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Tytuł artykułu

Halogenation of β-estradiol by a rationally designed mesoporous biocatalyst based on chloroperoxidase

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Języki publikacji
EN
Abstrakty
EN
Chloroperoxidase from Caldariomyces fumago was immobilized in Eupergit® C, a commercial mesoporous acrylic-based material. Due to low stability of the enzyme under neutral and basic pH, the usual covalent immobilization procedures cannot be applied to this enzyme. Several strategies were followed in order to achieve a stable interaction between the protein and the support. The support was efficiently functionalized with different reactive groups such as aromatic and aliphatic amines, glutaraldehyde, diazonium ions, and maleimide moieties; solvent-exposed amino acid residues in chloroperoxidase were identified or created through chemical modification, so that they were reactive under conditions where the enzyme is stable. Enzyme load and retained activity were monitored, obtaining biocatalysts with specific activity ranging from 200 to 25,000 U/g. The highest load and activity was obtained from the immobilization of a chemically-modified CPO preparation bearing a solvent-exposed free thiol group. This biocatalyst efficiently catalyzed the transformation of β-estradiol, an endocrine disruptor.
Wydawca

Czasopismo
Rocznik
Tom
1
Numer
1
Opis fizyczny
Daty
otrzymano
2015-02-23
zaakceptowano
2015-04-05
online
2015-04-29
Twórcy
  • Instituto de Biotecnología, UNAM. Av. Universidad
    2001, Chamilpa 62210 Cuernavaca, Mor. México
  • Posgrado en Ciencias Ambientales,
    Instituto de Ciencias, Benemérita Universidad Autónoma
    de Puebla. Edificio 103 G Ciudad Univesitaria 72570. Puebla, Pue.
    México
autor
  • Posgrado en Ciencias Ambientales,
    Instituto de Ciencias, Benemérita Universidad Autónoma
    de Puebla. Edificio 103 G Ciudad Univesitaria 72570. Puebla, Pue.
    México
  • Instituto de Biotecnología, UNAM. Av. Universidad
    2001, Chamilpa 62210 Cuernavaca, Mor. México
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_boca-2015-0001
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