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2015 | 13 | 1 |
Tytuł artykułu

Influence of extraction methods on the recovery of astragaloside IV from the roots ofAstragalus mongholicusin Soxhlet- and Twisselmann-type apparatus

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Języki publikacji
EN
Abstrakty
EN
Astragalus mongholicus, commonly used in Traditional Chinese Medicine (TCM), contains significant amounts of triterpene saponins – astragalosides, which possess a wide variety of pharmacological actions, including the treatment of cardiovascular and respiratory diseases. The present study was performed to develop a fast and effective extraction method of saponins from crude plant material using Twisselmann-type apparatus. Moreover, a detailed comparison between Soxhlet and Twisselmann apparatus was performed to explain the advantages and disadvantages of their applications. The content of astragaloside IV – the major saponin of A. mongholicus extracts and chosen as an extraction efficiency marker ‒ was determined by means of RP-HPLCELSD chromatography in an herein optimized method. Twisselmann extraction shortened its duration from 4 to 2 hours in comparison with the Soxhlet method ‒ a generally accepted method preparation for this extract. The effectiveness of both apparatus was alike (3.24 ± 0.44 mg and 3.48 ± 0.26 mg of astragaloside IV/ 4g of powdered root for 2 hours in Twisselmann and 4 hours in Soxhlet apparatus). Compared with a pharmacopoeial method, the application of Twisselmann apparatus enabled a decrease in analysis time of 50%, which is of high significance, especially for large, scaled-up industrial scale.
EN
Wydawca
Czasopismo
Rocznik
Tom
13
Numer
1
Opis fizyczny
Daty
otrzymano
2014-12-03
zaakceptowano
2015-04-01
online
2015-06-02
Twórcy
  • Chair and
    Department of Pharmacognosy with Medical Plant Unit, Medical
    University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
autor
  • Chair and
    Department of Pharmacognosy with Medical Plant Unit, Medical
    University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Chair and
    Department of Pharmacognosy with Medical Plant Unit, Medical
    University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Chair and Department of Food and Nutrition, Medical
    University of Lublin, 4a, Chodźki Str., 20-093 Lublin, Poland
  • Chair and
    Department of Pharmacognosy with Medical Plant Unit, Medical
    University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Department of Industrial
    and Medicinal Plants, University of Life Sciences, 15, Akademicka Str.,
    20-950 Lublin, Poland
  • Department of Industrial
    and Medicinal Plants, University of Life Sciences, 15, Akademicka Str.,
    20-950 Lublin, Poland
  • Chair and
    Department of Pharmacognosy with Medical Plant Unit, Medical
    University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
Bibliografia
  • [1] Anon. Astragalus membranaceus. Altern. Med. Rev., 2007, 8, 72-77.
  • [2] Pharmacopoeia of the People’s Republic of China, Vol. 1, People’s Medical Publishing House, Shenzen, 2001.
  • [3] Yesilada E., Bedir E., Calis I., Takahashi Y., Ohmoto Y., Effects of triterpene saponins from Astragalus species on in vitro cytokine release, J. Ethnopharmacol., 2005, 96, 71-77.[Crossref]
  • [4] Lin L.Z., He X.G., Lindenmaier M., Nolan G., Yang J., Cleary M., et al., Liquid Chromatography-Electrospray Ionization Mass Spectrometry Study of the Flavonoids of the Roots of Astragalus mongholicus and A. membranaceus, J. Chrom. A, 2000, 876, 1-2, 87-95.
  • [5] Ren S., Zhang H., Mu., Sun M., Liu P., Pharmacological effects of Astragaloside IV: a literature review, J. Tradit. Chin. Med., 2013, 15, 33, 3, 413-416.
  • [6] Hu J.Y., Han J., Chu Z.G., Song H.P., Zhang D.X., Zhang Q., et al., Astragaloside IV attenuates hypoxia-induced cardiomyocyte damage in rats by upregulating superoxide dismutase-1 levels, Clin. Exp. Pharmacol. Physiol., 2009, 36, 4, 351-357.[Crossref][WoS]
  • [7] Ji K.T., Tang J.F., Chai J.D. Effect of Astragaloside against the oxidative damage on endothelial cells. Zhong Guo Zhong Xi Yi Jie He Za Zhi 2011, 31, 6, 807-810.
  • [8] Li H.B., Gei Y.K., Zhang L., Astragaloside IV improved barrier dysfunction induced by acute high glucose in human umbiIical vein endothehal cells. Life Sci. 2006; 79, 12, 1186-1193.
  • [9] Liu X., Min W., Protective effects of Astragaloside against ultraviolet a-induced photoaging in human fibroblasts, Zhong Xi Yi Jie He Xue Bao, 2011, 9, 3, 328-332.
  • [10] Meng L.Q., Tang J.W., Wang Y., Zhao J.R., Shang M.Y.,Zhang M., et al., Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy, Br. J. Pharmacol., 2011, 162, 8, 1805-1818.[WoS]
  • [11] Liu H., Wei W., Sun W.Y., Li X., Protective effects of Astragaloside IV on porcine-serum-induced hepatic fibrosis in rats and in vitro effects on hepatic stellate cells, J. Ethnopharmacol., 2009, 122, 3, 502-508.[WoS]
  • [12] Wang S., Li J., Huang H., Gao W., Zhuang C., Li B., et al., Anti-hepatitis B virus activities of Astragaloside IV isolated from radix Astragali, Biol. Pharm. Bull., 2009, 32, 1, 132-135.[Crossref][WoS]
  • [13] European Pharmacopoeia 8.0 edition, 2013.[PubMed]
  • [14] Monschein M., Ardjomand-Woelkart K., Rieder J., Wolf I., Heydel B., Kunert O., et. al., Accelerated sample preparation and formation of astragaloside IV in Astragali radix, Pharm Biol. (In press), DOI: 10.3109/13880209.2013.839712.[WoS][Crossref]
  • [15] Jalsrai A., Grecksch G., Becker A., Evaluation of the effects of Astragalus mongholicus Bunge saponin extract on central nervous system functions, J. Ethnopharmacol., 2010, 131, 3, 544-549.[WoS]
  • [16] Kloeck G., Noke A., Christiansen T., Soxhlet or Twisselmann, Filtr. Separat., 2013, 33, 32-33.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_chem-2015-0108
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