Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 13 | 1 |
Tytuł artykułu

Supercritical carbon dioxide hops extracts with antimicrobial properties

Treść / Zawartość
Warianty tytułu
Języki publikacji
Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 μg mL-1. Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of α-acids, β-acids, iso-α-acids and xanthohumol in the samples were determined by HPLC.

Opis fizyczny
  • New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
  • Institute for Medicinal Plant Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
  • University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobodjenja 18, 11000 Belgrade, Serbia
  • University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia
  • University of Niš, Faculty of Medicine, Dr Zoran Djindjić 81, 18000 Niš, Serbia
  • New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
  • New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
  • [1] Zanoli P., Zavatti M., Pharmacognostic and pharmacological profile of Humulus lupulus L., J. Ethnopharmacol., 2008, 116, 383-396.[WoS]
  • [2] Obara K., Mizutani M., Hitomi Y., Yajima H., Kondo K., Isohumulones, the bitter component of beer, improve hyperglycemia and decrease body fat in Japanase subjects with prediabetes, Clin. Nutr., 2009, 28, 278-284.[Crossref]
  • [3] Negri G., Di Santi D., Tabach R., Bitter acids from hydroethanolic extracts of Humulus lupulus L., Cannabaceae, used as anxiolytic, Rev. Bras. Farmacogn., 2010, 20, 850-859.[WoS][Crossref]
  • [4] Yamaguchi N., Satoh-Yamaguchi K., Ono M., In vitro evaluation of antibacterial, anticollagenase, and antioxidant activities of hop components (Humulus lupulus) addressing acne vulgaris, Phytomedicine, 2009, 16, 369-376.[WoS][Crossref]
  • [5] Srinivasan, V., Goldberg, D., Haas, G.J., Contributions to the antimicrobial spectrum of hop constituents, Econ. Bot., 2004. 58, S230-S238.
  • [6] Brown A.J., Clubb D., Antiseptic properties of hops, J. I. Brewing, 1913, 16, 261-295.[Crossref]
  • [7] Larson A.E., Yu R.Y.R., Lee O.A., Price S, Haas G.J, Johnson E.A., Antimicrobial activity of hop extracts against Listeria monocytogenes in media and in food, Inter. J. Food Microb.,1996, 33, 195-207.
  • [8] Shen C., Sofos J.N., Antilisterial activity of hops beta acids in broth with or without other antimicrobials, J. Food Sci., 2008, 73, M438-M442.[WoS]
  • [9] Arimoto-Kobayashi S., Takata J., Nakandakari N., Fujioka R., Okamoto K., Konuma T., Inhibitory effects of heterocyclic Amine - induced DNA adduct formation in mouse liver and lung by beer, J. Agric. Food Chem., 2005, 53, 812-815.
  • [10] Gerhäuser C., Broad spectrum anti-infective potential of xanthohumol from hop (Humulus lupulus L.) in comparison with activities of other hop constituents and xanthohumol metabolites, Mol. Nutr. Food Res., 2005, 49, 827-831.
  • [11] Teuber M., Schmareck A.F., Membrane leakage in Bacillus subtilis 168 induced by the hop constituents lupulone, humulone, isohumulone and humulinic acid, Arch. Mikrobiol., 1973, 94, 159-171.
  • [12] Schmalreck A.F., Teuber M., Reininger W., Hartl A., Structural features determining antibiotic potencies of natural and synthetic hop bitter resins, their precursors and derivatives, Can. J. Microbiol., 1975, 21, 205-212.[Crossref]
  • [13] Van Cleemput M., Cattoor K., De Bosscher K., Haegemang., De Keukeleire D., Heyerick A., Hop (Humulus lupulus)-derived bitter acids as multipotent bioactive compounds, J. Nat. Prod., 2009, 72, 1220-1230.[WoS]
  • [14] Teuber M., Low antibiotic potency of isohumulone, Appl. Microbiol., 1970, 19, 871.
  • [15] Yoshimaru T., Komatsu M., Tashiro E., Imoto M., Osada H., Miyoshi Y., et al., Xanthohumol suppresses oestrogen-signalling in breast cancer through the inhibition of BIG3-PHB2 interactions, Sci. Rep., 2014, 4, 7355, DOI: 10.1038/srep07355.
  • [16] Zhao F., Watanabe Y., Nozawa H., Daikonnya A., Kondo K., Kitanaka S., Prenylflavonoids and phloroglucinol derivatives from hops (Humulus lupulus), J. Nat. Prod., 2005, 68, 43-49.[Crossref]
  • [17] Kostrzewa D., Dobrzyńska-Inger A., Rój E., Experimental data on xanthohumol solubility in supercritical carbon dioxide, Fluid Phase Equilib., 2013, 360, 445-450.
  • [18] Adams R.P., Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th ed., Allured Publishing Corporation, Carol Stream, IL, USA, 2007.
  • [19] Automated Mass Spectral Deconvolution and Identification System software (AMDIS ver. 2.1National Institute of Standards and Technology (NIST), Standard Reference Data Program, Gaithersburg, MD, USA, 2005.
  • [20] Clinical and Laboratory Standards Institute, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved standard-6th edition. M7-A6, Clinical and Laboratory Standards Institute, Wayne, PA, USA, 14, 2003.
  • [21] Taniguchi Y., Matsukura Y., Ozaki H., Nishimura K., Shindo K., Identification and quantification of the oxidation products derived from α-acids and β-acids during storage of hops ( Humulus lupulus L.), J Agric Food Chem., 2013, 61, 3121-3130.[WoS]
  • [22] James P., Tynan T., Murrough I. Mc., Byrne J., Preparation, purification and separation by high performance liquid chromatography of humulinic acids, dehydrohumulinic acids, and hulupones, J. Inst. Brew., 1990, 96, 137-141.
  • [23] Zhang X., Liang X., Xiao H., Xu Q., Direct characterization of bitter acids in a crude hop extract by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry, J. Am. Soc. Mass Spectrom., 2004, 15, 180-187.
  • [24] Ligor M., Stankevičius M., Wenda-Piesik A., Obelevičius K., Ragažinskienė O., Stanius Ž., et al., Comparative gas chromatographic–Mass spectrometric evaluation of hop (Humulus lupulus L.) essential oils and extracts obtained using different sample preparation methods, Food Anal. Method., 2014, 7, 1433-1442.[WoS][Crossref]
  • [25] Helmja K., Vaher M., Püssa T., Kamsol K., Orav A., Kaljurand M., Bioactive components of the hop strobilus: comparison of different extraction methods by capillary electrophoretic and chromatographic methods, J. Chromatogr. A, 2007, 1155, 222-229.[WoS]
  • [26] Mizobuchi S., Sato Y., A new flavanone with antifungal activity isolated from hops, Agric. Biol. Chem., 1985, 48, 2771-2775.
  • [27] Lu X., Liu J., Hou F., Liu Z., Cao X., Seo H., Gao B., Cholesterol induces pancreatic β cell apoptosis through oxidative stress pathway, Cell Stress Chaperon., 2011, 16, 539-548. [WoS][Crossref]
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.