Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 1 | 1 |
Tytuł artykułu

Angiogenic Effect of Bioactive Borate Glass Microfibers and Beads in the Hairless Mouse

Treść / Zawartość
Warianty tytułu
Języki publikacji
The purpose of this project was to investigate the angiogenic mechanism of bioactive borate glass for soft tissue repair in a ‘hairless’ SKH1 mouse model. Subcutaneous microvascular responses to bioactive glass microfibers (45S5, 13-93B3, and 13-93B3Cu) and bioactive glass beads (13-93, 13-93B3, and 13-93B3Cu) were assessed via: noninvasive imaging of skin microvasculature; histomorphometry of microvascular densities; and quantitative PCR measurements of mRNA expression of VEGF and FGF-2 cytokines. Live imaging via dorsal skin windows showed the formation at twoweeks of a halo-like structure infused with microvessels surrounding implanted boratebased 13-93B3 and 13-93B3Cu glass beads, a response not observed with silicate-based 13-93 glass beads. Quantitative histomorphometry of tissues implanted with plugs of 45S5, 13-93B3, and 13-93B3Cu glass microfibers revealed microvascular densities that were 1.6-, 2.3-, and 2.7-times higher, respectively, than the sham control valueswhereas 13-93, 13-93B3, and 13-93B3Cu glass beads caused the microvascular density to increase 1.3-, 1.6-, and 2.5-fold, respectively, relative to sham controls. Quantitative PCR measurements indicate a marginally significant increased expression of VEGF mRNA in tissues with 13-93B3Cu glass beads, an outcome that supported the hypothesis that copper-doped borate glass could promote VEGF expression followed by angiogenesis for enhanced wound healing.

