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EN
The objective of current study was to Statistical Comparison for Precision and Intermediate Precision study for Analytical Method Validation of Spironolactone and Furosemide in tablet formulation and developed easy, exact and correct isocratic stability indicating reversed phase HPLC assay method and validated for determination of Spironolactone and Furosemide in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on an SGE make 150 4.6mm SS Wakosil II 5C18RS 5 μm column (Part Number: 206610 and Serial Number: A01-063) using mobile phase of Acetonitrile- Ammonium acetate buffer (50:50, v/v) at a flow rate of 1.1 ml/min and the detection was carried out at 254 nm using photo-diode array detector. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug concentration range of 40-160 µg/ml with a correlation coefficient 0.9977 and 0.9953 for Spironolactone and Furosemide respectively. The precision (RSD) amongst six-sample preparation was 0.87% and 1.1 % for Spironolactone and Furosemide respectively. For repeatability and intermediate precision (RSD) amongst six-sample preparation was 0.46 % and 0.20 % for Spironolactone and Furosemide respectively. As result shown that for furosemide, % RSD was 1.12% and in ANOVA study Significance F value found 0.625502408 and for spironolactone Precision study and Intermediate precision study % RSD was 0.68, in ANOVA study Significance F value found 0.905843808.
EN
Some new 2-azetidinone derivatives possessing benzimidazole nucleus were synthesized and characterized by IR, NMR and mass spectral analysis. All synthesized compounds were screened for antimicrobial activity using cup plate method. All the compounds showed moderate to good antimicrobial activity and anti fungal activity.
EN
Synthesis of new thiazolidinone derivatives possessing benzimidazole nucleus by condensation reaction of various substituted mercapto acids in presence of anhydrous ZnCl2. In our present study we have also synthesized Schiff bases of benzimidazole derivatives. All synthesized compounds were characterized by IR, 1H NMR and Mass spectroscopy.
EN
The literature review reveal that [1,4]-oxazine derivatives represent one of the most active classes of compounds possessing wide spectrum of biodynamic activities and use as potent therapeutic agents. In the present work, a series of Schiff base of 2-(2,3-dihydro-2-oxonaphtho[2,1-b][1,4]oxazin-1-yl)acetohydrazide, 5a-5j has been synthesized using 1-aminonaphthalen-2-ol. Various aromatic aldehyde were react with carbohydrazide 4 in the presence of acid to produce the 2-oxonaphtho[2,1-b][1,4]oxazin Schiff base derivatives with good yields. All synthesized compounds were characterized by IR, NMR and Mass spectrometry analysis.
EN
Some novel 6-fluoro chroman derivatives having 1,2,4-triazolo-[3,4-b]thiadiazole were synthesized and characterized by IR, NMR and mass spectral analysis. All synthesized compounds were screened for antimicrobial activity using broth dilution method. All the compounds showed good antimicrobial activity and compound 5e showed significant antibacterial activity.
EN
Some new pyrimido[1,2-a]benzimidazole derivatives were synthesized by reacting 2-amino benzimidazole and chalcones in n-butanol at reflux temperature. In our present study we have used various heterocyclic chalcones derived from furfural and substituted acetophenones. All synthesized compounds were characterized by IR, 1H NMR and Mass spectroscopy. All synthesized compounds were screened for their antimicrobial activity against gram positive and gram negative bacteria which showed moderate to good activity.
EN
Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using Phenomenex Gemini c18 (4.6 x 250 mm, 5 μ) reverse phase column for the determination of LAF in human plasma, Solid Phase Extraction (SPE) technique was used for the extraction of analyte, detection was carried out by Photo Diode Array detector at 216 nm. Chromatographic resolution of the LAF was achieved within 4.6 min by using mobile phase Methanol and 5 mM Di-Potassium Hydrogen Phosphate Buffer (pH 9.5) (80:20, v/v), flow rate was 1.0 mL/min. Calibration curve was linear with correlation coefficient of 0.9996 in the range of 50-1000 ng/mL, Limit of Detection (LOD) and Limit of Quantitation (LOQ) were 10 ng/mL and 30 ng/mL respectively, intra and inter-day deviations were lower than 3.92 % and 3.98 % respectively. The overall mean recovery of LAF was 94.57 %. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC method was successfully validated and may be applied to conduct bioavailability & bioequivalence studies of LAF.
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