Angiogenic Effect of Bioactive Borate Glass Microfibers and Beads in the Hairless Mouse

• Autorzy: Richard J. Watters , Roger F. Brown , Delbert E. Day
• Języki publikacji: EN

Abstrakty

EN

The purpose of this project was to investigate the angiogenic mechanism of bioactive borate glass for soft tissue repair in a ‘hairless’ SKH1 mouse model. Subcutaneous microvascular responses to bioactive glass microfibers (45S5, 13-93B3, and 13-93B3Cu) and bioactive glass beads (13-93, 13-93B3, and 13-93B3Cu) were assessed via: noninvasive imaging of skin microvasculature; histomorphometry of microvascular densities; and quantitative PCR measurements of mRNA expression of VEGF and FGF-2 cytokines. Live imaging via dorsal skin windows showed the formation at twoweeks of a halo-like structure infused with microvessels surrounding implanted boratebased 13-93B3 and 13-93B3Cu glass beads, a response not observed with silicate-based 13-93 glass beads. Quantitative histomorphometry of tissues implanted with plugs of 45S5, 13-93B3, and 13-93B3Cu glass microfibers revealed microvascular densities that were 1.6-, 2.3-, and 2.7-times higher, respectively, than the sham control valueswhereas 13-93, 13-93B3, and 13-93B3Cu glass beads caused the microvascular density to increase 1.3-, 1.6-, and 2.5-fold, respectively, relative to sham controls. Quantitative PCR measurements indicate a marginally significant increased expression of VEGF mRNA in tissues with 13-93B3Cu glass beads, an outcome that supported the hypothesis that copper-doped borate glass could promote VEGF expression followed by angiogenesis for enhanced wound healing.

Słowa kluczowe

EN

Angiogenesis; copper-doped bioactive borateglass; bioactive glass microfibers; bioactive glass beads; invivo; soft tissue

• Czasopismo: Biomedical glasses
• Rocznik: 2015
• Tom: 1
• Numer: 1

Daty

otrzymano

2015-10-15

zaakceptowano

2015-12-05

online

2015-12-24

Twórcy

autor

Richard J. Watters

• Department of Biological Sciences and
  2Graduate Center for Materials Research, Missouri University of
  Science and Technology, Rolla, MO 65401

autor

Roger F. Brown

• Department of Biological Sciences and
  2Graduate Center for Materials Research, Missouri University of
  Science and Technology, Rolla, MO 65401

autor

Delbert E. Day

• Graduate Center for Materials Research Missouri
  University of Science and Technology

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Identyfikatory

DOI

10.1515/bglass-2015-0017