Opis fizyczny
  • Department of Biological Sciences and
    2Graduate Center for Materials Research, Missouri University of
    Science and Technology, Rolla, MO 65401
  • Department of Biological Sciences and
    2Graduate Center for Materials Research, Missouri University of
    Science and Technology, Rolla, MO 65401
  • Graduate Center for Materials Research Missouri
    University of Science and Technology
  • [1] Brown R., Watters R., Day D., Angiogenic Response of BioactiveBorate Glass Beads & Microfibers in “Hairless” Mice, TheAmerican Ceramic Society-Glass & Optical Materials Divisionand Deutsche Glastechnische Gesellschaft Joint Annual Meeting,Miami, FL, 17–21 May 2015.
  • [2] Chandan S., Gordillo M., Sashwati R., Kirsner R., Lambert L.,Hunt T.K., et al., Human skin wounds: a major and snowballingthreat to public health and the economy, Wound Repair Regen2009, 17, 763–771.[WoS][Crossref]
  • [3] Wu S., Driver V., Wrobel J., Armstrong D., Foot ulcers in thediabetic patient, prevention and treatment, Vasc Health RiskManag 2007, 3, 65–76.
  • [4] Caputo G., Cavanagh P., Ulbrecht J., Gibbons G., Karchmer A.,Assessment and management of foot disease in patients withdiabetes, New Engl J Med 1994, 13, 854-860.[Crossref]
  • [5] Keshaw H., Forbes A., Day R., Release of angiogenic growthfactor from cells encapsulated in alginate beads with bioactiveglass, Biomaterials 2005, 26, 4171–4179.[Crossref]
  • [6] Barralet J., Gbureck U., Habibovic P., Vorndran E., Gerard C.,Doillon C., Angiogenesis in calcium phosphate scaffolds by inorganiccopper ion release, Tissue Eng 2009, 15, 1601–1608.[Crossref]
  • [7] Li J., Zhang Y., Kirsner R., Angiogenesis in wound repair: Angiogenicgrowth factors and the extracellular matrix, Microsc ResTech 2003, 60, 107–114.[Crossref]
  • [8] Cole R., Liu F., Herron B., Imaging of angiogenesis: past, presentand future, In: A. Mendez – Vilas, J. Diaz (Eds.), Microscopy: Science,Technology, Applications and Education, Vol 3, Badajoz,Spain, 2010.
  • [9] Folkman J., Shing Y., Angiogenesis, J Biol Chem 1992, 267,10931–10934.
  • [10] Shih S., Robinson G., Perruzzi C., Calvo A., Desai K., Green J.,et al., Molecular profiling of angiogenesis markers, Am J Pathol2002, 161, 35–40.
  • [11] ClaytonW., Elasy T., A review of the pathophysiology, classification,and treatment of foot ulcers in diabetic patients, Clin Diabetes2009, 27, 52–58.[Crossref]
  • [12] Mohammad G., Pandey H., Tripathi K., Diabetic wound healingand its angiogenesis with special reference to nanoparticles,Dig J Nanomater Bios 2008, 3, 203–208.
  • [13] Falanga V., Wound healing and its impairment in the diabeticfoot, Lancet 2005, 366, 1736–43.
  • [14] Demidova-Rice T., Durham J., Herman I., Wound healing angiogenesis:innovations and challenges in acute and chronicwound healing, Adv Wound Care 2012, 1, 17–22.
  • [15] Papanas N., Efstratios M., Becaplermin gel in the treatment ofdiabetic neuropathic foot ulcers, Clin Interv Aging, Jun 2008,3(2), 233–240.
  • [16] Giavazzi R., Sennino B., Coltrini D., Garofalo A., Dossi R., RoncaR., et al., Distinct role of fibroblast growth factor-2 and vascular endothelial growth factor on tumor growth and angiogenesis,Am J Pathol, Jun 2003, 162, 1913–1926.[Crossref]
  • [17] Witkowski J., Parish L., Rational approach to wound care, Int JDermatol 1992, 31, 27–28.[Crossref]
  • [18] Fang R., Galiano R., A review of becaplermin gel in the treatmentof diabetic neuropathic foot ulcers, Biologics, 2008, 2, 1–12.
  • [19] Hoppe A., Mouriñob V., Boccaccini A., Therapeutic inorganicions in bioactive glasses to enhance bone formation and beyond,Biomater Sci 2013, 1, 254–256.[WoS][Crossref]
  • [20] Chen Q., Zhu C., Thouas G., Progress and challenges in biomaterialsused for bone tissue engineering: bioactive glasses andelastomeric composites, Prog Biomater 2012, 1(1), 2.[Crossref]
  • [21] Haro Durand L.A., Gongora A., Porto-Lopez J.M., Boccaccini A.R.,Zago M.P., Baldi A., et al., In vitro endothelial cell response toionic dissolution products from a boron-doped bioactive glassin the SiO2–CaO–P2O5–Na2O system, J Mater Chem B 2014, 2,7620–7630.[WoS][Crossref]
  • [22] Haro Durand L.A., Vargas G.E., Romero N.M., Vera-Mesones R.,Porto-Lopez J.M., Boccaccini A.R., et al., Angiogenic effects ofionic dissolution products released from a boron-doped 45S5bioactive glass, J Mater Chem B 2015, 3, 1142–1148.[Crossref][WoS]
  • [23] Jung S., Borate Based Bioactive Glass Scaffolds for Hard andSoft Tissue Engineering, Ph.D Dissertation, Missouri Universityof Science and Technology, Rolla, MO, 2010.
  • [24] Taylor P., personal communication, Phelps County RegionalMedical Center, Rolla, MO. 2012.
  • [25] Lin Y., Brown R., Jung S., Day D., Angiogenic effects of borateglass microfibers in a rodent model, J Biomed Mat Res A 2014,102(12), 4491–4499.
  • [26] Eliza M., Ben I., Reuven B., Histopathological periodic acid–schiff stains of nail clippings as a second-line diagnostic toolin onychomycosis, Am J Dermatopath 2012, 34, 270–273.[Crossref][WoS]
  • [27] Chomczynski P., Sacchi N., Single-step method of RNA isolationby acid guanidiniumthiocyanate-phenol-chloroform extraction,Anal Biochem 1987, 162, 156–9.[Crossref]
  • [28] Chomczynski P., Sacchi N., The single-step method of RNA isolationby acid guanidinium thiocyanate-phenol-chloroform extraction:twenty-something years on, Nat Protoc 2006, 1, 581–585.[Crossref]
  • [29] Wong M., Medrano J., Real-time PCR for mRNA quantitation,BioTechniques 2005, 39, 75–85.[Crossref]
  • [30] Livak K., Schmittgen T., Analysis of relative gene expressiondata using real-time quantitative PCR and the 2-
  • [Delta]
  • [Delta]Ctmethod, Methods 2001, 25, 402–408.
  • [31] Liu D., Handbook of Nucleic Acid Purification, CLC press, BocaRaton, FL. 2009.
  • [32] Zhao S., Li L., Wang H., Zhang Y., Cheng X., Zhou N., et al.,Wound dressings composed of copper-doped borate bioactiveglass microfibers stimulate angiogenesis and heal fullthicknessskin defects in a rodent model, Biomaterials 2015, 53,379–391.[WoS][Crossref]
  • [33] Kucan J., Robson M., Heggers J., Ko F., Comparison of silver sulfadiazine,povidone-iodine and physiologic saline in the treatmentof chronic pressure ulcers, J Am Geriatr Soc 1981, 29, 232–235.[Crossref]
  • [34] Harris E., A requirement for copper in angiogenesis, Nutr Rev2004, 62, 60–64.[Crossref]
  • [35] Hu G., Copper stimulates proliferation of human endothelialcells under culture, J Cellular Biochem 1998, 69, 326–335.[Crossref]
  • [36] Sen C.K., Copper-induced vascular endothelial growth factor expressionand wound healing, Am J Physiol 2002, 282, H1821–H1827.
  • [37] Emanueli C., Madeddu P., Angiogenesis gene therapy to rescueischemic tissues: achievements and future directions, BritJ Pharmacol 2001, 133, 951–958.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.