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2015 | 13 | 1 |
Tytuł artykułu

Analysis of oligonucleotides by liquid chromatography with alkylamide stationary phase

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The main aim of the present investigation was to determine retention behavior and interactions of oligonucleotides with alkylamide stationary phase. Five oligonucleotides, differing in the sequence, were tested. Changes in the composition of the mobile phase, i.e. pH (5.0−7.0) and buffer concentration (30−75 mM) were investigated in detail. In addition, the hydrophobic and electrostatic parameters were measured for the different pH’s and salt concentrations. The theoretical model of interactions between individual elements of the separation system, (e.g. solute, stationary phase, and mobile phase) based on zeta potential measurements, hydrophobic and electrostatic parameters calculation, and molecular modeling, has been considered.
EN
Wydawca

Czasopismo
Rocznik
Tom
13
Numer
1
Opis fizyczny
Daty
otrzymano
2015-01-16
zaakceptowano
2015-09-14
online
2015-11-25
Twórcy
  • Chair of the Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St. PL 87-100 Torun, Poland
autor
  • Chair of the Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St. PL 87-100 Torun, Poland
  • Chair of the Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St. PL 87-100 Torun, Poland
Bibliografia
  • [1] Azarani, A., Hecker, K.H.; RNA analysis by ion pari reversed phase high performance liquid chromatography; Nucleic Acids Research, (2001); 29: 7-10.
  • [2] Fountain, K.J., Gilar, M., Gebler, J.C.; Analysis of native and chemically modified oligonucleotides by tandem ion-pair reversed-phase high-performance liquid chromatography/electrospray mass spectrometry; Rapid Communication in Mass Spectrometry, (2003); 17: 646-653.
  • [3] Gilar, M., Bouvier, E.S.; Purification of crude DNA oligonucleotides by solid-phase extraction and reversed-phase high-performance liquid chromatography; Journal of Chromatography A, (2000); 890: 167-177.
  • [4] Beaucage, S.L.; Lyer, R.P.; Advances in the synthesis of oligonucleotides by the phosphoramidite approach; Tetrahedron, (1992); 48 2223-2311.[Crossref]
  • [5] Berg, J.M., Tymoczko, J.L.; Stryer, L; Biochemistry, 5th Edn., Freeman and Co., New York, 2000.
  • [6] Crooke, S.T.; Progress toward oligonucleotide therapeutics: pharmacodynamic properties; Faseb Journal, (1993); 7: 533-539.
  • [7] Crooke, S.T.; Molecular mechanisms of antisense drugs: RNase H, Antisense and Nucleic Acid Drug Development, (1996); 8: 133-134.
  • [8] McGinnis, A.C.; Chen, B; Bartlett, M.G.; Chromatographic methods for the determination of therapeutic oligonucleotides; Journal of Chromatography B, (2012); 883: 76-99.
  • [9] Gilar, M.; Fountain, K.J.; Budman, Y.; Holyoke, J.L.; Davoudi, H.; Gebler, J.C.; Characterization of therapeutic oligonucleotides using liquid chromatography with on-line mass spectrometry detection; Oligonucleotides; (2003); 13: 229-243.
  • [10] Gilar, M.; Fountain, K.J.; Budman, Y.; Neue, U.D.; Yardley, K.R.; Rainville, P.R.; Russel, R.J.; Gebler, J.C.; Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides: retention prediction; Journal of Chromatography A, (2002); 958: 167-182.
  • [11] Buszewski, B.; Schid, J.; Albert, K.; Bayer, E.; Chemically Bonded Phases for the Reversed-Phase High-Performance Liquid Chromatographic Separation of Basic Substances; Journal of Chromatography A, (1991); 552: 415-427.
  • [12] Michel, M.; Bączek, T.; Studzinska, S.; Bodzioch, K.; Jonsson, T.; Kaliszan, R.; Buszewski, B; Comparative evaluation of high-performance liquid chromatography stationary phases used for the separation of peptides in terms of quantitative structure–retention relationships; Journal of Chromatography A, (2007); 1175: 49-54.[WoS]
  • [13] Buszewski, B.; Kowalska, S.; Kowalkowski, T.; Rozpędowska, K.; Michel, M.; Jonsson, T.; HPLC columns partition by chemometric methods based on peptides retention; Journal of Chromatography B, (2007); 845: 253-260.[WoS]
  • [14] Buszewski, B.; Bocian, Sz.; Dziubakiewicz, E.; Zeta potential determination as a new way of stationary phases characterization for liquid chromatography; Journal of Separation Science, (2010); 33: 1529-1537.
  • [15] Buszewski, B.; Gadzała-Kopciuch, R.M.; Markuszewski, M.; Kaliszan, R.; New chemically bonded silica stationary phases: synthesis, physicochemical characterization and molecular mechanism of reversed-phase HPLC retention; Analytical Chemistry, (1997); 69: 3277-3284.
  • [16] Gadzała-Kopciuch, R.M.; Buszewski, B.; Comparative study of hydrophobicity of octadecyl and alkylamide bonded phases using the methylene selectivity; Journal of Separation Science, (2003); 26: 1273-1283.
  • [17] Buszewski, B.; Jaroniec, M.; Gilpin, R.K.; Influence of eluent composition on retention and selectivity of alkylamide phases under reversed-phase conditions; Journal of Chromatography A, (1994); 668, 293-299.
  • [18] Czajkowska, T.; Jaroniec, M.; Application of Alkylamide Phases to Separate Compounds of Different Polarity Under Reversed Phase Conditions; Journal of Liquid Chromatography & Related Technologies, (1996); 19, 2829-2841.
  • [19] Frisch, M.J.; Trucks, G.W.; Schlegel, H.B.; Scuseria, G.E.; Robb, M.A.; Cheeseman, J.R.; Gaussian, 2003.
  • [20] Dennington, I.R.; Keith, T.; Millam, J.; Eppinnett, K.; Hovell, W.L.; Gilliland, R.; GaussView, Version 3.09; Semichem, Inc.: Shawnee Mission, KS, 2003.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_chem-2015-0141
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