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2015 | 13 | 1 |
Tytuł artykułu

Optimization of Data Acquisition and Sample Preparation Methods for LC-MS Urine Metabolomic Analysis

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Nowadays, chromatographic methods coupled with mass spectrometry are the most commonly used tools in metabolomics studies. These methods are currently being developed and various techniques and strategies are proposed for the profiling analysis of biological samples. However, the most important thing used to maximize the number of entities in the recorded profiles is the optimization of sample preparation procedure and the data acquisition method. Therefore, ultra high performance liquid chromatography coupled with accurate quadrupoletime- of-flight (Q-TOF) mass spectrometry was used for the comparison of urine metabolomic profiles obtained by the use of various spectral data acquisition methods. The most often used method of registration of metabolomics data acquisition – TOF (MS) was compared with the fast polarity switching MS and auto MS/MS methods with the use of multivariate chemometric analysis (PCA). In all the cases both ionization mode (positive and negative) were studied and the number of the identified compounds was compared. Additionally, various urine sample preparation procedures were tested and it was found that the addition of organic solvents to the sample noticeably reduces the number of entities in the registered profiles. It was also noticed that the auto MS/MS method is the least efficient way to register metabolomic profiles.
EN
Słowa kluczowe
Wydawca

Czasopismo
Rocznik
Tom
13
Numer
1
Opis fizyczny
Daty
otrzymano
2014-10-01
zaakceptowano
2015-01-01
online
2015-02-06
Twórcy
  • Department of Medicinal
    Chemistry, Pharmaceutical Faculty, Medical University of Lublin,
    Jaczewskiego 4, 20-090 Lublin, Poland, robert.skibinski@umlub.pl
  • Department of Medicinal
    Chemistry, Pharmaceutical Faculty, Medical University of Lublin,
    Jaczewskiego 4, 20-090 Lublin, Poland
Bibliografia
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  • [7] Iram M., Guo C., Guan Y., Ishfaq A., Liu H., Adsorption andmagnetic removal of neutral red dye from aqueous solutionusing Fe3O4 hollow nanospheres, J. Hazard. Mater., 2010, 181,1039–1050
  • [8] Wu J.S., Liu C.H., Chu K.H., Suen S.Y., Removal of cationic dyemethyl violet 2B from water by cation exchange membranes,J. Membrane Sci., 2008, 309, 239–245[WoS]
  • [9] Vîrlan C., Ciocârlan R. G., Roman T., Gherca D., Cornei N., PuiA., Studies on adsorption capacity of cationic dyes on severalmagnetic nanoparticles, Acta Chemica Iasi, 2013, 21, 19–30
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_chem-2015-0096
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