A. C. R. A. 2007. Estimating the burden of disease attributable to unsafe water and lack of sanitation and hygiene in South Africa in 2000. South African Medical Journal, 97, 755-762.
  • LUYT, C. D., TANDLICH, R., MULLER, W. J. & WILHELMI, B. S. 2012. Review: Microbial Monitoring of Surface Water in South Africa: An Overview. International Journal of Environmental Research and Public Health 9, 2669-2693.[WoS]
  • MARA, D. D. & ORAGUI, J. I. 1983. Sorbitol-fermenting bifidobacteria as specific indicators of human faecal pollution. Journal of applied microbiology, 55, 349-357.
  • MATSUMOTO, M., OHISHI, H. & BENNO, Y. 2004. H+-ATPase activity in Bifidobacterium with special reference to acid tolerance. International journal of food microbiology, 93, 109-113.
  • MEAYS, C. L., BROERSMA, K., NORDIN, R. & MAZUMDER, A. 2004. Source tracking fecal bacteria in water: a critical review of current methods. Journal of environmental management, 73, 71-79.[Crossref]
  • MURRAY, K., DU PREEZ, M., KUHN, A. & VAN NIEKER, H. 2004. A Pilot Study to Demonstrate Implementation of the National Microbial Monitoring Programme. Pretoria, South Africa: Report to the Water Research Commission.
  • MUSHI, D., BYAMUKAMA, D., KIVAISI, A. K., MACH, R. L. & FARNLEITNER, A. H. 2010. Sorbitol-Fermenting Bifidobacteria are Indicators of Very Recent Human Faecal Pollution in Streams and Groundwater Habitats in Urban Tropical Lowlands. Journal of Water and Health, 8, 466-478.[WoS][Crossref]
  • NEBRA, Y., JOFRE, J. & BLANCH, A. R. 2002. The effect of reducing agents on the recovery of injured Bifidobacterium cells. Journal of microbiological methods, 49, 247-254.
  • OTTOSON, J. R. 2009. Bifidobacterial survival in surface water and implications for microbial source tracking. Canadian journal of microbiology, 55, 642-647.[WoS]
  • PLYMOUTH MARINE LABORATORY 2009. Primer 6 & Permanova +. 6.1.13 & 1.03 ed. United Kingdom: Primer - E.
  • PMG, P. M. G. 2009. Water Cuts & Cholera Situation in City of Cape Town: City, Departmental & Civil Community Responses. . Available:
  • PONTES, K. V., WOLF MACIEL, M. R., MACIEL, R. & EMBIRUÇU, M. 2011. Process analysis and optimization mapping through design of experiments and its application to a polymerization process. Brazilian Journal of Chemical Engineering, 28, 137-150.[Crossref][WoS]
  • RELIASOFT CORPARATION 2011. DOE ++. 1.0.7 ed.: ReliaSoft Corparation,.
  • RESNICK, I. G. & LEVIN, M. A. 1981. Assessment of bifidobacteria as indicators of human fecal pollution. Appl. Environ. Microbiol., 42, 433-438.
  • ROLFE, R. D., HENTGES, D. J., BARRETT, J. T. & CAMPBELL, B. J. 1977. Oxygen tolerance of human intestinal anaerobes. The American Journal of Clinical Nutrition, 30, 1762-9.
  • SAARELA, M., ALAKOMI, H. L., MÄTTÖ, J., AHONEN, A. M., PUHAKKA, A. & TYNKKYNEN, S. 2010. Improving the storage stability of Bifidobacterium breve in low pH fruit juice. International journal of food microbiology, In Press, Corrected Proof.
  • SALMINEN, S., VON WRIGHT, A. & OUWEHAND, A. 2004. Lactic Acid Bacteria: Microbiological and Functional Aspects, Marcel Dekker, Inc.
  • SCOTT, T. M., ROSE, J. B., JENKINS, T. M., FARRAH, S. R. & LUKASIK, J. 2002. Microbial Source Tracking: Current Methodology and Future Directions. Appl. Environ. Microbiol., 68, 5796-5803.
  • SHAH, N. P. & LANKAPUTHRA, W. E. V. 1997. Improving viability of Lactobacillus acidophilus and Bifidobacterium spp. in yogurt. International Dairy Journal, 7, 349-356.[Crossref]
  • SHAH, N. P., LANKAPUTHRA, W. E. V., BRITZ, M. L. & KYLE, W. S. A. 1995. Survival of Lactobacillus acidophilus and Bifidobacterium bifidum in commercial yoghurt during refrigerated storage. International Dairy Journal, 5, 515-521.[Crossref]
  • SHERER, B. M., MINER, J. R., MOORE, J. A. & BUCKHOUSE, J. C. 1992. Indicator Bacterial Survival in Stream Sediments. J. Environ. Qual., 21, 591-595.[Crossref]
  • SINTON, L. W., FINLAY, R. K. & HANNAH, D. J. 1998. Distinguishing Human from Animal Faecal Contamination in Water: A Review. New Zealand Journal of Marine and Freshwater Research, 32, 323 - 348.[Crossref]
  • TALWALKAR, A. & KAILASAPATHY, K. 2004. A Review of Oxygen Toxicity in Probiotic Yogurts: Influence on the Survival of Probiotic Bacteria and Protective Techniques. Comprehensive Reviews in Food Science and Food Safety, 3, 117-124.
  • TANDLICH, R., LUYT, C. & MULLER, W. 2012. Faecal contamination source identification using a combination of chemical and microbial biomarkers. Pretoria: Division of Pharmaceutical Chemistry, Faculty of Pharmacy, Rhodes University; Unilever Centre for Environmental Water Quality Institute for Water Research Rhodes University.
  • WANG, Y.-C., YU, R.-C. & CHOU, C.-C. 2002. Growth and survival of bifidobacteria and lactic acid bacteria during the fermentation and storage of cultured soymilk drinks. Food Microbiology, 19, 501-508.[Crossref]
  • WILSON, M. 2005. Microbial Inhabitants of Humans: Their ecology and role in health and disease, Cambridge, United Kingdom, Cambridge University Press.
  • ZUMA, B. M. 2010. Microbial Ecology of the Buffalo River in Response to Water Quality Changes. Msc, Rhodes University.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.