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2015 | 28 | 1 | 17-20
Tytuł artykułu

The alteration in peripheral neutrophils of patients with chronic kidney disease

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Recent findings have demonstrated the impaired functions of neutrophils of patients with chronic renal failure. The purpose of our research was to study oxidative modified proteins, as well as the histone spectrum in neutrophils drawn from patients with chronic kidney disease, and to estimate the ability of neutrophils to form spontaneous neutrophil extracellular traps. In this work, we have assumed that metabolic alteration in neutrophils may develop at early stages of chronic kidney disease. Materials and methods: Neutrophils obtained from patients with various stages of chronic kidney disease and degrees of chronic renal failure were used. As control, blood samples obtained from 32 healthy individuals was employed. In the examined neutrophils, advanced oxidation protein products, protein reactive carbonyl derivatives, as well as nucleosomal histones were detected. The ability of neutrophils to form spontaneous neutrophil extracellular traps was estimated. Our results have demonstrated an increase of nucleosomal histones in neutrophils of all patients with chronic kidney disease. Moreover, our work fixes the rate of growth of intracellular advanced oxidation protein products and the decreasing of protein reactive carbonyl derivatives in neutrophils from patients with chronic kidney disease. Furthermore, we demonstrate the presence of the neutrophils with altered oxidative status and the decomposition of the histone spectrum in the circulation of patients with chronic kidney disease.
Wydawca

Rocznik
Tom
28
Numer
1
Strony
17-20
Opis fizyczny
Daty
wydano
2015-03-01
otrzymano
2015-01-07
zaakceptowano
2015-02-16
online
2015-05-09
Twórcy
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan, lem2403@mail.ru
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
Bibliografia
  • 1. Brenner B.M. editor (2003). Brenner and Rector’s The Kidney, 7th ed. Saunders. 2003.
  • 2. Chmielewski M. et al.: The role of epigenetics in kidney diseases. Contributions. Sec. Biol. Med. Sci., 1, 2011.
  • 3. Dolgushin I., Andreeva Y.: Method for detecting neutrophils extracellular traps: Patent of Russia Federation. № 2384844, 2008
  • 4. Fedorova M., Levin V.: The complex method of study geometry. surface area. Reserve capacity of the membrane and osmotic regulation of leukocyte. Clinical medicine, 8, 2000. (Article in Russian)
  • 5. Jaber B.L. et al.: Mechanisms of neutrophil apoptosis in uremia and relevance of the Fas (APO-1. CD95)/Fas ligand system. Journal of Leukocyte Biology, 69, 6, 2001.
  • 6. Kirchner T. et al.: The Impact of Various Reactive Oxygen Species on the Formation of Neutrophil Extracellular Traps. Mediators of Inflammation, Article ID 849136, 2012.[Crossref]
  • 7. Levine R.L. et al.: Determination of carbonyl content in oxidatively modified proteins. Method. Enzymol, 186, 1990.
  • 8. Markusheva L. et al.: Nuclear proteins of chromatin in assessing of the effectiveness of treatment of patients with psoriasis. Clinical laboratory diagnostics, 7, 2000. (Article in Russian)
  • 9. Mayadas T.N. et al.: Neutrophils: game changers in glomerulonephritis? Trends Mol. Med., 16(8), 2010.[Crossref][WoS]
  • 10. Median A.G. et al.: Differential carbonylation of proteins as a function of in vivo oxidative stress. J Proteome Res., 2; 10(9), 2011.[WoS]
  • 11. Musal K., Zwolinska D.: Immune system in chronic renal failure patients. Adv. Clin. Exp. Med, 12, 2, 2003.
  • 12. Davison A.M., Cameron J.S., Grunfeld J.-P., et.al. Editors (2005). Oxford Textbook of Clinical Nephrology. 3rd Ed. New York, Oxford University Press.
  • 13. Parker H. et al.: Myeloperoxidase associated with neutrophil extracellular traps is active and mediates bacterial killing in the presence of hydrogen peroxide. Journal of Leukocyte Biology, 91, N 3, 2012.[WoS][Crossref]
  • 14. Sharma A. et al.: Impaired function of neutrophils in uraemic patients. Natl Med J India, 3, 2000.
  • 15. Witko-Sarsat V. et al.: Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int., 49, 1996.
  • 16. Zawrotniak M., Rapala-Kozik M.: Neutrophil extracellular traps (NETs) - formation and implications. Acta Biochim. Pol., 60, (3), 2013.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_cipms-2015-0034
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