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2015 | 13 | 1 |
Tytuł artykułu

Retention, separation selectivity and system efficiency of selected basic psychotropic drugs on different RPLC columns

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Retention parameters of psychotropic drug standards were determined on different columns, i.e., Octadecyl silica, Phenyl, Phenyl-Hexyl, Polar Reverse Phase, Pentafluorophenyl, and Cyanopropyl using aqueous eluent systems containing methanol or acetonitrile as organic modifiers, acetate buffer at pH 3.5 and addition of silanol blocker − diethylamine (DEA). The retention, separation selectivity, and sequence of elution were different when using eluents containing various organic modifiers. The significant differences were observed in retention parameters with a change of the used stationary phase. The various properties of stationary phases resulted in differences in analyte retention, peaks shape, systems efficiency and separation selectivity. The best shape of peaks were on Cyanopropyl (CN) column and the highest efficiency for most investigated psychotropic drugs were obtained on Phenyl-Hexyl and Polar RP columns.
EN
Wydawca
Czasopismo
Rocznik
Tom
13
Numer
1
Opis fizyczny
Daty
otrzymano
2014-12-12
zaakceptowano
2015-04-01
online
2015-06-03
Twórcy
  • Department of Inorganic Chemistry Medical University
    of Lublin,Chodźki 4a, 20-093 Lublin, Poland
  • Department of Inorganic Chemistry Medical University
    of Lublin,Chodźki 4a, 20-093 Lublin, Poland
autor
  • Department of Inorganic Chemistry Medical University
    of Lublin,Chodźki 4a, 20-093 Lublin, Poland
  • Department of Inorganic Chemistry Medical University
    of Lublin,Chodźki 4a, 20-093 Lublin, Poland
Bibliografia
  • ---
  • [1] Ruiz-Angel M.J., Pous-Torresa S., Carda-BrocS., García-Alvarez-CoquM.C., Performance of different C18 columns in reversed-phase liquid chromatography with hydro-organic and micellar-organic mobile phases, J. Chromatogr. A, 2014, 1344, 76–82.
  • [2] Bella D. S., Jonesa A. D., Solute attributes and molecular interactions contributing to “U-shape” retention on a fluorinated high-performanceliquid chromatography stationary phase,J. Chromatogr. A, 2005, 1073, 99–109.
  • [3] Croes K., Steffens A., Marchand D.H., Snyder L.R., Relevance of p-p and dipole–dipole interactions for retention on cyano and phenyl columns in reversed-phase liquid chromatography,J. Chromatogr. A, 2005, 1098, 123–130.
  • [4] Stevenson P.G., Mayfield K.J., Solivena A., Dennis G.R., Gritti F., Guiochon G., Shalliker R.A., p-Selective stationary phases: (I) Influence of the spacer chain length of phenyl type phases on the aromatic and methylene selectivity of aromatic compounds in reversed phase high performance liquid chromatography,J. Chromatogr. A, 2010, 1217, 5358–5364.
  • [5] Stevenson P.G., Gritti F., Guiochon G., Mayfielda K.J., Dennis G.R., Shalliker R.A., p-Selective stationary phases: (II) Adsorption behaviour of substituted aromatic compounds on n-alkyl-phenyl stationary phases, J. Chromatogr. A, 2010, 1217, 5365–5376.[WoS]
  • [6] Yang M., Fazio S., Munch D., P. Drumm, Impact of methanol and acetonitrile on separations based on p–p interactions with a reversed-phase phenyl column, J. Chromatogr. A, 2005, 1097, 124–129.
  • [7] Choong E., Rudaz S., Kottelat A., Guillarme D., Veuthey J.-L., Eap C.B., Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC–MS, J. Pharm. Biomed. Anal., 2009, 50, 1000–1008.[WoS]
  • [8] Gajula R., Maddela R., Ravi V.B., Inamadugun J.K., Pilli N.R., A rapid and sensitive liquid chromatography–tandem mass spectrometric assay for duloxetine in human plasma: Its pharmacokinetic application, J. Pharm. Anal. 2013, 3, 36–44.
  • [9] Marchi I., Schappler J., Veuthey J.-L., Rudaz S., Development and validation of a liquid chromatography–atmospheric pressure photoionization–mass spectrometry method for the quantification of alprazolam, flunitrazepam, and their main metabolites in haemolysed blood, J. Chromatogr. B, 2009, 877, 2275–2283
  • [10] Marchei E., Escuder D., Pallas C.R., Garcia-Algar O., Gómez A., Friguls B., et al., Simultaneous analysis of frequently used licit and illicit psychoactive drugs in breast milk by liquid chromatography tandem mass spectrometry, J. Pharm. Biomed. Anal. 2011, 55, 309–316.
  • [11] Chaves A.R., Leandro F.Z., Carris J.A., Queiroz M.E.C., Microextraction in packed sorbent for analysis of antidepressants in human plasma by liquid chromatography and spectrophotometric detection, J. Chromatogr. B, 2010, 878, 2123–2129.[WoS]
  • [12] Sachse J., Koller J., Hartter S. , Hiemke C., Automated analysis of quetiapine and other antipsychotic drugs in human blood by high performance-liquid chromatography with column-switching and spectrophotometric detection, J. Chromatogr. B, 2006, 830, 342–348.
  • [13] Sampedro M.C., Unceta N., Gomez-Caballero A., Callado L.F., Morentin B., Goicolea M.A., Meana J.J., Barrio R.J., Screening and quantification of antipsychotic drugs in human brain tissue by liquid chromatography–tandem mass spectrometry: Application to postmortem diagnostics of forensic interest, Forensic Sci. Internat. 2012, 219, 172–178.
  • [14] Mercolini L., Mandrioli R., Iannello C., Matrisciano F., Nicoletti F., Raggi M.A., Simultaneous analysis of diazepam and its metabolites in rat plasma and brain tissue by HPLC-UV and SPE, Talanta 2009, 80 279–285.[WoS]
  • [15] Greiner C., Hiemke C., Bader W., Haen E., Determination of citalopram and escitalopram together with their active main metabolites desmethyl(es-)citalopram in human serum by column-switching high performance liquid chromatography (HPLC) and spectrophotometric detection, J. Chromatogr. B, 2007, 848, 391–394.
  • [16] Weigmann H., Hartter S., Hiemke C., Automated determination of clomipramine and its major metabolites in human and rat serum by high-performance liquid chromatography with on-line column-switching, J. Chromatogr. B, 1998, 710, 227 –233.
  • [17] Koeber R., Kluenemann H.-H., Waimer R., Koestlbacher A., Wittmann M., Brandl R., Doerfelt A., Jahner T., Melchner D., Haen E., Implementation of a cost-effective HPLC/UV-approach for medical routine quantification of donepezil in human serum, J. Chromatogr. B, 2012, 881– 882, 1– 11. [WoS]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_chem-2015-0106
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