Currently, methods such as conventional ultrasound B-mode scanning (US), computerized X-ray tomography (CT), magnetic resonance imaging (MRI), standard X-ray diagnostics, radioisotope imaging and thermography are used to visualize the internal structure of tissue in vivo and to diagnose the patient. Doppler tomography (DT) is an innovative method of reconstructing the image of the tissue section using ultrasonic waves and Doppler effect. In contrast to the currently applied solutions (US), this method uses a continuous wave, which, in theory, allows one to operate with higher energy and to detect smaller inclusions within the examined tissue. This study focuses on the analysis of DT simulation in circular geometry, where a two-transducer ultrasonic probe circulating around the tested object is used to measure the useful signal. In this paper, the influence on the tested object’s cross-section imaging quality of both the simulated Doppler signal’s registration parameters, and the calculation algorithm’s parameters, were analyzed.