Biochar can get from every biomass material and carbonization of the total mass of organisms. Determining the most suitable dose of the biochar to increase nutrient concentrations of pepper and also determine whether used with chemical fertilizers or not was the aim of the study. In this study, biochar was used either with or without chemical fertilizers. Biochar was applied as 0, 10, 20, 40 t۰ha-1 into two liter pots containing two kilogram of soil. N-P-K was applied as 100 mg۰kg-1 N, 100 mg kg-1 P and 125 mg۰kg-1 K respectively. Leaf N, P, K, Fe, Mn, Cu, and Zn concentrations, soil pH, EC and organic material and plant dry weight were determined in the study. As a result, while biochar combined with chemical fertilizers, the soil pH and organic material increased, soil EC decreased, plant N, P, K, Fe, Mn and dry weight increased. Soil P and K concentrations also increased with the applications. From the results of the study, biochar could be used with chemical fertilizers to increase yield and concentrations of nutrients of pepper.